GenBank-Updates@genbank.bio.net (08/17/90)
LOCUS RATPHOCAMA 1963 bp ss-mRNA ROD 17-AUG-1990
DEFINITION Rat cAMP phosphodiesterase (PDE3) mRNA, complete cds.
ACCESSION M25349 M28412
KEYWORDS phosphodiesterase.
SOURCE Rat (strain Sprague-Dawley, 15-day old) Sertoli cell, cDNA to mRNA,
clone ratPDE3.[1,2].
ORGANISM Rattus norvegicus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 556 to 1635)
AUTHORS Swinnen,J.V., Joseph,D.R. and Conti,M.
TITLE Molecular cloning of rat homologues of the Drosophila melanogaster
cAMP phosphodiesterase: Evidence for a family of genes
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 86, 5325-5329 (1989)
STANDARD full staff_entry
REFERENCE 2 (bases 1 to 1963)
AUTHORS Swinnen,J.V., Joseph,D.R. and Conti,M.
TITLE The mRNA encoding a high-affinity cAMP phosphodiesterase is
regulated by hormones and cAMP
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 86, 8197-8201 (1989)
STANDARD simple staff_entry
REFERENCE 3 (bases 556 to 1635)
AUTHORS Swinnen,J.V., Joseph,D.R. and Conti,M.
TITLE Molecular cloning of rat homologues of the Drosophila melanogaster
dunce cAMP phosphodiesterase: Evidence for a family of genes
JOURNAL Proc. Natl. Acad. Sci. U.S.A. (1989) In press
STANDARD full staff_review
COMMENT Draft entry and computer-readable copy of sequence [3] kindly
submitted by M.Conti, 27-SEP-1989.
Draft entry and computer-readable sequence for [1] kindly provided
by M. Conti, 06-JUN-1989.
An 86 bp deletion (positions 201-288) was found in clone PDE3.2.
Whether this is due to alternative splicing and therefore
physiologically relevant is not known at this time.
FEATURES from to/span description
pept 151 1905 cAMP phosphodiesterase
BASE COUNT 527 a 514 c 496 g 426 t
ORIGIN
1 ccgccgtccc cgtgccaaag ttggccttgc agcactttgc gcccctccct ggaggctgac
61 ccgcgacgtc aagctggagc atctcggcca gcctgagccc ggccccacta atccaacctc
121 agccccccgc gcgccgccgg gctctgcaat atgaaggagc agccctcatg tgcgggcacc
181 gggcatccga gcatggcggg gtacggcagg atggccccct ttgaactcgc tggtggtccg
241 gtgaagcgct taagaactga gtcccccttt ccctgtctct tcgcagagga ggcctaccag
301 aaactggcca gcgagaccct ggaggagctg gactggtgtc tggaccagct agagaccctg
361 cagaccaggc actccgtcag tgagatggcc tccaacaagt tcaagaggat gcttaatcgg
421 gagctcaccc acctctctga aatgagtcgg tctggcaacc aggtgtcgga gtacatatca
481 aacacgttct tagataagca acatgaagta gaaattccct ctccgactca gaaggaaaaa
541 gagaagaaga aaaggccgat gtcacagatc agtggggtca agaagttgat gcacagctcc
601 agcctgacca attcctgcat tccaagattt ggggttaaaa cagagcagga agatgtcctg
661 gccaaggaac tagaagacgt gaacaagtgg ggcctccacg ttttccgaat agcggagctg
721 tctggcaacc ggcctctgac tgttatcatg cacaccattt ttcaggaacg agatttgtta
781 aaaacgttta aaatcccagt ggacactttg attacgtatc ttatgactct agaagaccat
841 taccatgctg acgtggccta tcacaacaac atccatgctg cagatgtcgt ccagtcaact
901 catgtgctgc tctctacacc cgctttggag gctgttttca ctgacttgga gattctcgcg
961 gccatttttg ccagtgcaat acatgatgtg gatcatcctg gtgtgtcaaa tcaatttctg
1021 atcaatacaa actcggaact tgccttgatg tacaacgact cctccgtctt agagaatcat
1081 catttggctg tgggctttaa gttgctccag gaagaaaact gtgacatttt ccagaatctg
1141 accaaaaagc aaagacaatc tttaaggaaa atggccattg acattgtact agcgacagac
1201 atgtcaaagc acatgaatct gctggctgat ctgaaaacaa tggttgaaac gaagaaggtg
1261 acgagctctg gcgtcctcct ccttgataac tattctgaca ggatccaggt cctccagaat
1321 atggtgcact gtgcagacct gagcaacccc acaaagccac tccagctcta ccgccagtgg
1381 acggaccgga taatggagga gttcttccgt cagggggacc gggagcgtga gcgtggcatg
1441 gagataagtc ccatgtgtga caagcacaac gcctctgtgg aaaaatcaca ggtgggcttc
1501 atagactaca ttgttcatcc actctgggag acatgggcag acctcgtaca tcccgacgcc
1561 caggacattt tggacacttt ggaggacaat cgtgagtggt accagagcac aatcccccag
1621 agcccctccc ctgcacctga tgaccaagag gacggccgtc agggacagac tgaaaaattc
1681 cagttcgaac taaccttaga ggaagatggc gagtcagaca ctgaaaagga cagtggaagt
1741 caagtggagg aagacactag ctgcagtgac tctaagactc tgtgcaccca agactcagag
1801 tccactgaaa ttccccttga cgagcaggtt gaagaggagg ctgtagcaga agaggaaagc
1861 cagccccaaa ctggcgtcgc agacgattgt tgtcctgaca cgtaacagtg taaaggctgt
1921 cacacctttt ctttttcttt ttttttttta aagtaaaaaa aaa
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