GenBank-Updates@genbank.bio.net (08/16/90)
LOCUS RP4TRAB 571 bp ds-DNA BCT 16-AUG-1990
DEFINITION Plasmid RP4 traJ and traK genes, 5' end.
ACCESSION M25423
KEYWORDS inerted repeat; transfer origin region.
SOURCE Plasmid RP4 (strain HB101, IncP-alpha) DNA.
ORGANISM Plasmid RP4
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 571)
AUTHORS Lanka,E. and Euerste,J.P.
TITLE Conjugative transfer of promiscuous IncP plasmids: Interaction of
plasmid-encoded products with the transfer origin
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 86, 1771-1775 (1989)
STANDARD full staff_review
COMMENT Draft entry and printed sequence for [1] kindly submitted by
E.Lanka, 17-JUL-1989.
FEATURES from to/span description
pept 26 < 1 (c) traJ protein
pept 394 > 571 traK protein
rpt 48 63 inverted repeat
rpt 118 157 inverted repeat
signal 219 192 promoter PL
rpt 281 318 inverted repeat
signal 246 272 inverted repeat
BASE COUNT 135 a 148 c 181 g 107 t
ORIGIN
1 ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc ggcggtagcc
61 ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg
121 aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt
181 tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa
241 aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa
301 aagcgctgct tccctgctgt tttgtggaat atctaccgac tggaaacagg caaatgcagg
361 aaattactga actgagggga caggcgagag acgatgccaa agagctacac cgacgagctg
421 gccgagtggg ttgaatcccg cgcggccaag aagcgccggc gtgatgaggc tgcggttgcg
481 ttcctggcgg tgagggcgga tgtcgaggcg gcgttagcgt ccggctatgc gctcgtcacc
541 atttgggagc acatgcggga aacggggaag g
//