senyo@cmu.unige.ch (SENYO OFORI, DEPT. DE PHARMACOLOGIE, RUE MICHEL SERVET, 1211 GENEVE 4, SWITZERLAND. TEL: 0041-22-22 92 79) (07/11/90)
Hi everybody, I wonder if anyone could give me some ideas: I have an enzyme-dissociated population of bovine retinal neurons. The neuronal population consists of photoreceptors, bipolar cells, horizontal cells, ganglion cells as well as amacrine cells. Of the amacrine cells approximately 5% are dopaminergic. I am trying to isolate this subpopulation of amacrine cells from the total enzyme-dissociated cell population. My question is: Does anyone know of a specific dopaminergic cell surface antigen, to which there are commercially available antibodies? My intention is to coat a petri dish will this antibody and then pour the dissociated cells onto the plate, thereby specifically adsorbing the dopaminergic cells. Alternatively, does anyone know of a commercially available affinity column with a dopamine receptor antagonist (for example) attached, and which could be used to separate the dopaminergic neurons? Or finally, does anyone have any other idea as to how I could achieve my stated goal? Thanks in advance for your ideas. Senyo Ofori Department of Pharmacology Centre Medical Universitaire 1 Rue Michel Servet 1206 Geneva SWITZERLAND