diaz@aecom.UUCP (12/03/87)
Lee and Costlow (Methods in Enzymology 152, 633) describe the molecular
titration assay for measuring the prevalence of specific transcripts.
The method involves driving labeled RNA probes into RNA-RNA hybrids as
assayed by nuclease protection. A constant amount of probe is titrated
with increasing quantities of message. After nuclease digestion,
hybrids are collected on a filter and counted. A plot of counts vs RNA
concentration allows one to determine accurately the transcript
prevalence. The authors claim the method to be the most sensitive RNA
quantitation procedure.
If anyone has had experience with the procedure, I would appreciate
hearing from you.
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dn/dx Dept Molecular Biology diaz@aecom.yu.edu
Dan Diaz Albert Grubelstein College of Osteopathy