GenBank-Updates@genbank.bio.net (08/17/90)
LOCUS STYCYSJIHA 2194 bp ds-DNA BCT 17-AUG-1990
DEFINITION S.typhimurium NADPH-sulfite reductase flavoprotein component
(complete cds), and NADPH-sulfite reductase hemoprotein component
(5' end), genes.
ACCESSION M23007 M27145 J05025
KEYWORDS flavoprotein; hemoprotein; sulfite reductase.
SOURCE S.typhimurium (strain LT7, hisG70) DNA, clone pGBK5.
ORGANISM Salmonella typhimurium
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 370 to 2194)
AUTHORS Ostrowski,J., Barber,M.J., Rueger,D.C., Miller,B.E.,
Siegel,L.M. and Kredich,N.M.
TITLE Characterization of the flavoprotein moieties of NADPH-sulfite
reductase from Salmonella typhimurium and Escherichia coli:
Physicochemical and catalytic properties, amino acid sequence
deduced from DNA sequence of cysJ and comparison with
NADPH-cytochrome P-450 reductase
JOURNAL J. Biol. Chem. 264, 15796-15808 (1989)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 429)
AUTHORS Ostrowski,J. and Kredich,N.M.
TITLE Molecular characterization of the cysJIH promoters of Salmonella
typhimurium and Escherichia coli: Regulation by cysB protein and
N-acetyl-L-serine
JOURNAL J. Bacteriol. 171, 130-140 (1989)
STANDARD simple staff_review
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by N.M.Kredich, 16-SEP-1989.
FEATURES from to/span description
pept 390 2189 NADPH-sulfite reducatase flavoprotein
component (EC 1.8.1.2)
pept 2189 > 2194 NADPH-sulfite reductase hemoprotein component
(EC 1.8.1.2)
mRNA 219 > 2194 cysJIH mRNA (alt.)
mRNA 227 > 2194 cysJIH mRNA (alt.)
mRNA 228 > 2194 cysJIH mRNA (alt.)
BASE COUNT 490 a 583 c 601 g 520 t
ORIGIN 5 bp upstream of KpnI site.
1 ggtaccggaa agcgcatttt ttatcattaa ctaaaaccat tctccgcctt gtgacgggca
61 attaatttaa gaatttccag aaaatttaat aattatcaat caattataaa atcgattttg
121 gcgttaataa cgataactaa aacaggttag ttcatttggt tatttgttat ttccaaccct
181 tctttaattg ttattcctct caccgttaac cttatcctca gtttgggatt tatcgcttat
241 acatttcatc tttcaagccg catctgtgtt gactgcgttt actcacccca gtcacgtatt
301 tatgtacgct cctggggcgc gatcagctta cggcgaactt gaatgatttt gtgtatagat
361 ccgctttgct tactggaaca taacgacgca tgacgacacc ggctccactg actgggttgc
421 ttccgcttaa tccggagcaa ctggcgcgcc ttcaggccgc gacaaccgac cttactcccg
481 aacagcttgc ctgggtctcc ggctattttt ggggcgtact gaacccgcgc tcaggcgctg
541 ttgccgtcac gcctgttcct gagcggaaga tgccgcgggt tacgcttatc tccgcttcgc
601 aaaccggcaa tgcgcgtcgt gtggcggaag cgctgcgtga tgatctgctg gcggccaacc
661 ttaatgtgac actggtgaac gccggcgact acaaattcaa acagattgcc agtgaaaaac
721 tgttggtgat agtgacgtcc acccagggag aaggcgagcc gccggaagag gccgtcgcgc
781 tgcataaatt cctgttctcc aaaaaagcgc cgaaactgga aaacaccgct tttgccgtgt
841 ttagcctcgg cgacacctct tatgaatttt tctgccagtc cggaaaagat ttcgacagca
901 aactggcgga actgggcggt gaacgtctgc tcgaccgtgt cgatgctgac gttgaatatc
961 aggcagctgc cagcgaatgg cgcgcccgcg tggttgatgt tctgaagtct cgcgcacctg
1021 tcgcggctcc ttcgcaatcc gtcgctactg gcgcggtaaa cgacatccac accagcccct
1081 atactaaaga cgcgccgctg atcgcgaccc tctccgtgaa tcagaaaatc accggtcgca
1141 actcggaaaa agatgtgcgt catattgaga ttgatttagg cgactccggc ctgcgctatc
1201 agccgggcga tgcgctgggc gtctggtatc agaacgatcc ggctctggtg aaggagctgg
1261 ttgagttgct gtggctgaag ggcgatgagc cggtgacggt tgacggtaaa accctgccgc
1321 tcgccgaagc gctggagtgg cacttcgagc tgacggtgaa taccgccaac attgttgaga
1381 actacgcgac cctgacgcgc agtgagtcgc tgttgccatt agtgggcgac aaagcgcagc
1441 tccagcatta cgccgcgacc acgcccattg ttgatatggt gcgtttctct ccggcacagc
1501 tggatgctca ggcgctaatc gacctgctgc gtccgctaac gccgcgtctg tactccatcg
1561 cctccgcaca ggctgaagtg gagagcgaag tgcatatcac cgttggcgtg gttcgttatg
1621 acattgaagg ccgcgcccgg gccggtggcg cttccagctt cctcgccgac cgcgtggaag
1681 aagagggcga agttcgcgtc tttattgagc ataacgataa cttccgcctg ccagctaacc
1741 cggaaacgcc ggtgatcatg attggccccg gtaccggcat cgcgccgttc cgctccttta
1801 tgcaacagcg cgcggcggaa ggggtggaag gtaagaactg gttgttcttc ggcaacccgc
1861 actttaccga agatttcctc tatcaggtgg agtggcagcg ctacgtgaaa gagggggtgt
1921 taagccgtat cgatctcgcc tggtcacgcg atcaaaaaga aaaaatttac gtacaagaca
1981 agctgcgtga acagggcgcc gagctgtggc gctggatcaa tgatggcgcg catatttatg
2041 tctgcggcga cgccaggcgc atggccgcag atgtcgaaaa agcattgctg gaagtgatcg
2101 ctgaattcgg cggtatggac ctcgaatcgg cagatgaata tttaagtgag ctgcgcgtag
2161 agcgccgtta tcagcgagat gtctactaat gagc
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