GenBank-Updates@genbank.bio.net (08/18/90)
LOCUS ECOGUT 4770 bp ds-DNA BCT 18-AUG-1990
DEFINITION E.coli glucitol (gut) operon: glucitol-specific enzyme II (gutA),
and III (gutB), glucitol-6-phosphate dehydrogenase (gutD),
activator (gutM) and repressor (gutR) genes, complete cds.
ACCESSION J02708 M36721
KEYWORDS glucitol operon; glucitol-6-phosphate dehydrogenase;
glucitol-specific enzyme II; glucitol-specific enzyme III;
gut operon; gutA gene; gutB gene; gutD gene; gutM gene; gutR gene;
phosphotransferase.
SOURCE E.coli K12 DNA, clones pMYS[5,24,139].
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 3523)
AUTHORS Yamada,M. and Saier,M.H.Jr.
TITLE Glucitol-specific enzymes of the phosphotransferase system in
Escherichia coli: I. Nucleotide sequence of the gut operon
JOURNAL J. Biol. Chem. 262, 5455-5463 (1987)
STANDARD full staff_review
REFERENCE 2 (bases 2971 to 4770)
AUTHORS Yamada,M. and Saier,M.H.Jr.
TITLE Positive and negative regulators for glucitol (gut) operon
expression in Escherichia coli
JOURNAL J. Mol. Biol. 203, 569-583 (1988)
STANDARD simple staff_entry
COMMENT Draft entry and printed copy of sequence [1] kindly provided by
M.Yamada, 04-FEB-1987.
FEATURES from to/span description
pept 532 2052 glucitol-specific enzyme II (gutA)
pept 2063 2434 glucitol-specific enzyme III (gutB)
pept 2438 3217 glucitol-6-phosphate dehydrogenase (gutD)
pept 3323 3682 gut operon activator (gutM)
pept 3749 4522 gut operon repressor (gutR)
mRNA 491 > 3217 gutABD mRNA
mRNA 3562 > 4522 gutR mRNA
BASE COUNT 1118 a 1214 c 1250 g 1188 t
ORIGIN 1 bp upstream of RsaI site; about 58 min on K12 map.
1 taccgaatcc agacgcttcg cctgggagag aatttcctgc aactgctgac gatcgaaacc
61 gtgtttgttc accattttgt cgatgaactg ctgggcattc gggcttatta gcgaaatcgc
121 cgcccatctg catcacattg tgctgcggct caagcaggaa gccgccagac ggcggcttcc
181 ggtggtcgta tcagtctcag taggttttgg cttgctgcta caggcggcaa gcaacacaaa
241 aaggggaagc aatgttacat aacgacgctt gaacatgagg ggtccattta acagattcaa
301 ccaggggcaa gtatggtaaa gcatcacgcc ccgcacaagg aagcggtagt cactgcccga
361 tacggacttt acataactca actcattccc ctcgctatcc ttttattcaa actttcaaat
421 taaaatattt atctttcatt ttgcgatcaa aataacactt ttaaatcttt caatctgatt
481 agattaggtt gccgtttggt aataaaacaa taaatcctga aggagagaac aatgatagaa
541 accattactc atggtgcaga gtggtttatc gggctgttcc aaaagggcgg agaggtgttt
601 accgggatgg tgaccggcat tcttccgctg ttgattagcc tgctggttat catgaacgca
661 ctgattaatt ttatcggtca gcatcgtatt gaacgttttg ctcaacgttg cgccggtaac
721 cctgtttccc gttacctact gttaccgtgc attggcacgt ttgtcttttg caatccgatg
781 accctaagcc tgggtcgctt tatgccggaa aagtacaaac ccagctacta cgcggcggcc
841 tcttatagct gccactcaat gaatggcctc ttcccccata tcaaccctgg cgaactgttt
901 gtttatcttg gcattgccag cggtctgaca acgctgaacc tgccacttgg cccactggcg
961 gtgagttatc tgctggttgg tctggtcacc aatttcttcc gcggctgggt gaccgatctg
1021 accaccgcca tttttgagaa aaagatgggc attcaacttg aacaaaaagt tcagcctggc
1081 aggagcaaca tcatgacgca tattcggatc gaaaaaggaa cgggtggctg gggcggcccg
1141 cttgagctga aagccacgcc gggaaaaaaa atcgtctata tcaccgccgg tacccggcct
1201 gcgattgttg acaaactggc acagcttact ggctggcagg ctattgacgg atttaaagaa
1261 ggtgaacccg cggaggcgga aattggtgtc gcggtaatcg actgtggcgg cacattacgc
1321 tgcggcatct atccgaaacg acgtattccc accattaata tccactcgac gggcaagtcc
1381 ggtccgctgg cgcagtacat tgtggaagat atttatgtct ctggcgtaaa agaagaaaac
1441 atcactgtag taggtgatgc gacaccacaa ccctcttccg tgggccgtga ctatgacacc
1501 agtaagaaaa tcaccgaaca aagcgatggt ttactggcga aggtgggaat gggcatgggg
1561 tccaccgttg cggtgctgtt tcaatctggt cgtgacacca tcgacactgt attaaaaacc
1621 attctgccgt ttatggcatt cgtctcggcg ctcattggca tcattatggc ttctggcctt
1681 ggtgactgga ttgcccacgg tcttgctccg ctggcgagcc atccactggg tctggtcatg
1741 ctggcgctca tctgttcctt cccactgctt tcacctttcc tcggcccagg cgcagttatc
1801 gcacaggtta tcggcgtatt gattggcgtg cagattggtc tcggcaatat tccgccgcat
1861 ctggctttac cggcactgtt tgccatcaac gcgcaggcgg cctgcgactt catcccggtc
1921 ggtttgtcgc tggcggaagc ccgtcaggac acggttcgcg tcggtgtccc ttctgtactg
1981 gtgagccgct ttttaaccgg cgcgccgact gtactgatcg cctggtttgt ctccggtttt
2041 atctatcaat agaggctgaa acatgaccgt tatttatcag accaccatca cccgtatcgg
2101 cgcgagtgcc attgacgccc tcagtgacca gatgctcatc acctttcgtg aaggcgcgcc
2161 tgcggacctc gaagagtatt gcttcattca ttgccacggc gagttgaaag gtgcactcca
2221 tcccggtttg caattttcac tcgggcagca tcgctatccg gtgaccgctg ttggcagcgt
2281 ggcggaagac aaccttcgcg aactgggtca tgtcaccctg cgcttcgatg gtttaaacga
2341 agcggaattt ccgggcactg tccatgtggc aggccctgtc cccgacgata tcgcgccggg
2401 atcggttttg aagtttgaat ctgttaagga gtaaaaaatg aatcaggttg ccgttgtcat
2461 cggtggtggg caaaccttag gcgcgttcct gtgccacggt ctggctgccg aggggtatcg
2521 cgtcgcggtt gtcgatattc agagcgacaa agccgcaaat gtggcacaag aaattaacgc
2581 cgaatatggt gaaagtatgg cgtacggttt tggtgctgac gccactagcg agcaaagctg
2641 tctggcgctc tctcgtgggg tagatgaaat ctttggtcgc gtggatttgc tggtctacag
2701 cgccggaata gcaaaagcag cctttatcag cgacttccag ctcggcgatt ttgaccgttc
2761 gctacaggtg aatctggtgg gttatttcct gtgtgcgcgt gaattttcgc gtttgatgat
2821 ccgcgacggg attcaggggc gcattattca gatcaactcg aaatccggca aagtgggcag
2881 caaacacaac tctggctaca gcgcagcgaa atttggtggc gtcgggctga ctcaatcact
2941 ggcgctggat ctggcggagt acggcattac ggtgcattca ctgatgctcg gtaacctgct
3001 gaaatcgccg atgttccagt cactgttgcc acaatacgcg accaagctgg gtatcaaacc
3061 ggatcaagtc gagcagtatt acatcgacaa agtaccgctc aaacgcggct gcgattatca
3121 agatgtgctg aatatgctgc tgttctacgc cagtcctaag gcgtcgtact gcaccggaca
3181 gtcgatcaat gtcaccggcg gtcaggtgat gttctgatca acagcggaga tccattaagg
3241 atctccgctg agactataga atgcctgatg cgctacgctc atcaggcata caggacttcc
3301 gccactacat taaggaaaag ttatggtatc cgcactcatc accgtcgccg ttatcgcctg
3361 gtgtgcgcaa ctggccttag gcggctggca aatttctcgt tttaaccgtg ccttcgacac
3421 actatgccag caagggcggg ttggcgtggg ccgttccagc gggcgcttta aaccgcgggt
3481 cgtggtcgcc atcgcgctgg acgatcagca gcgcatcgtc gacaccttgt ttatgaaagg
3541 actgaccgtc ttcgcccgac cgcaaaaaat tcccgcaatt accggtatgc atgcgggtga
3601 tttacagccc gatgtgatct ttccccatga tccactatca cagaatgctc tatcattggc
3661 gcttaaactg aaacgtggat aatttcgttg tgaatgttac ttgcttgcga agttatcatt
3721 ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt cagcgtcagg ccgccattct
3781 ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa ttggcgcaat actttgacac
3841 cacaggcaca accattcgca aagatctggt cattctggaa catgccggaa ccgtcattcg
3901 tacttatggc ggagtggtgt tgaataaaga ggaatccgat ccgcctatcg atcataaaac
3961 actcatcaac acccacaaga aagagctgat tgcagaagct gccgttagtt ttatccatga
4021 tggcgattcg atcattcttg atgctggcag taccgttttg cagatggttc ccctgctctc
4081 gcgctttaat aacatcacgg tgatgaccaa cagcctgcat atcgtcaatg cgctatccga
4141 actggataac gaacaaacta tcctgatgcc aggcggaacg tttcgcaaaa aatcggcctc
4201 atttcacggg caactggcag agaatgcctt cgagcatttc accttcgata aattgtttat
4261 gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacc tttaacgagg tttataccgt
4321 cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg atggcggact catcaaagtt
4381 tggccgtaaa agccccaacg tagtttgcag tcttgaaagc gtcgataagc tgattaccga
4441 cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag aaagggatcg atgtgatcat
4501 aaccggagag agcaatgagt gaagcactac tgaacgcggg acgtcagacg ttaatgctgg
4561 agttgcagga agcaagccgt ttaccggaac gtctgggcga tgattttgtt cgcgccgcca
4621 atatcatcct gcactgtgaa ggcaaagtgg tggtttcggg aattggcaaa tcgggccaca
4681 ttggtaagaa aatcgccgca acgcttgcca gtaccggcac tccggctttt tttgtccatc
4741 cggcagaagc gctgcacggc gatctgggga
//