GenBank-Updates@genbank.bio.net (08/22/90)
LOCUS SYNIGHAC 465 bp ss-mRNA SYN 22-AUG-1990
DEFINITION Mouse heavy-chain and kappa-chain variable regions/human constant
region E6 anti-transferrin receptor Ig chimeric mRNA (V-kappa:
mouse/C-region:human).
ACCESSION M29533
KEYWORDS constant region; immunoglobulin heavy chain;
immunoglobulin light chain; kappa-immunoglobulin; processed gene;
variable region.
SOURCE Mouse SP2/O-derived hybridoma E6, cDNA to mRNA; fused to human IgG1
C-region gene, clone pUC-Ig10.
ORGANISM Artificial gene
Artificial sequences; Genes.
REFERENCE 1 (bases 1 to 465)
AUTHORS Hoogenboom,H.R., Raus,J.C.M. and Volckaert,G.
TITLE Cloning and expression of a chimeric antibody directed against the
human transferrin receptor
JOURNAL J. Immunol. 144, 3211-3217 (1989)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by H.R.Hoogenboom, 27-OCT-1989.
FEATURES from to/span description
pept 28 > 465 chimeric E6 anti-transferrin receptor Ig
V-region precursor
sigp 28 84 chimeric E6 anti-transferrin receptor Ig
V-region signal peptide
matp 85 > 465 chimeric E6 anti-transferrin receptor Ig
V-region
recomb 429 430 mouse V-region DNA end/human C-region DNA
start
BASE COUNT 119 a 131 c 98 g 117 t
ORIGIN
1 cagtcactga aaacattgac tctaatcatg gaatgtaact ggatacttcc ttttattctg
61 tcggtaactt caggggtcta ctcagaggtt cagctccagc agtctgggac tgtactggca
121 aggcctgggg cttcagtgaa gatgtcctgc aaggcttctg gctacaccat ttccagctac
181 tggatgcact ggataaaaca gaggcctgga cagggtctgg actggattgt cgctattgat
241 cctcgaaata gtgatactat ttacaacccg caattcaaac acaaggccaa actgactgca
301 gtcacctcca ccagcactgc ctacatggaa ctcaacagcc tgacaaatga ggactctgcg
361 gtctattact gtacccctct ttattacttt gactcttggg gccaaggcac cactctcaca
421 gtctcctcag ccaaaacgac acccccatct gtctatccac tggcc
//