GenBank-Updates@genbank.bio.net (08/17/90)
LOCUS PIGBNPA 1485 bp ds-DNA MAM 17-AUG-1990
DEFINITION Pig brain natriuretic peptide (BNP) primary transcript, complete
cds.
ACCESSION M25547 M22478 M22477 J04708
KEYWORDS brain natriuretic peptide.
SOURCE Pig atrium RNA, and cDNA to mRNA, clones 14b, 15a.
ORGANISM Sus scrofa
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Artiodactyla; Suiformes; Suidae.
REFERENCE 1 (bases 1 to 1485)
AUTHORS Porter,J.G., Arfsten,A., Palisi,T., Scarborough,R.M.,
Lewicki,J.A. and Seilhamer,J.J.
TITLE Cloning of a cDNA encoding porcine brain natriuretic peptide
JOURNAL J. Biol. Chem. 264, 6689-6692 (1989)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence [1] kindly submitted by
J.J. Seilhamer, 09-FEB-1989.
FEATURES from to/span description
pept 100 222 brain natriuretic peptide precursor, exon 1
469 724 brain natriuretic peptide precursor, exon 2
1279 1295 brain natriuretic peptide precursor, exon 3
sigp 100 168 brain natriuretic peptide signal peptide
matp 169 222 brain natriuretic peptide, exon 1
469 724 brain natriuretic peptide, exon 2
1279 1292 brain natriuretic peptide, exon 3
pre-msg < 7 1485 BNP mRNA and introns
IVS 223 468 BNP, intron A
IVS 725 1278 BNP, intron B
BASE COUNT 305 a 449 c 405 g 326 t
ORIGIN
1 gaattccagg ctgctaggaa gtgaaaagtg aacctggacc cagctcagcg gcagcagcag
61 cggcagcagg cagcagcctc tatcctctcc tccagccaca tgggcccccg gatggcgctt
121 ccccgcgtgc tcctgctcct gttcttgcac ctgttgctgc taggatgccg ttcccatcca
181 ctgggtggcg ctggcctggc ctcagaactg ccagggatac aggtgagccc tgatgaactg
241 cttagacttg gttggctggg agggcgcgga cagcagcaac taacgggtcc ccacctactg
301 ttccaagagg gctctaacct cctttgggaa ctagtgataa ggggttagaa ggcagccagg
361 ctgggggtga ggaccccgct cccaaggcag ttggttcgct tcagcaccat caagagtgat
421 gggtccaggt gcgagttcct gaggctcggg ctcccccacc catcccagga gctgctggac
481 cgcctgcgag acagggtctc cgagctgcag gcggagcgga cggacctgga gcccctccgg
541 caggaccgtg gcctcacaga agcctgggag gcgagggaag cagcccccac gggggttctt
601 gggccccgca gtagcatctt ccaagtcctc cggggaatac gcagccccaa gacgatgcgt
661 gactctggct gctttgggcg gaggctggac cggatcggct ccctcagcgg cctgggctgc
721 aatggtgagc acccaccccc attcccactg cacgccccgg ttagcatcac ttctgggttt
781 gatgtctctg gggaccaaac tccgagaaaa ggacacctgg atatcactct ttcttgttgc
841 cagtcctcaa ggccaaggag cgccttcctg gaaaaattaa atttggacag cattcactag
901 catgactatg agtccccacc caccttctcg ccaccccctg cctctctcac ccaaggcggc
961 agaattactt taggatgtaa attctgtcat tgcctggctg ccgctcctgg gagcaaaaag
1021 agaactaaac ctcttccccc tggtttcccc tcaactgtct gtggctgcaa aggcagaggg
1081 caggatcacc agggtgatga caagtcccag cttacaagga ggaaactcag gtccagagag
1141 atggattatc ccaaagcccc aaacatccag ttctgctgaa gaaggcgggt ggcaggggtg
1201 gcacgtggtg gggggaagcc caggtcctgc ctgcctctca ccctaatgtc atcctcaccc
1261 tctctctccc ccccacagtg ctcaggaggt actgagaagt cctggctgac aacctctgtg
1321 tccgcttctc caacgcccct cccctgctcc ccttcaaagc aactcctgtt tttatttatg
1381 tatttattta tttatttatt tggtggttgt atataagacg gttcttattt gtgagcacat
1441 tttttccatg gtgaaataaa gtcaacatta gagctctgtc ttttg
//