GenBank-Updates@genbank.bio.net (08/21/90)
LOCUS CHKTEN 6100 bp ss-mRNA VRT 21-AUG-1990
DEFINITION Chicken alternatively spliced tenascin 190, 200 and 230 kd
variants mRNA, complete cds.
ACCESSION M23121
KEYWORDS alternative splicing; cytotactin;
extracellular matrix glycoprotein; hexabrachion;
myotendinous antigen; tenascin.
SOURCE Chicken embryo (10 or 11 day old) and skin fibroblast, cDNA to
mRNA, clones lambda cTn[190,200,230].
ORGANISM Gallus gallus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Aves;
Neornithes; Neognathae; Galliformes; Phasianidae.
REFERENCE 1 (sites)
AUTHORS Spring,J., Beck,K. and Chiquet-Ehrismann,R.
TITLE Two contrary functions of tenascin: Dissection of the active sites
by recombinant tenascin fragments
JOURNAL Cell 59, 325-334 (1989)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 6100; for [1])
AUTHORS Spring,J.
JOURNAL Unpublished (1989) Postfach 2543, CH-4002 Basel, Switzerland
STANDARD full staff_review
COMMENT Draft entry computer-readable sequence for [1],[2] kindly submitted
by J.Spring, 14-MAR-1989.
FEATURES from to/span description
pept 235 5661 230 kd tenascin precursor
sigp 235 333 230 kd tenascin signal peptide
matp 334 5658 230 kd tenascin
pept 235 3907 200 kd tenascin precursor
4190 5661 200 kd tenascin precursor
sigp 234 333 200 kd tenascin signal peptide
matp 334 3907 200 kd tenascin
4190 5658 200 kd tenascin
pept 235 3361 190 kd tenascin precursor
4190 5661 190 kd tenascin precursor
sigp 235 333 190 kd tenascin signal peptide
matp 334 3361 190 kd tenascin
4190 5658 190 kd tenascin
binding 5428 5469 calcium binding site
BASE COUNT 1567 a 1522 c 1709 g 1302 t
ORIGIN Unreported.
1 ggggtttgac aggacggcga ggaatccggg agccgacagc tggctgcagt acctctgctt
61 cgtggaggct gcccgtggca ggatctgatc cgtcagccca cacgagaata agcgtgccaa
121 gaaaggaaag gaaactcaac ttagtttgaa ctggctctca aatttctcct ccagtctaca
181 aaggccaaac aaatataaga ctccatcagc tttgaaaagg aactgagcac tacaatggga
241 ctcccttccc aggttttggc ctgtgccatc ttaggtttgc tgtaccagca tgccagtggt
301 gggctcatca agcgaattat ccggcagaag cgggagactg ggctcaatgt gaccttacca
361 gaggataatc agcctgtggt tttcaatcat gtctacaaca tcaagctgcc tgttggctcc
421 ctttgctctg tggacctgga cacagcaagc ggggacgcag acctgaaggc agaaattgag
481 cctgtcaaga attacgagga gcatacggtg aatgagggga accagattgt cttcacgcac
541 cgcatcaaca ttccccgccg ggcctgtggc tgtgcggctg ccccagacat caaggacctg
601 ctgagcagac tggaggagct ggaggggctg gtatcctccc tccgggagca gtgtgccagc
661 ggggctggat gctgtcctaa ttcccagaca gcagaaggtc gcctggacac ggccccctat
721 tgcagtgggc acggcaacta cagcaccgag atctgtggct gcgtgtgcga gccaggctgg
781 aaaggcccca actgctccga accggcctgc ccacgcaact gcctcaaccg cggcctctgc
841 gtgcggggca aatgcatctg cgaggagggc tttaccggcg aggactgcag ccaggctgcc
901 tgcccgtctg actgcaacga ccaaggcaag tgtgtggatg gggtgtgcgt ctgcttcgag
961 ggctacacgg gcccggactg cggcgaggag ctctgccccc acgggtgtgg cattcacggg
1021 cgctgtgtgg gtggacgctg tgtgtgccac gagggcttca ctggcgagga ctgcaatgag
1081 cccctgtgcc ccaacaactg tcacaaccgc gggcgctgtg tggacaacga gtgcgtctgc
1141 gatgagggct acacgggaga ggactgcggc gagctgattt gccccaatga ctgctttgac
1201 cgtgggcgct gtatcaacgg gacctgcttc tgcgaggagg gctacactgg agaggactgc
1261 ggcgagctga cctgccccaa caactgcaac ggcaacgggc gctgcgagaa cgggctgtgt
1321 gtgtgccatg agggcttcgt gggggatgac tgcagccaga agaggtgccc gaaggactgc
1381 aataaccgcg ggcactgcgt ggatgggcgc tgtgtgtgcc atgaggggta cctgggggag
1441 gactgtgggg agctgcggtg ccccaacgac tgccacaacc gcgggcgctg catcaatggg
1501 cagtgtgtgt gtgatgaggg attcattggg gaggactgtg gagagctgcg gtgccccaac
1561 gactgccaca accgcgggcg ctgcgtcaat gggcagtgcg agtgccacga gggattcatc
1621 ggggaggact gcggggagct gcggtgtccc aacgactgca acagccatgg gcgctgtgtc
1681 aatgggcagt gcgtgtgtga tgaggggtac acaggggagg actgcgggga gttgcggtgc
1741 cccaacgact gccacaaccg cgggcgctgc gtggagggac gctgtgtgtg tgacaacggc
1801 ttcatggggg aggactgcgg ggagctgtcc tgtcccaatg actgccacca gcacgggcgc
1861 tgcgtcgatg ggcgctgcgt gtgccacgag ggcttcactg gggaagactg ccgggaacgg
1921 tcctgcccca atgactgcaa caacgtgggc cgctgtgtcg agggacggtg tgtctgtgag
1981 gaaggttaca tggggatcga ctgttctgat gtgtctcctc caacggagct gactgtaacg
2041 aatgtaacag ataaaacggt aaatctggaa tggaagcatg agaatctcgt caatgagtac
2101 cttgtcacct atgtccctac cagcagtggt ggcttagatc tacagttcac cgtaccagga
2161 aaccagacat ctgccactat tcatgagctg gagcctggtg tggaatactt catccgtgtc
2221 tttgcaatcc ttaaaaacaa gaaaagtatt ccagtcagtg ccagagtagc gacatatttg
2281 cctgctccag aaggtctgaa attcaaatct gttagagaaa cgtctgtcca ggtggaatgg
2341 gatcctctga gcatttcctt tgatggctgg gagctggtct ttcgtaatat gcagaaaaag
2401 gatgataatg gagacataac cagcagcttg aaaaggccgg agacatcata tatgcagcca
2461 ggattggcac caggacaaca gtataatgta tcccttcata tagtgaaaaa caataccaga
2521 ggaccagggc tatcccgagt gataaccaca aaactcgatg cccctagcca gattgaggcg
2581 aaagatgtca cagacaccac agctctgatc acatggtcca aacccttggc tgaaattgaa
2641 ggcatagagc tcacatatgg ccccaaggat gttccagggg acaggactac cattgacctc
2701 tctgaggatg aaaaccaata ttctattgga aacctgaggc cacacacaga atatgaagtg
2761 acactcattt ctcggcgagg ggacatggag agtgaccctg caaaagaagt ctttgtcaca
2821 gacttggatg ctccacgaaa cctgaagcga gtgtcacaga cagacaacag cattactttg
2881 gagtggaaga acagccatgc aaatattgat aattaccgaa ttaagtttgc tcccatttct
2941 ggtggagacc acactgagct gacagtgcca aagggcaacc aagcaacaac cagagctaca
3001 ctcacaggtt tgagacctgg aactgaatat ggcattggag tgacagcagt gagacaggac
3061 agggaaagtg ctcctgctac cattaatgct ggcactgatc ttgataaccc caaggacttg
3121 gaagtcagtg accccactga aaccaccctg tcccttcgct ggagaagacc agtggccaaa
3181 tttgatcgtt accgcctcac ttacgttagc ccctctggaa agaagaacga aatggagatc
3241 cctgtggaca gcacctcttt tatcctgaga ggattagacg cagggacgga gtacaccatc
3301 agtctagtgg cagagaaagg cagacacaaa agcaaaccca caaccatcaa gggttcgact
3361 gaggaagaac ctgagcttgg aaacttatca gtgtcagaga ctggctggga tggtttccag
3421 ctcacctgga cagcagccga cggggcctat gagaactttg tcattcaggt gcagcagtct
3481 gacaatccag aagaaacctg gaacattaca gtccccggcg gacagcactc tgtgaacgtt
3541 acaggcctca aggccaacac accttataac gtcacacttt acggtgtgat tcgaggctac
3601 agaaccaaac ccctttatgt tgaaaccacg acaggagcac accccgaagt tggtgagcta
3661 accgtttccg acattactcc tgaaagcttc aacctttctt ggacgaccac caacggggac
3721 tttgacgcct ttactattga aattattgat tctaacaggt tgctggagcc catggagttc
3781 aacatctcag gcaattcaag aacagctcat atctcagggc tttcccccag cactgatttt
3841 attgtctacc tctatgggat ctctcatggt ttccgcacac aggcaataag tgctgcggct
3901 acaacagagg cagaacccga ggtggacaac cttctggttt cagatgctac cccagacggc
3961 ttccgtctgt cctggactgc agatgatggg gttttcgaca gttttgttct aaaaatcagg
4021 gataccaaaa ggaaatctga tccactggaa ctcatagtac caggccatga gcgcacccat
4081 gatataacag ggctgaaaga gggcactgag tatgaaattg agctctatgg agttagcagt
4141 ggacggcgct cccaacccat aaattcagta gcaaccacag ttgtgggatc tcccaaggga
4201 atctctttct cggacatcac agaaaactct gctacagtca gctggacacc cccccgcagc
4261 cgtgtggata gctacagggt ctcctatgtc cccatcacag gcggcactcc caatgttgtt
4321 acagttgatg gaagcaagac aaggacaaag ctggtgaagt tagtcccagg tgtagactac
4381 aacgttaata tcatctctgt gaaaggcttt gaagaaagcg aacccatttc tggaattctg
4441 aaaacagctc tggacagccc gtcaggactg gtagtgatga acattacaga ctcggaggct
4501 ctggcaacct ggcagcctgc aattgcagct gtggataatt acattgtctc ctactcttct
4561 gaggatgagc cagaagttac acagatggta tcaggaaaca cagtggagta cgacctgaat
4621 ggccttcgac ctgcgacaga gtacaccctg agggtgcatg cagtgaagga tgcgcagaag
4681 agcgagaccc tctccaccca gttcactaca ggactcgatg ctccaaaaga tttaagtgct
4741 accgaggttc agtcagaaac agctgtgata acgtggaggc ctccacgtgc tcctgtcact
4801 gattacctcc tgacctacga gtccattgat ggcagagtca aggaagtcat cctagaccct
4861 gagacgacct cctacaccct gacagagctg agcccatcca ctcaatacac agtgaaactt
4921 caggcactga gcagatctat gaggagcaaa atgatccaga ctgttttcac cacaactggt
4981 cttctttatc cttatcctaa agactgctcc caagctctcc tgaatggaga ggtcacctct
5041 gggctctaca ctatttatct gaatggagac aggacacagc ctctgcaagt cttctgtgac
5101 atggctgaag atggaggcgg atggattgtg ttcctgaggc gtcaaaatgg aaaggaagat
5161 ttctacagga actggaagaa ttacgtggcc ggctttggag atcccaagga tgaattctgg
5221 ataggtctgg agaacctcca caaaatcagc tctcaggggc agtacgagct gcgtgtggat
5281 ctgagagaca gaggtgagac agcctatgct gtgtacgaca agttcagcgt tggagatgcc
5341 aagacccggt accggctgag ggtggatggc tacagtggca cagcaggtga ctccatgacc
5401 taccataatg gaagatcctt ctccactttt gacaaggaca atgattctgc tatcaccaac
5461 tgtgctttgt catacaaggg tgctttctgg tacaagaatt gtcaccgagt caatctgatg
5521 ggcagatatg gtgacaacaa ccacagtcag ggtgttaatt ggttccactg gaagggccac
5581 gaatactcca tccagtttgc agagatgaaa ctgagaccct ccagctttcg gaatctggaa
5641 ggaagacgaa agcgagcata aagccttggg atggtgaaag ggctacgggc agggcaacat
5701 ggggagggac agagagcggg gggcatggga ggatctctgg catcactggg gttatgggtg
5761 tgaggagctg gtagtcgtac caaagcatcg caacccttgg cacaagagcc caaacaacga
5821 gccttacgtg tcccagcaat tccacagagc agctccagct ctgcccactg ctgatgtcct
5881 tcacgccaaa gacaacgatc tcaagggttg tatgctgttt tcttcatttt tcttttctca
5941 gcctctggga tgaagttctt ccacggtctg actatctctt gggtggccca gggtagggga
6001 gggagaacag ctttgtatat tggtagtttg ttttagaacc agccatattt tttacacaca
6061 gaaatgtgta tgattaatta tcattattat tattattaga
//