GenBank-Updates@genbank.bio.net (08/16/90)
LOCUS ECOPHOAA 600 bp ds-DNA BCT 16-AUG-1990 DEFINITION E.coli alkaline phosphatase (phoA) gene, 5' end. ACCESSION M33536 KEYWORDS alkaline phosphatase. SOURCE E.coli (strain K-12) cell line BW7710 DNA. ORGANISM Escherichia coli Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively anaerobic rods; Enterobacteriaceae. REFERENCE 1 (bases 1 to 600) AUTHORS Agrawal,D.K. and Wanner,B.L. TITLE A phoA structural gene mutation that conditionally affects formation of the enzyme bacterial alkaline phosphatase JOURNAL J. Bacteriol. 172, 3180-3190 (1990) STANDARD full staff_review COMMENT Draft entry and computer-readable sequence for [1] kindly submitted by D.K.Agrawal, 03-APR-1990. The phoA503 mutation does not interfere with export of active enzyme but does interfere with assembly. FEATURES from to/span description pept 283 > 600 alkaline phosphatase precursor (phoA) (EC 3.1.3.1) sigp 283 345 alkaline phosphatase signal peptide matp 346 > 600 alkaline phosphatase variant 413 413 c in wild type; t in phoA503 mutation BASE COUNT 159 a 130 c 151 g 160 t ORIGIN Map position 8.7 minutes; 1 bp upstream of HindIII site. 1 aagctttgga gattatcgtc actgcaatgc ttcgcaatat ggcgcaaaat gaccaacagc 61 ggttgattga tcaggtagag ggggcgctgt acgaggtaaa gcccgatgcc agcattcctg 121 acgacgatac ggagctgctg cgcgattacg taaagaagtt attgaagcat cctcgtcagt 181 aaaaagttaa tcttttcaac agctgtcata aagttgtcac ggccgagact tatagtcgct 241 ttgtttttat tttttaatgt atttgtacat ggagaaaata aagtgaaaca aagcactatt 301 gcactggcac tcttaccgtt actgtttacc cctgtgacaa aagcccggac accagaaatg 361 cctgttctgg aaaaccgggc tgctcagggc gatattactg cacccggcgg tgctcgccgt 421 ttaacgggtg atcagactgc cgctctgcgt gattctctta gcgataaacc tgcaaaaaat 481 attattttgc tgattggcga tgggatgggg gactcggaaa ttactgccgc acgtaattat 541 gccgaaggtg cgggcggctt ttttaaaggt atagatgcct taccgcttac cgggcaatac //