phl01@ccu1.aukuni.ac.nz (Prof. Peter H Lovell) (06/24/91)
One of the researchers in my department is interested in software and hardware for 3-D reconstruction, where a series of images taken at successive depths through a specimen are "reconstructed" into a 3-D model of the specimen, so that it may be rotated and viewed from various different angles to reveal structures that might not have been visible in the original sections. I'd like to find out what software/hardware other people are using for this sort of work, and whether they have any recommendations. We'd prefer to use a high-end Macintosh for the job, although I'm also interested in hearing about solutions that use unix machines running X-windows, and PC-based solutions. (Is a high-end Mac powerful enough for this sort of work?) We're mainly interested in using 3-D reconstruction to study plant organs (i.e. images with lots of cells in them), though it would be a bonus if we could use it on larger structures (the sort people look at through stereo microscopes) and smaller structures such as cell organelles viewed via an electron microscope. Thanks in advance, Tim Hammett, Department of Botany, Auckland University, New Zealand. (phl01@ccu1.aukuni.ac.nz) -- Tim Hammett, Department of Botany, Auckland University, New Zealand. e-mail: phl01@ccu1.aukuni.ac.nz Phone: (+64)-9-737-999 x8344 -- Tim Hammett, Department of Botany, Auckland University, New Zealand. e-mail: phl01@ccu1.aukuni.ac.nz Phone: (+64)-9-737-999 x8344
wayne@helix.nih.gov (06/25/91)
In article <1991Jun23.214817.13956@ccu1.aukuni.ac.nz> phl01@ccu1.aukuni.ac.nz (Prof. Peter H Lovell) writes: > One of the researchers in my department is interested in software and > hardware for 3-D reconstruction, where a series of images taken at > successive depths through a specimen are "reconstructed" into a 3-D > model of the specimen, so that it may be rotated and viewed from > various different angles to reveal structures that might not have been > visible in the original sections. The public domain NIH Image program for the Mac, V1.40 or later, has the ability to reconstruct slices from serial sections. It is available via anonymous ftp from alw.nih.gov, in the directory /pub/image. DIP Station, also for the Mac, has the ability to generate 3-D models from serial sections. It is available from the Hayden Image Processing Group(whc@po.cwru.edu) in Cleveland, Ohio.
jlong@tamu.edu (James Long) (06/28/91)
In article <1991Jun23.214817.13956@ccu1.aukuni.ac.nz> phl01@ccu1.aukuni.ac.nz (Prof. Peter H Lovell) writes: > One of the researchers in my department is interested in software and > hardware for 3-D reconstruction, where a series of images taken at > successive depths through a specimen are "reconstructed" into a 3-D > model of the specimen, so that it may be rotated and viewed from > various different angles to reveal structures that might not have been > visible in the original sections. > > I'd like to find out what software/hardware other people are > using for this sort of work, and whether they have any recommendations. > We'd prefer to use a high-end Macintosh for the job, although I'm also > interested in hearing about solutions that use unix machines running > X-windows, and PC-based solutions. (Is a high-end Mac powerful enough > for this sort of work?) > > We're mainly interested in using 3-D reconstruction to study plant organs > (i.e. images with lots of cells in them), though it would be a bonus if > we could use it on larger structures (the sort people look at through > stereo microscopes) and smaller structures such as cell organelles > viewed via an electron microscope. If the mac + NIH Image are not powerful enough to do your reconstructions, you might consider a Silicon Graphics (Unix Workstation) and a software package called Voxel View from Vital Images. It does both 3-D reconstruction and volume redering. We are using it at this time to reconstruct data sets from a Zeiss Laser Scanning Confocal Microscope. It is a very nice package, but does represent a more sustantial investment - it depends on your needs and requirements for speed. I have seen it used to reconstruct slices of a paramecium in ice with striking results. I have more info if you need it, James Long jlong@emcnext2.tamu.edu (may bounce) snail mail: Electron Microscopy Center Texas A&M University College Station, Texas 77843-2257 USA
lawrance@ncsa.uiuc.edu (David Lawrance) (06/28/91)
Our group has written a program, called viewit, which runs on Unix boxes, best on those with lots of memory and fast cycles, and does volumetric rendering, among other functions (about 300). It and complete documentation are available on ftp.ncsa.uiuc.edu in the directory /unsupported/viewit. There are also Sunview and SGI display programs in there. It also works in conjunction with NCSA Telnet, DataScope and Image 3.0.