GenBank-Updates@genbank.bio.net (08/18/90)
LOCUS HUMIGHHSG3 893 bp ds-DNA PRI 18-AUG-1990 DEFINITION Human mutant germline Ig H-chain DNA with a deletion of IgG1, IgG2, IgG4 and IgA1, clone pBR0.9EZZ. ACCESSION M25382 J03079 M29704 KEYWORDS alpha-immunoglobulin; constant region; gamma-immunoglobulin; immunoglobulin heavy chain; minisatellite repeat; switch region. SOURCE Human (TOU and HASS families) cell line EZZ DNA, clone pBR0.9EZZ. ORGANISM Homo sapiens Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae. REFERENCE 1 (bases 1 to 893) AUTHORS Keyeux,G., Lefranc,G. and Lefranc,M.-P. TITLE A multigene deletion in the human IGH constant region locus involves highly homologous hot spots of recombination JOURNAL Genomics 5, 431-441 (1989) STANDARD full staff_entry COMMENT Draft entry and computer-readable sequence for [1] kindly provided by M.-P.Lefranc, 08-JUN-1989. FEATURES from to/span description site 64 71 chi sequence site 146 155 core of hypervariable minisatellite site 165 174 core of hypervariable minisatellite site 215 219 switch sequence repeat tetranucleotide rpt 847 854 direct repeat copy A rpt 857 864 direct repeat copy B BASE COUNT 215 a 213 c 216 g 249 t ORIGIN 1 bp upstream of BamHI site; chromosome 14q32. 1 ggatccacca ttctggggtc tggaggacag tggccccctt cccacagctc cgctaggcag 61 tgccctggtg gggacattat atgggggttc aaccacattc tctttggcac tgctctagta 121 gaggttctct gtgagggctc catgctggca ggaggcttct gtctgggcag ctaggctttc 181 tcatacattt ctgaaatcta gagggaagat gccaagcttc cttcactctt gcattctggg 241 ggcctacagg cttaacacca gagtggaagt caccaaggct tatagtggct tgcactctcc 301 aaagcagcag cctaagctgt acttggggcc ctttgagcca aggctggagc cagaggaacc 361 aggatgtgga gagcagtgtc ccaaggctgc acaggcagag tggccatggc atggccacaa 421 aacatctttc ctcttggcct ctgggctgtg atgggaaaga ccacctcaga gatttccaaa 481 atgccttcaa ggcctttttc cctttgttct ggatatgagc acttagctcc cttttagtta 541 tgctaatctc tctagcaact ggctgctcca tagcctactt atatttgtct cctgaaaata 601 ccttttcttt tctaccacat ggccaggctg tgaattttcc acatttttta tgctctgctt 661 ccctttgttg atgaaaaaag ccaaactcca taaaacactt gaaaaattga ttctgagcca 721 aatatgagag ccatgaactg tggcacagcc tcaaaaggtc ctgagaacac ctgtgcaagg 781 tggttgggtt gcggcctgct tttatgtttt agggagacat atcaatcaat acatgtaaag 841 tatacattgg tttgatttgg tttggaaaag caggacaatt caaatgggaa ttc //
GenBank-Updates@genbank.bio.net (08/21/90)
LOCUS HUMIGHHSG3 893 bp ds-DNA PRI 21-AUG-1990 DEFINITION Human mutant germline Ig H-chain DNA with a deletion of IgG1, IgG2, IgG4 and IgA1, clone pBR0.9EZZ. ACCESSION M25382 J03079 M29704 KEYWORDS alpha-immunoglobulin; constant region; gamma-immunoglobulin; immunoglobulin heavy chain; minisatellite repeat; switch region. SOURCE Human (TOU and HASS families) cell line EZZ DNA, clone pBR0.9EZZ. ORGANISM Homo sapiens Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia; Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae. REFERENCE 1 (bases 1 to 893) AUTHORS Keyeux,G., Lefranc,G. and Lefranc,M.-P. TITLE A multigene deletion in the human IGH constant region locus involves highly homologous hot spots of recombination JOURNAL Genomics 5, 431-441 (1989) STANDARD full staff_entry COMMENT Draft entry and computer-readable sequence for [1] kindly provided by M.-P.Lefranc, 08-JUN-1989. FEATURES from to/span description site 64 71 chi sequence site 146 155 core of hypervariable minisatellite site 165 174 core of hypervariable minisatellite site 215 219 switch sequence repeat tetranucleotide rpt 847 854 direct repeat copy A rpt 857 864 direct repeat copy B BASE COUNT 215 a 213 c 216 g 249 t ORIGIN 1 bp upstream of BamHI site; chromosome 14q32. 1 ggatccacca ttctggggtc tggaggacag tggccccctt cccacagctc cgctaggcag 61 tgccctggtg gggacattat atgggggttc aaccacattc tctttggcac tgctctagta 121 gaggttctct gtgagggctc catgctggca ggaggcttct gtctgggcag ctaggctttc 181 tcatacattt ctgaaatcta gagggaagat gccaagcttc cttcactctt gcattctggg 241 ggcctacagg cttaacacca gagtggaagt caccaaggct tatagtggct tgcactctcc 301 aaagcagcag cctaagctgt acttggggcc ctttgagcca aggctggagc cagaggaacc 361 aggatgtgga gagcagtgtc ccaaggctgc acaggcagag tggccatggc atggccacaa 421 aacatctttc ctcttggcct ctgggctgtg atgggaaaga ccacctcaga gatttccaaa 481 atgccttcaa ggcctttttc cctttgttct ggatatgagc acttagctcc cttttagtta 541 tgctaatctc tctagcaact ggctgctcca tagcctactt atatttgtct cctgaaaata 601 ccttttcttt tctaccacat ggccaggctg tgaattttcc acatttttta tgctctgctt 661 ccctttgttg atgaaaaaag ccaaactcca taaaacactt gaaaaattga ttctgagcca 721 aatatgagag ccatgaactg tggcacagcc tcaaaaggtc ctgagaacac ctgtgcaagg 781 tggttgggtt gcggcctgct tttatgtttt agggagacat atcaatcaat acatgtaaag 841 tatacattgg tttgatttgg tttggaaaag caggacaatt caaatgggaa ttc //