GenBank-Updates@genbank.bio.net (08/18/90)
LOCUS YSCAMS1B 5189 bp ds-DNA PLN 18-AUG-1990
DEFINITION Yeast (S.cerevisiae) AMS1 gene encoding alpha-mannosidase, complete
cds.
ACCESSION M29146 M27809
KEYWORDS alpha-D-mannosidase; glycosidase.
SOURCE S.cerevisiae (strain X2180-1A), clone YEpAMS1 DNA.
ORGANISM Saccharomyces cerevisiae
Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
Endomycetales; Saccharomycetaceae.
REFERENCE 1 (bases 1 to 5189)
AUTHORS Yoshihisa,T. and Anraku,Y.
TITLE Nucleotide sequence of AMS1, the structure gene of vacuolar alpha-
mannosidase of Saccharomyces cerevisiae
JOURNAL Biochem. Biophys. Res. Commun. 163, 908-915 (1989)
STANDARD full staff_review
FEATURES from to/span description
pept 1054 4305 alpha-D-mannosidase (EC 3.2.1.24)
BASE COUNT 1645 a 941 c 1043 g 1560 t
ORIGIN
1 ctgcagacac tgttgaaaaa gttattgtta cttcgtctac tgctgctctg gtgacaccta
61 cagacatgaa caaaggagat ttggtgatca cggaggagag ttggaataag gatacatggg
121 acagttgtca ggccaacgcc gttgccgcat attgtggctc gaaaaagttt gctgaaaaaa
181 ctgcttggga atttcttaaa gaaaacaagt ctagtgtcaa attcacacta tccactatca
241 atccgggatt cgtttttggt cctcaaatgt ttgcagattc gctaaaacat ggcataaata
301 cctcctcagg gatcgtatct gagttaattc attccaaggt aggtggagaa ttttataatt
361 actgtggccc atttattgac gtgcgtgacg tttctaaagc ccacctagtt gcaattgaaa
421 aaccagaatg taccggccaa agattagtat tgagtgaagg tttattctgc tgtcaagaaa
481 tcgttgacat cttgaacgag gaattccctc aattaaaggg caagatagct acaggtgaac
541 ctgcgaccgg tccaagcttt ttagaaaaaa cgcttgcaag tgtgaaattc taagacaaaa
601 aaactactgg gattccagtt ttacaattta aaggattgca tagttgacac cgcggcgcaa
661 atgttagaag ttcaaaatga agcctaagta tcacgctaat tgaagttttt tttgatcact
721 ccaataggca aatctataga tatataaaaa atatagacaa gacttttttt tacattgcca
781 gttttctttt ttccttttta gtatctattc aaatgggcga ccctattgtc taatttcatt
841 agcttcatca caaaaagtgc cacgaattca tatagcgcga gggtaatccc taccctaact
901 cgtttaaggg agacatagaa tgaactccgc catcatgtgc tcaagactag acgaagctat
961 attagaagga caggaggagg ttttatttta accagttact tcatttctaa tatagagatt
1021 aaggctgcaa aagagtttca aaaataatta attatgtcat ctgaggatat catttatgat
1081 cctcagttca aacctgttca gggaatctac gagaatagat taagacaatt tattgatact
1141 ggtggggatt accatgactt aaatttaccc aagttttatg ataagaagcg catttcatta
1201 gatcatgatc acgttaaggt ttggtggtac caggtttctt ttgaacgtgg atcttcccct
1261 gtttcccctg ataagaggcc atcttggaaa tctattattg aacgtgacaa gaagggtgaa
1321 ttggagttta gagaagcgaa cataaatcag ccctttggac ccagttggtc taccacttgg
1381 ttcaaggtga aaatctcttt gcctgaagat tgggttaaat caaacgagca actacttttc
1441 caatgggatt gttctaacga aggaattgtc atagacccaa agactctaat ccctgtgact
1501 gctttttcag gtggtgaaag gactgaatat gttttaccga aaacatcgga tgggaaacat
1561 ttcttttaca tcgaagctgg taataatggt atgtttggct gcggtgcagg atccactata
1621 aatccaccag atgataatag attttttcat ttgagaaaag ctgatatcgt ttggcccgat
1681 ctagatgctc gtgcattgta tatcgatttt tggatgctag gggatgctgc aagagagcta
1741 cctggagact cctggcaaaa acaccaggca agacaactag gcaatgcagt tatgaatctt
1801 tttgacccga atgatcgttc aagtgttcgt aaatgtcgtg aattactaca aagggaatac
1861 tttgactcct ttttagaaag cagtaaggtt tacgaacaag gtgaatctca agttttaaca
1921 aatgtttatg gtatcggcaa ctgtcatata gatacagctt ggctatggcc attcgcagaa
1981 acaagaagga aaattgtgag atcttggtct tcccaatgta ctttaatgga tcgcttccca
2041 gagtataaat ttgttgcttc acaagcccaa caatttaaat ggttattaga agatcatccc
2101 gaatttttca ataaagtgtt aattccaaaa attcagcaat ctcagttttt tgctgttggt
2161 ggtacatggg ttgagaatga taccaatatt ccctcagggg aatcgctggc aaggcaattc
2221 ttttttggtc aaaggttttt cttgaagcat tttggtctca agtcaaagat attttggtta
2281 cctgacacct ttggttattc ttcgcaaatg ccacaacttt gccgcttatc tggtattgat
2341 aaatttttga ctcaaaagct ttcctggaat aacatcaaca gttttccgca tagcacgttt
2401 aactgggcag ggattgacgg ttctcaatta ttgactcaca tgcctcctgg taatacgtat
2461 acagcagatt cacacttcgg tgatgttttg cgtactgcca agcagaataa gacaccagaa
2521 tattacggct ccggtttaat gttgtacgga aagggtgatg gtggtggagg accaactgag
2581 gaaatgctgc aaaaaatgag gcgtattaga tccatgaata acagaaatgg taatgttatt
2641 ccaaagttac aagttggtat aacggttgat gaattttatg atgacatttt gaaaaggact
2701 aaccaaggcc atgatttacc cacatggagt ggtgaattgt atttcgaatt tcataggggg
2761 acatacacca gtcaagctca gaccaaaaaa ttaatgaggt tgtccgaaat taaactacat
2821 gatttggaat ggatagctgc caaaacatca gttttatatc ccgattcgta taaataccct
2881 tccaaacaga ttaacgaact ttgggagaat gttttgttat gtcaatttca cgatgttcta
2941 ccaggatcat gtattgaaat ggtatataag tatgaggctg tccctatgtt gcacaacgtt
3001 gtgaaagaat gtacttcctt aatagacaaa accgttcaat ttcttcagag tcagagtaag
3061 gcagatttgg ttgagatgag aacattaact tggtcaaaac cagaaaaggt ttctgaggag
3121 tgctcgttga acggtagtta cacgtcctct gtaaccgggt acgatgacta tatagtcctt
3181 gccaatggga agttgaaagt tatcatttgc aaaaagactg gggtgatcac aagcattacg
3241 gatgaaactt tgggggtaga atacctggac acggaacatg gcagaaacaa attgggcgcc
3301 aaccagtttg ttatttatga tgataaacct ttgggctggc aggcttggga tactgaactt
3361 tattccgtca accaatacaa gtatgtaaca aagccgaaga aagttcaact ttcctgcaac
3421 acaaaagaga aatgtgctgt tgaggctatt tttcaaattt ccgaaaaatg caaaatcaaa
3481 tcagtgatat cactcaatgc aactgcggtc actgatgcta aattaagtaa agtagacatt
3541 tctacgacgg tagaaaactg ggatgctaga aacaaatttt taaaagttga atttcccgtt
3601 aatattcgga acgattttgc ttcgtatgaa actcaattcg ggattaccaa aagaccaaca
3661 cattataata cctcatggga cgtcgctaaa tttgaggttt gccatcacaa atttgctgat
3721 tattctgaat acagcaaggg tgtttcaatt ttgaacgatt gcaaatatgg gttttccaca
3781 cacggtaact tgatgagatt atcattatta aggtcaccaa aggctccgga tgcgcatgct
3841 gatatgggta ctcatgagat aaaatatgct atctatccac atagaggagc actatcaagc
3901 gacactgtta agctcgctca cgaattcaat tattgcttca aatacaaact ccccaaagat
3961 attggcatga attttgatga catcataagc atttccggag atgagaatgt cattttgtct
4021 aacataaaaa gaggcgaaga tgatagtgct gtcaagtcca actattcatt aaatccaagg
4081 gatgaacaaa gtattgtggt aagggtttac gaatctcttg gcggggaatc ttttgcttca
4141 ctaaatacca cactaaattt gaagcgtatt gagaaggtcg ataacttgga gatgaaagtt
4201 tataagagtt tgacagcaac gcgagacgaa tcaaatcatg caataaacag aattcccatt
4261 aaattgagac cttttgagat tgcctcattc aggttgtatt tctgagtttg tctccctcac
4321 tgttttttac attcactcac tcccaaactt atgccatttc tatcatgata accaaaaata
4381 gtcttaacaa aatacatata agtaaataat gctaaataaa aaagcaaaaa aataaaattt
4441 atatcacact tgtaaaacct ttaaatgatc ttctttttta cctgttttat tcacttgttt
4501 ttttttttct gagttgttgc gccttggaag aatggaaaag caatagtttc agtgactata
4561 gtatacaatc aaaacacccg ccagttcaaa actattactt ggtcactaac ccccaagtca
4621 tctctagcaa tttaaatttc aattttaaga aaacctttat ttatccaacg tgaacaagta
4681 ctttcaagca cttctgccca ccgctatatc gtggaaaaat gtgtcaagct accaatggcc
4741 cgagtagagt tgtgactaaa agcataggaa attttcgaaa gacatctaca gttgcttccc
4801 tcttcacatc agggacatga cgtgaacaga atggcgataa tattctactc aatctcagga
4861 ctctctatat ttgaggttaa cgtttctgcg aagtacggcg atcatcttgg ctggatgcgc
4921 aaacattata tcaaaacagt gctggatgat acagaaaata ctgtgatatc tggatttgtt
4981 ggaagcttag tcatgaatat ccctcacgca acaacgatta atctaccaaa tactctcttt
5041 gcattgttgt ccatgattat gctgagagat tacgagtatt ttgagactat actagacaaa
5101 agaagcctgg cgagatttgt ttctaagtgc caacgacctg accgtggctc atttgtatct
5161 tgtttagact ataagacaaa ttgtggatc
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