werner@aecom.UUCP (04/01/87)
! Megabase-Scale Mapping of the HLA Gene Complex
! by Pulsed-Field Gel Electrophoresis
! Lawrance SK, Smith CL, Srivastava R, Cantor CR, Weissman SM.
! Science (13 Mar 1987) 235:1387
In the study of the genetic structure of mammalian chromosomes,
there exists a "resolution gap" between molecular cloning experiments and
meiotic linkage analyses. This gap has discouraged attempts to construct
full-scale genetic maps of mammalian chromosomes. The organization of
the human major histocompatibility complex was examined within this range
by pulsed field gel electrophoresis,. The data obtained indicate that the
complex spans over 3000 kilobases and enable the construction of a
megabase-scale molecular map. The results indicate that the techniques
empolyed in DNA extraction, enzymatic digestion, electrophoresis, and
hybridization are suitable for the efficient analysis of megabase regions
of mammalian chromosomes and effectively bridge the resolution gap
between molecular cloning and classical genetics.
--
Craig Werner (MD/PhD '91)
!philabs!aecom!werner
(1935-14E Eastchester Rd., Bronx NY 10461, 212-931-2517)
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