GenBank-Updates@genbank.bio.net (08/16/90)
LOCUS ECOPHOAA 600 bp ds-DNA BCT 16-AUG-1990
DEFINITION E.coli alkaline phosphatase (phoA) gene, 5' end.
ACCESSION M33536
KEYWORDS alkaline phosphatase.
SOURCE E.coli (strain K-12) cell line BW7710 DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 600)
AUTHORS Agrawal,D.K. and Wanner,B.L.
TITLE A phoA structural gene mutation that conditionally affects
formation of the enzyme bacterial alkaline phosphatase
JOURNAL J. Bacteriol. 172, 3180-3190 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by D.K.Agrawal, 03-APR-1990.
The phoA503 mutation does not interfere with export of active
enzyme but does interfere with assembly.
FEATURES from to/span description
pept 283 > 600 alkaline phosphatase precursor (phoA) (EC
3.1.3.1)
sigp 283 345 alkaline phosphatase signal peptide
matp 346 > 600 alkaline phosphatase
variant 413 413 c in wild type; t in phoA503 mutation
BASE COUNT 159 a 130 c 151 g 160 t
ORIGIN Map position 8.7 minutes; 1 bp upstream of HindIII site.
1 aagctttgga gattatcgtc actgcaatgc ttcgcaatat ggcgcaaaat gaccaacagc
61 ggttgattga tcaggtagag ggggcgctgt acgaggtaaa gcccgatgcc agcattcctg
121 acgacgatac ggagctgctg cgcgattacg taaagaagtt attgaagcat cctcgtcagt
181 aaaaagttaa tcttttcaac agctgtcata aagttgtcac ggccgagact tatagtcgct
241 ttgtttttat tttttaatgt atttgtacat ggagaaaata aagtgaaaca aagcactatt
301 gcactggcac tcttaccgtt actgtttacc cctgtgacaa aagcccggac accagaaatg
361 cctgttctgg aaaaccgggc tgctcagggc gatattactg cacccggcgg tgctcgccgt
421 ttaacgggtg atcagactgc cgctctgcgt gattctctta gcgataaacc tgcaaaaaat
481 attattttgc tgattggcga tgggatgggg gactcggaaa ttactgccgc acgtaattat
541 gccgaaggtg cgggcggctt ttttaaaggt atagatgcct taccgcttac cgggcaatac
//