werner@aecom.UUCP (Craig Werner) (12/24/86)
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! Catalytic Antibodies
! Tramontano A, Janda KD, Lerner RA
! Science 234:1566 (19 Dec 1986)
Monoclonal antibodies elicited to haptens that are analogs of the
transition state for hydrolysis of carboxylic esters behaved as enzyme
catalysts with the appropriate substrates. These substrates are
distinguished by the structural congruence of both hydrolysis products
with haptenic fragments. The haptens were potent inhibitors of the
esterolytic activity, in agreement with their classification as
transition state analogs. Mechanisms are proposed to account for the
different chemical behavior of these antibodies with two types of ester
substrates. The generation of an artificial enzyme through transition
state stabilization by antibodies was thus demonstrated. These studies
indicate a potentially general approach to catalyst design.
[A related article immediately follows the above, entitled:
Selective Chemical Catalysis by an Antibody
Pollack SJ, Jacobs JW, Schultz PG.]
--
Craig Werner (MD/PhD '91)
!philabs!aecom!werner
(1935-14E Eastchester Rd., Bronx NY 10461, 212-931-2517)
"..pursuing Dharma, Artha, and Kama (although not nearly enough of the last)."