GenBank-Updates@genbank.bio.net (08/18/90)
LOCUS BSUSACXY 2984 bp ds-DNA BCT 18-AUG-1990
DEFINITION B.subtilis positive levansucrase and sucrase synthesis regulatory
protein (sacY) and negative regulatory protein of sacY (sacX)
genes, complete cds.
ACCESSION M29333
KEYWORDS levansucrase regulatory protein; sucrase regulatory protein.
SOURCE B.subtilis (strain QB1072) DNA, clones pSL[42,43].
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes;
Endospore-forming rods and cocci; Bacillaceae.
REFERENCE 1 (bases 1 to 2984)
AUTHORS Zukowski,M.M., Miller,L., Cogswell,P., Chen,K., Aymerich,S. and
Steinmetz,M.
TITLE Nucleotide sequence of the sacS locus of Bacillus subtilis reveals
the presence of two regulatory genes
JOURNAL Gene 90, 153-155 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by M.M.Zukowski, 23-OCT-1989.
FEATURES from to/span description
pept 124 1503 regulatory protein sacX (ttg start codon,
put.)
pept 1557 2399 regulatory protein sacY
ORF > 2984 2451 (c) ORF D1 (AA at 2984)
signal 2407 2441 pot. transcription termination signal
binding 106 114 sacX ribosome binding site (put.)
binding 1539 1545 sacY ribosome binding site (put.)
BASE COUNT 803 a 662 c 658 g 861 t
ORIGIN
1 gtcgacgcgg gcatttttgt cattttcagc cggtatttct cacttttttc attttgattg
61 tcaacttttc atttgatacg atgaacaagc cgacttttct tcagcaaagg aggttttaat
121 tgcttgcata aagagattgc aaaagaatta ctgctgctcg caggaggaaa aaacaatatt
181 atcagcatca gccattgtac gacccgtctt cgttttgatg taaaggacga gacgaaaatt
241 gatatacatg ccattgagaa cctgcaaggt gtgcagggca cctttttccg ctatgggctt
301 ttccagatta tcttcggagc aggcgtcgtc aacaaaatat ataaagaagt cgttcatgta
361 tgggagaccg cgccttctga agagcctgtc caccagaaaa aagccagccg gaagctgaac
421 cctgccgcag cttttgcgaa aacactgtcc gatatttttg tgccgattat tccggcaata
481 acagcaagcg gcctgctcat ggggctgatc ggcatgatta aggtgtttca ttggttcgct
541 gccggcagcc catggattaa aatgctcgac ctcgtgtcca gcacagcctt tattttactg
601 ccgattttag tcggcttcag tgctgcacgg caatttggca gcaacccata cctaggggca
661 gtgattgcag ggcttttgac gcatcctgat ctgctggacc cgtctatgct cggcagcaaa
721 accccgtctt ctttagacat atgggggctt catatcccaa tgatgggcta tcagggttcc
781 atgataccga ttcttctttc cgtttttgtc atgagcaaaa ttgagaagct attaaaatcg
841 attgtgccga agtcgcttga tgtcgtgatc attcccttta ttacggtcat ggtgacaggg
901 tgcctcgcgc tgattgtgat gaatcccgcc gcgtccatca tcggccagat catgacacaa
961 tcgatcgtct atatttatga tcacgccggc attgctgccg gagctctttt cggcggcatc
1021 tactccacca tcgtcctatc tggattgcat catagctttt atgcaattga ggcaacgctg
1081 cttgctaatc cgcatgtcgg tgtcaacttt ttagtgccga tatggtcgat ggcgaatgtg
1141 gcccaaggcg gagcggggct tgcggtattc ctcaaaacga aacaatcaag cctgaagaaa
1201 attgcgctcc ccgcttctct gaccgcgttt ttaggcattg ttgagccgat tgtattcggg
1261 gtaaatctca aattgatccg tccctttatc ggagcagcca tcggcggtgc tattggcgga
1321 gcatacgtgg ttgcggtaca agttgtggca aattcctacg gactgacagg cattccgatg
1381 atttcaatcg tgctgccgtt tggcgccgct aattttgttc attatatgat cggtttcttg
1441 atcgcagccg tctctgcttt tatagctaca ttgtttctcg ggtttaaaga agagacagaa
1501 taactggatt tattcgattt cattcataaa acgggggatg aaaggacaaa aaagctatga
1561 aaattaaaag aatcttaaat cataatgcta tcgtcgtaaa ggatcaaaat gaagagaaga
1621 ttctcttggg tgcaggaatt gcgtttaaca aaaagaagaa tgatattgtc gatccgtcaa
1681 aaatagaaaa aacctttatc agaaaagata cacctgacta taagcagttc gaagagattt
1741 tagaaacatt gcctgaagac cacattcaga tttctgagca aattatctct catgccgaaa
1801 aagagctgaa catcaaaatc aacgagcgca ttcatgtcgc tttttcagac catctttctt
1861 ttgcaattga acgcctgagc aatgggatgg ttatcaaaaa tccgctgctg aatgaaatca
1921 aagtccttta tccaaaggag ttccagatcg gcttatgggc cagagcactg attaaagata
1981 aactggggat tcacattcct gatgatgaaa tcggcaatat cgccatgcat atccacacag
2041 caagaaacaa tgccggcgat atgacacaaa cgcttgatat tacaacaatg atccgtgata
2101 ttatcgagat tatcgaaatt caactgtcta ttaatatcgt tgaagatacc atctcttatg
2161 aaaggctcgt gacccatctc cgctttgcca ttcagcatat caaagcaggc gaatccattt
2221 acgagctgga cgcagaaatg attgacatca ttaaagagaa gtttaaggat gccttcctgt
2281 gtgccctaag catcggcacc tttgtgaaga aggaatacgg ctttgagttt cctgaaaaag
2341 aattgtgcta catcgccatg catattcagc ggttctacca acggtcagtc gcacgctgag
2401 acaaacaaaa aacgcttttg atcatctcaa aagcgttttt ttcatctgat ttattggttg
2461 atcgccggat tcccaatcgt attccacttc gtataggaca tgtaatggaa atactcatcg
2521 cgatacggat gttttgtatt ggagttccaa ggcttttctc cgccgcagaa gtgcacgatt
2581 gccgggtttt ctcttgtttc attatattgc ttccgcccta acagagtcga tggggttttc
2641 agctttaaca tgatataggt ttgagcgttc cagcgcggat gaagttcata ccactgatca
2701 tacaagatcg cattcagtgc atcttggtca tgcagcacaa gaaagtcttc gtctgggtgt
2761 tcattgataa agttgatgac tttttctgtg atgttttgct ttctccaaga ctcaaaatca
2821 ataatcatga tacctgagtt aaaatacttc cctgtatcag tgacgttcat ttctttcagg
2881 cgttcatgct gccccgcatc ctcaacagcg gctacggtgt atggcgcaat gtctaagtcc
2941 catagcttag aaatatcctc taggacaagc gcatcacaat cgat
//