GenBank-Updates@genbank.bio.net (08/17/90)
LOCUS ECOSPOT 3171 bp ds-DNA BCT 17-AUG-1990
DEFINITION E.coli spoT gene encoding (p)ppGpp 3'-pyrophosphohydrolase,
omega (rpoZ) protein genes, complete cds.
ACCESSION M24503 J04976
KEYWORDS (p)ppGpp 3'-pyrophosphohydrolase; SpoT protein; spoT gene.
SOURCE E.coli (strain K-12) DNA, clone pGA1.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 3171)
AUTHORS Sarubbi,E., Rudd,K.E., Xiao,H., Ikehara,K., Kalman,M. and Cashel,M.
TITLE Characterization of the spoT gene of Escherichia coli
JOURNAL J. Biol. Chem. 264, 15074-15082 (1989)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [1] kindly provided
by M.Cashel, 09-MAY-1989.
FEATURES from to/span description
pept 67 342 omega protein
pept 361 2469 (p)ppGpp 3'-pyrophosphohydrolase (ttg start)
ORF 2476 3165 ORF3
ORF 2934 880 (c) ORF4
BASE COUNT 784 a 850 c 857 g 680 t
ORIGIN 82 minutes on K12 map.
1 ttggcagact gaacctgatt tcagtatcat gcccagtcat ttcttcacct gtggagcttt
61 ttaagtatgg cacgcgtaac tgttcaggac gctgtagaga aaattggtaa ccgttttgac
121 ctggtactgg tcgccgcgcg tcgcgctcgt cagatgcagg taggcggaaa ggatccgctg
181 gtaccggaag aaaacgataa aaccactgta atcgcgctgc gcgaaatcga agaaggtctg
241 atcaacaacc agatcctcga cgttcgcgaa cgccaggaac agcaagagca ggaagccgct
301 gaattacaag ccgttaccgc tattgctgaa ggtcgtcgtt aatcacaaag cgggtcgccc
361 ttgtatctgt ttgaaagcct gaatcaactg attcaaacct acctgccgga agaccaaatc
421 aagcgtctgc ggcaggcgta tctcgttgca cgtgatgctc acgaggggca aacacgttca
481 agcggtgaac cctatatcac gcacccggta gcggttgcct gcattctggc cgagatgaaa
541 ctcgactatg aaacgctgat ggcggcgctg ctgcatgacg tgattgaaga tactcccgcc
601 acctaccagg atatggaaca gctttttggt aaaagcgtcg ccgagctggt agagggggtg
661 tcgaaacttg ataaactcaa gttccgcgat aagaaagagg cgcaggccga aaactttcgc
721 aagatgatta tggcgatggt gcaggatatc cgcgtcatcc tcatcaaact tgccgaccgt
781 acccacaaca tgcgcacgct gggctcactt cgcccggaca aacgtcgccg catcgcccgt
841 gaaactctcg aaatttatag cccgctggcg caccgtttag gtatccacca cattaaaacc
901 gaactcgaag agctgggttt tgaggcgctg tatcccaacc gttatcgcgt aatcaaagaa
961 gtggtgaaag ccgcgcgcgg caaccgtaaa gagatgatcc agaagattct ttctgaaatc
1021 gaagggcgtt tgcaggaagc gggaataccg tgccgcgtca gtggtcgcga gaagcatctt
1081 tattcgattt actgcaaaat ggtgctcaaa gagcagcgtt ttcactcgat catggacatc
1141 tacgctttcc gcgtgatcgt caatgattct gacacctgtt atcgcgtgct gggccagatg
1201 cacagcctgt acaagccgcg tccgggccgc gtgaaagact atatcgccat tccaaaagcg
1261 aacggctatc agtctttgca cacctcgatg atcggcccgc acggtgtgcc ggttgaggtc
1321 cagatccgta ccgaagatat ggaccagatg gcggagatgg gtgttgccgc gcactgggct
1381 tataaagagc acggcgaaac cagtactacc gcacaaatcc gcgcccagcg ctggatgcaa
1441 agcctgctgg agctgcaaca gagcgccggt agttcgtttg aatttatcga gagcgttaaa
1501 tccgatctct tcccggatga gatttacgtt ttcacaccgg aagggcgcat tgtcgagctg
1561 cctgccggtg caacgcccgt cgacttcgct tatgcagtgc ataccgatat cggtcatgcc
1621 tgcgtgggcg cacgcgttga ccgccagcct tacccgctgt cgcagccgct taccagcggt
1681 caaaccgttg aaatcattac cgctccgggc gctcgcccga atgccgcttg gctgaacttt
1741 gtcgttagct cgaaagcgcg cgccaaaatt cgtcagttgc tgaaaaacct caagcgtgat
1801 gattctgtaa gcctgggccg tcgtctgctc aaccatgctt tgggtggtag ccgtaagctg
1861 aatgaaatcc cgcaggaaaa tattcagcgc gagctggatc gcatgaagct ggcaacgctt
1921 gacgatctgc tggcagaaat cggacttggt aacgcaatga gcgtggtggt cgcgaaaaat
1981 ctgcaacatg gggacgcctc cattccaccg gcaacccaaa gccacggaca tctgcccatt
2041 aaaggtgccg atggcgtgct gatcaccttt gcgaaatgct gccgccctat tcctggcgac
2101 ccgattatcg cccacgtcag ccccggtaaa ggtctggtga tccaccatga atcctgccgt
2161 aatatccgtg gctaccagaa agagccagag aagtttatgg ctgtggaatg ggataaagag
2221 acggcgcagg agttcatcac cgaaatcaag gtggagatgt tcaatcatca gggtgcgctg
2281 gcaaacctga cggcggcaat taacaccacg acttcgaata ttcaaagttt gaatacggaa
2341 gagaaagatg gtcgcgtcta cagcgccttt attcgtctga ccgctcgtga ccgtgtgcat
2401 ctggcgaata tcatgcgcaa aatccgcgtg atgccagacg tgattaaagt cacccgaaac
2461 cgaaattaat gttttatgaa cccaacacgt tatgcacgca tctgcgaaat gctcgccagg
2521 cggcagcctg atctgaccgt ctgcatggag caggtccaca aacctcataa cgtttctgcg
2581 attattcgta ccgcagatgc cgttggcgta catgaagttc acgccgtctg gcctggtagc
2641 cgcatgcgca ccatggcttc ggcagcggcg ggtagtaaca gctgggtaca ggtgaaaaca
2701 caccgcacca ttggcgatgc cgtcgctcat ctcaaaggcc agggcatgca gattctggca
2761 acccatcttt ctgataacgc tgtcgatttc cgcgaaattg attacactcg cccgacctgc
2821 attttgatgg gacaggagaa aacgggcatc acgcaggaag cattggccct ggcggatcag
2881 gacatcatca ttccgatgat cggcatggtg cagtcgctga atgtttccgt tgcctcagcc
2941 ctcattcttt acgaagccca gcgtcagcgg caaaatgcag gcatgtacct gcgtgaaaac
3001 agcatgttgc cggaagcaga gcaacaacgc ctgttgtttg aaggcggcta tccggtgctg
3061 gcgaaagtcg caaaacgcaa aggcctgcct tatccccacg tcaatcagca aggcgagatc
3121 gaagctgatg ccgactggtg ggctactatg caggctgcag ggtaagtgcc a
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