GenBank-Updates@genbank.bio.net (08/17/90)
LOCUS ECOORID 1890 bp ds-DNA BCT 17-AUG-1990
DEFINITION E.coli plasmid pColBM-Cl139 D protein gene, complete cds, oriV
region, and ORF.
ACCESSION J03312
KEYWORDS D protein; ORF; oriV region.
SOURCE E.coli (strain K12 Cl139) plasmid pColBM-Cl139 DNA.
ORGANISM Plasmid Colicin BM-Cl139
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 1890)
AUTHORS Thumm,G., Oelschlaeger,T. and Braun,V.
TITLE Plasmid pColBM-Cl139 does not encode a colicin lysis protein but
contains sequences highly homologous to the D protein (resolvase)
and the oriV region of the miniF plasmid
JOURNAL Plasmid 20, 75-82 (1988)
STANDARD simple staff_review
COMMENT Draft entry and sequence for [1] kindly submitted by G.Thumm,
18-AUG-1988. Sequence [1] complemented.
FEATURES from to/span description
pept 1108 1890 D protein
pept 654 1106 ORF8
site 257 576 oriV region
BASE COUNT 434 a 476 c 534 g 446 t
ORIGIN 1 bp upstream of EcoRI site.
1 gaattcatct tttggccgtt tacgtctgtt ccgttatcct gatgatacga tgttctgcac
61 gttctgccgg gaagatgcag atgattcgct taaaagtatt atgacccatc tctgggagct
121 ggatgcagag atgacagatc ctgtcatagc tatgtttaat cacgtctgag tgccgtgagt
181 gatttctgtc ttttatgcaa cagtgccaag atattgtaat caaaaaaaag cattaatgca
241 ttttggacag taatctattt taattgatga catagaggca ttaatctttc tttttcttca
301 ggaagatccg aaaactcctg gtcacggatc ttcctctccc ccacacaacg ccacctcctg
361 taagcacaac atgtggtgcc ggattcagct gctgatgaca ctatatgttg tgtcatctcc
421 ctgacctgtg atgcgtcgcg caggggcgga aaacagcgat atgatgattt cctcggcgtg
481 gtacacttcc ggaaagttgt gatattccgg aaagtcggat ctgacggaaa cggctctccg
541 gtaatttaac ggcgtggtta tatggatgct tgttatcatg gtgatgatga taacggcatg
601 atgttatcag acggcgtgac ggtaagggca gtgatgatgg atgacgttat cgcatgaccg
661 tccctgcccg gaaaagaaaa aaggagtcac ccatgttttt tattgagaat gaaggtcagg
721 ctgtcgccgg aacggattac tggcagtctg tacaggcgca ggccggatat gtctacctca
781 gctggaatgc cggcgcagcc aggctgcttg tcccggatgc ggcaaaacat ttactcaggg
841 agatgcgggg ggctgagtac gtcatcatca gtaagggagc actgcatggc cgcgatgcgc
901 tggaactggt atttgaagac ggcagcgatg cgccgtttgt gatccacatg ctgagtgagc
961 agtgcgatcg cctgctcccc gaaaacaacc agggaggggg ttttgttgtc accgtctgga
1021 cgcgtggcgg taaccagctc cgttatccgg gaaagtaccg ggttgtggaa aacctgcccg
1081 acgtttcccc gtggagtgaa cactgatatg cagcacctgc cggcaccgat ccaccatgcc
1141 cgggatgctg ttcagcttcc tgttgccatc gattatccgg cagcgctggc actccgccag
1201 atgtcgatgg ttcatgatga actgcccaaa tacctgctgg cccctgaagt gagcgccctg
1261 ctccattacg tcccggatct gcgccgcaag atgctgctgg ccacactgtg gaacaccggt
1321 gcgcgcatta atgaagcact ggcgctgacg cggggggatt tttcgctcac gcctccgtat
1381 ccgtttgtgc agctggccac tctgaagcag cggacagaaa aagccgccag gacggcagga
1441 agaatgcccg ccggtcagca gactcaccgg ctggttccgc tctccgactc ctggtacgtc
1501 agccagctgc agacgatggt agccacactg aaaatcccca tggaacggcg taataaacga
1561 acaggcagga cagagaaagc gcggatctgg gaagtgacgg acagaacggt caggacctgg
1621 attggggagg cggttgccgc cgctgccgct gatggtgtga cgttctctgt cccggtcacg
1681 ccacatacgt tccgccattc ctatgcgatg cacatgctgt atgccggtat accgcttaag
1741 gttctgcaga gtctgatggg gcataagtcc atcagctcaa cagaggtcta cacgaaggtg
1801 tttgcactgg atgtggctgc acggcaccgg gtgcagtttt cgatgcctga gtccgatgcg
1861 gtcacaatgc tgaaaaacag acatgcataa
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