GenBank-Updates@genbank.bio.net (08/17/90)
LOCUS TCKBM86A 2225 bp ss-mRNA INV 17-AUG-1990
DEFINITION B.microplus cell surface glycoprotein BM86 mRNA, complete cds.
ACCESSION M29321
KEYWORDS antigen; cell surface glycoprotein.
SOURCE B.microplus (strain Yeerongpilly ATCC 67543) intestine epithelial
cell, cDNA to mRNA, clone BTA1751.
ORGANISM Boophilus microplus
Eukaryota; Animalia; Metazoa; Arthropoda; Chelicerata; Arachnida;
Acari; Parasitiformes; Ixodida; Ixidoidea; Ixodidae.
REFERENCE 1 (bases 1 to 2225)
AUTHORS Rand,K.N., Moore,T., Sriskaantha,A., Spring,K., Tellam,R.,
Willadsen,P. and Cobon,G.S.
TITLE Cloning and expression of a protective antigen from the cattle tick
Boophilus microplus
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 86, 9657-9661 (1989)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by G.S.Cobon, 20-OCT-1989.
FEATURES from to/span description
pept 33 1985 cell surface glycoprotein BM86 precursor
sigp 33 89 cell surface glycoprotein BM86 signal peptide
matp 90 1912 cell surface glycoprotein BM86
mRNA < 1 2225 BM86 mRNA
signal 2203 2209 poly-A signal
rpt 24 65 EGF-like repeat
rpt 71 103 EGF-like repeat
rpt 113 147 EGF-like repeat
rpt 152 183 EGF-like repeat
rpt 209 246 EGF-like repeat
rpt 225 291 EGF-like repeat
rpt 295 334 EGF-like repeat
rpt 492 529 EGF-like repeat
rpt 535 567 EGF-like repeat
rpt 576 600 EGF-like repeat
BASE COUNT 666 a 492 c 574 g 493 t
ORIGIN
1 ccgcgacagc tgcggtggtt cgacgcagtg agatgcgtgg catcgctttg ttcgtcgccg
61 ctgtttcact gattgtagag ggcacagcag aatcatccat ttgctctgac ttcgggaacg
121 agttctgtcg caacgctgaa tgtgaagtgg tgcctggtgc agaggatgat ttcgtgtgca
181 aatgtccgcg agataatatg tacttcaatg ctgctgaaaa gcaatgcgaa tataaagaca
241 cgtgcaagac aagggagtgc agctatggac gttgcgttga aagtaacccg agcaaggcta
301 gctgcgtctg cgaagcatcg gacgatctaa cgctacaatg caaaattaaa aatgactacg
361 caactgactg ccgaaatcga ggtggcactg ctaagttgcg cacggatggg tttattggcg
421 caacgtgtga ctgtggtgaa tggggtgcga tgaacatgac cacccggaac tgtgtcccta
481 ccacgtgtct tcgtcccgac ttgacctgca aagacctctg cgagaaaaac ctgcttcaaa
541 gggattctcg ttgttgccag gggtggaaca cagcaaactg ttcagccgct cctccagctg
601 actcctattg ctctcctggg agccccaaag gaccggacgg acagtgtata aatgcttgca
661 agacgaaaga agctgggttt gtctgcaagc atggatgcag gtcgaccggc aaggcgtacg
721 agtgcacgtg cccgagtggc tctaccgtcg ccgaagatgg cattacctgc aaaagtattt
781 cgcacacagt cagctgcact gctgagcaaa aacagacctg ccgcccaacc gaagactgtc
841 gtgtgcacaa aggaactgtg ttgtgtgagt gcccgtggaa tcaacatcta gtgggggaca
901 cgtgcataag tgattgcgtc gacaagaaat gccacgaaga atttatggac tgtggcgtat
961 atatgaatcg acaaagctgc tattgtccat ggaaatcaag gaagccgggc ccaaatgtca
1021 acatcaatga atgcctactg aatgagtatt actacacggt gtcattcacc ccaaacatat
1081 cttttgattc tgaccattgc aaatggtatg aggatcgtgt tttggaagcg atacggacca
1141 gtatcggaaa agaagttttt aaggttgaga tacttaactg cacgcaggac attaaggcaa
1201 gactcatagc agagaaacca ctgtcaaaac acgtgctcag gaaactacaa gcatgcgagc
1261 atccaatcgg cgaatggtgc atgatgtatc cgaagttgct gatcaagaaa aactctgcaa
1321 cagaaatcga agaagagaac ctttgcgaca gtctgctcaa ggatcaggaa gctgcctaca
1381 aaggtcaaaa caaatgcgtc aaggtcgaca acctcttctg gttccagtgc gctgatggtt
1441 acacaacaac ttacgagatg acacgaggtc gcctacgccg ctccgtgtgt aaagctggag
1501 tttcttgcaa cgaaaacgag cagtcggagt gtgctgacaa agggcaaata tttgtttacg
1561 aaaacggcaa agcgaattgc caatgcccac cagacactaa acctggggag attggctgca
1621 ttgagcgtac cacatgcaac cctaaagaaa tacaagaatg ccaagacaag aagctggagt
1681 gcgtttacaa aaaccataaa gcagaatgcg agtgtcctga tgatcacgag tgttacaggg
1741 agcctgccaa agactcttgc agtgaagagg ataatggtaa atgtcaaagc agtgggcagc
1801 gttgtgtaat agaaaacgga aaggctgttt gcaaggaaaa gtctgaagca acaacagctg
1861 cgactacaac aacgaaagcg aaagacaagg atccagatcc tggaaagtca agtgctgcag
1921 cagtatcagc tactgggctc ttgttactgc tcgcagctac ttcagtcacc gcagcatcgt
1981 tgtaaggaag atgtccaact tgaatacgga acagcttgaa tatgtatata tacatcacgc
2041 ttacatcgaa cacctagctt ggtttttgga atttcaatat tgcgcattgg tactcacggc
2101 aacatgaatg tattacttta aaatgacagg gaagagggac gtgaaaggag tttccttgtc
2161 tgaacatatc aaagaaaatt ttcccctatc cgaccgatgt caaataaaga tagttgggtc
2221 taaac
//