GenBank-Updates@genbank.bio.net (08/17/90)
LOCUS ECOASNB 3080 bp ds-DNA BCT 17-AUG-1990
DEFINITION E.coli asparagine synthetase B (asnB) gene, complete cds.
ACCESSION J05554
KEYWORDS asparagine synthetase B.
SOURCE E.coli (K12) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 812 to 2912)
AUTHORS Scofield,M.A., Lewis,W.S. and Schuster,S.M.
TITLE Nucleotide sequence of Escherichia coli asnB and deduced amino acid
sequence of asparagine synthetase B
JOURNAL J. Biol. Chem. 265, 12895-12902 (1990)
STANDARD full staff_entry
REFERENCE 2 (bases 1 to 3080)
AUTHORS Scofield,M.A., Lewis,W.S. and Schuster,S.M.
JOURNAL Unpublished (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [2] kindly submitted
by M.A.Scofield, 06-JUL-1990.
FEATURES from to/span description
pept 1001 2665 asparagine synthetase B (asnB) (EC 6.3.5.4)
mRNA 956 2661 asnB mRNA
signal 943 948 -10 region
signal 920 925 -35 region
binding 1017 1037 ribosome binding site
BASE COUNT 786 a 766 c 775 g 753 t
ORIGIN 82 minutes.
1 agatctggcg aaccgctttg ccaccgcagg tgtcgatgta cctgacagcg tgttttatac
61 ctctgcgatg gcgactgccg atttcctgcg tcgccaggaa ggcaagaaag cgtatgtggt
121 gggcgaaggc gcactgattt atgaactgta caaagccggt ttcactatta ccgatgtgaa
181 ccctgatttc gtgattgttg gcgaaacgcg ttcctacaac tgggacatga tgcataaagc
241 agcctatttc gtcgctaacg gtgcacgttt tatcgccacc aatccggaca cccacgggcg
301 cggtttttat cccgcttgtg gcgcgttgtg tgcagggatt gagaaaatct ccgggcgcaa
361 accgttctat gttggtaagc ccagcccgtg gatcatccgc gcagcattaa acaaaatgca
421 ggcgcattcg gaagaaacgg tgattgtcgg cgataacctg cgtaccgata ttctggccgg
481 cttccaggca ggtctggaga cgattctggt gctttctggt gtttcgtcgc tcgacgatat
541 cgacagtatg cctttccgcc ccagctggat ttacccgtca gtcgctgaaa tcgacgttat
601 ctgaatagca accacgtctg cggacgtggt tggctctgat gacaaacgcc aaactgcctg
661 atgcgctacg cttatcaggc ctacgcagct cctgcaatat attgaatttt catgcttttg
721 taggccggat aaggcgttca cgccgcatcc ggcatgaaca aatcgcactt tgtcagcaat
781 ttggcccccg taaaatataa acattcaata aaacccgcta aaaattatcg attcatcaat
841 aaatatatcc tcacaccaga ccttttttca ccaatcgaca atcaccatta cgttttttat
901 tttttcaccg ccaaatcgct tgcgcacagt gctgctttgc ggcatttttt taaacaagca
961 aacacaacaa gcaacaaata ccaggttaac ggagaaggtt atgtgttcaa tttttggcgt
1021 attcgatatc aaaacagacg cagttgagct gcgtaagaaa gccctcgagc tgtcacgcct
1081 gatgcgtcat cgtggcccgg actggtccgg tatttatgcc agcgataacg ccattctcgc
1141 ccacgaacgt ctgtcaattg ttgacgttaa cgcgggggcg caacctctct acaaccaaca
1201 aaaaacccac gtactggcgg taaacggtga aatctacaac caccaggcat tgcgcgccga
1261 atatggcgat cgttaccagt tccagaccgg gtctgactgt gaagtgatcc tcgcgctgta
1321 tcaggaaaaa gggccggaat ttctcgacga cttgcagggc atgtttgcct ttgcactgta
1381 cgacagcgaa aaagatgcct acctgattgg tcgcgaccat ctggggatca tcccactgta
1441 tatggggtat gacgaacacg gtcagctgta tgtggcctca gaaatgaaag cgctggtgcc
1501 agtttgccgc acgattaaag agttcccggc ggggagctat ttgtggagcc aggacggcga
1561 aatccgttct tactatcatc gcgactggtt cgactacgat gcggtgaaag ataacgtgac
1621 cgacaaaaac gagctgcgtc aggcactgga agattcagtt aaaagccatc tgatgtctga
1681 tgtgccttac ggtgtgctgc tttctggtgg tctggattcc tcaattattt ccgctatcac
1741 caagaaatac gcagcccgtc gcgtggaaga tcaggaacgc tctgaagcct ggtggccgca
1801 gttacactcc tttgctgtag gtctgccggg ttcaccggat ctgaaagcag cccaggaagt
1861 ggcaaaccat ctcggcacgg tgcatcacga aattcacttc actgtacagg aaggtctgga
1921 tgccatccgc gacgtgattt accacatcga aacttatgat gtgaccacta ttcgcgcttc
1981 aacaccgatg tatttaatgt cgcgtaagat caaggcgatg ggcattaaaa tggtgctgtc
2041 cggtgaaggt tctgatgaag tgttcggcgg ttatctttac ttccacaaag caccgaatgc
2101 caaagaactg catgaagaga cggtgcgtaa actgctggcc ctgcatatgt atgactgcgc
2161 gcgtgccaac aaagcgatgt cagcctgggg cgtggaagca cgcgttccgt tcctcgacaa
2221 aaaattcctt gatgtggcga tgcgtattaa cccacaggat aaaatgtgcg gtaacggcaa
2281 aatggaaaaa cacatcctgc gtgaatgttt tgaagcgtat ctgcctgcaa gcgtggcctg
2341 gcggcagaaa gagcagttct ccgatggcgt cggttacagt tggatcgaca ccctgaaaga
2401 agtggctgcg cagcaggttt ctgatcagca actggaaact gcccgcttcc gcttcccgta
2461 caacacgcca acctctaaag aagcgtactt gtatcgggag atctttgaag aactattccc
2521 gcttccgagc gccgctgagt gcgtgccggg cggtccttcc gtcgcttgtt cttccgctaa
2581 agcgatcgaa tgggatgaag cgttcaagaa aatggacgat ccgtctggtc gcgcggttgg
2641 tgttcaccag tcggcgtata agtaagaaat gcgaaacagc cccggtgaaa tccggggctg
2701 tttcagttat tgaggtgctt gttgattctg atatgcagta aggaaatgtt ccgggtttgc
2761 cacatcactg tttgacaact catcactgta ggccagtatt tcatagatta atttcccacc
2821 actattgtta aattccgaat ttcatcctgt atgacaaaag aatggctgta gggtgggaaa
2881 aaacacaaag taaacaataa ttgacgaata tagcgccacg ctgttcgcaa cctaaccaaa
2941 cagtcacttt cgagcaattt tccttgaaaa agaggttgac gctgcaaggc tctatacgca
3001 taatgcgccc cgcaacgccg ataaggtatc gcgaaaaaaa agatggctac gtagctcagt
3061 tggttagagc acatcactca
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