GenBank-Updates@genbank.bio.net (08/22/90)
LOCUS SYNIGHAD 475 bp ss-mRNA SYN 22-AUG-1990
DEFINITION Mouse heavy-chain and lambda-chain variable regions/human constant
region E6 anti-transferrin receptor Ig chimeric mRNA (V-lambda:
mouse/C-region:human).
ACCESSION M29534
KEYWORDS constant region; immunoglobulin heavy chain;
immunoglobulin light chain; lambda-immunoglobulin; processed gene;
variable region.
SOURCE Mouse SP2/O-derived hybridoma E6, cDNA to mRNA; fused to human IgG1
C-region gene, clone pGV-3B4.
ORGANISM Artificial gene
Artificial sequences; Genes.
REFERENCE 1 (bases 1 to 475)
AUTHORS Hoogenboom,H.R., Raus,J.C.M. and Volckaert,G.
TITLE Cloning and expression of a chimeric antibody directed against the
human transferrin receptor
JOURNAL J. Immunol. 144, 3211-3217 (1989)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by H.R.Hoogenboom, 27-OCT-1989.
FEATURES from to/span description
pept 74 > 475 chimeric E6 anti-transferrin receptor Ig
V-region precursor
sigp 74 140 chimeric E6 anti-transferrin receptor Ig
V-region signal peptide
matp 141 > 475 chimeric E6 anti-transferrin receptor Ig
V-region
recomb 464 465 mouse V-region DNA end/human C-region DNA
start
BASE COUNT 135 a 100 c 118 g 121 t 1 others
ORIGIN
1 cagagctaga ccgatccttg gtgtgtgtta actcaggaag aaaaagacga cataaaacac
61 agcaggactc agcatggaca tgaggacccc tgctcagttt cttggaatct tgttgctctg
121 gtttccaggt atcaaatgtg acatcaagat gacccagtct ccatcttcca tgtatgcatc
181 tctaggagag agagtcactt tcacttgcaa ggcgagtcag gacattaata actatttatg
241 ctggttccag cagaaaccag ggaaatctcc taagaccctg atctatcgtg caaacagact
301 ggtagatggg gtcccatcaa ggttcagtgg cagtggatct ggacaagatt attctctcac
361 cattagcagc ctggagtatg aagatatggg aatttattat tgtctacagt atgatgagtt
421 tccgtacacg ttcggagggg ggaccaagct ggaaataaaa cgggctgatg ctgcn
//