GenBank-Updates@genbank.bio.net (08/24/90)
LOCUS CCRTRPFBA 3562 bp ds-DNA BCT 24-AUG-1990
DEFINITION C.crescentus trpFBA operon genes encoding tryptophan biosynthetic
enzymes trpA, trpB and trpF, complete cds.
ACCESSION M19129
KEYWORDS tryptophan biosynthetic enzyme; tryptophan synthase.
SOURCE C.crescentus DNA.
ORGANISM Caulobacter crescentus
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Budding and/or appendaged bacteria; Prosthecate bacteria.
REFERENCE 1 (bases 1 to 3562)
AUTHORS Ross,C.M. and Winkler,M.E.
TITLE Structure of the Caulobacter crescentus trpFBA operon
JOURNAL J. Bacteriol. 170, 757-768 (1988)
STANDARD full staff_review
COMMENT Draft entry and computer-readable copy of sequence [1] kindly
provided by M.E.Winkler, 18-NOV-1989.
FEATURES from to/span description
pept 181 450 usg protein
pept 500 1159 trpF protein
pept 1182 2402 tryptophan synthase B protein (trpB; gtg start
codon; EC 4.2.1.20)
pept 2415 3242 tryptophan synthase A protein (trpA; gtg start
codon; EC 4.2.1.20)
mRNA 151 > 3242 trpFBA operon mRNA (alt.)
mRNA 152 > 3242 trpFBA operon mRNA (alt.)
BASE COUNT 588 a 1214 c 1183 g 577 t
ORIGIN
1 cagtccgccc ccagaggggg cggaaggaac gcgcgaacct tcttccccct ccgggggagg
61 agcgcgcagc gcggaggggg tgagagacca agagcgcaac cccgcgacaa cgcgccgctt
121 gagcgactcg ccaagtcggc caaatcttct ggtgtcggaa cgtttgtcgg aggctcttcg
181 atggcgagta aggcgttcga gctgcagatg atgggctacg gcctgaccac ggccgagatt
241 cactaccaca tgcccgacca ccccggcctg ctgcagctct atgtctggca ggagtatgat
301 ctggcgccga aattcccgac cctgaagggg ttcctggatt tctgggccaa ggagctggac
361 ggggtgctgc attcggtgcg cgtggcgcac aaccggctga tcagtccccg ggaatggcgg
421 gtcgtgaacg gcgtcttcac gctgcaatga gcatcgccgg ggttgcgcgg cggcttcccc
481 gcccgtaact cccccggcga tgagcatttc cgcccatggg gccaagatct gcggactgtc
541 gacgcccgag acggtcaagg ccgcctttga cggcgccgcc gcgttcctgg gctttgtctt
601 cttcgacaag agcccgcgca atgtcgcgcc cgaagtcgcc gcgcgccttg tggagccggt
661 tcgcggccgg ggcgtacaga cggtcgccgt caccgtcgat ccggacgacg ccctgatcga
721 ccgcctgatg gcgacgatgc ggcccgacct gatccaggtg cacggcaagg agacgccctc
781 gcgggttcgc gagatcgccg cccgcgccgg cgtcggggtg atcaaggcct tttccgtctc
841 ctcatcggcg gatgtcgatc aggccgcggc ctttgacggc gtggcccaac acctgatgtt
901 cgacgccagg cccgtggaag gttcggtcct gcccggcggc actggcgccc gcttcgactg
961 gggcctgctg gccgggcgac gtttttcgcg gccgcatttc ctggccgggg ggcttgatcc
1021 gtggaacgtc ggtgaggcca tcaagacctc cggaacgccc ctggtggacg tttcttctgg
1081 cgtggagcgt ggtcccggcc taaaggaccc ggctctgatc tcggcgtttc tcgacgccgt
1141 caaacgcgcc aaagactgaa aagcccagct tggaagccct cgtgaacgct cctgcgaaac
1201 ccaatgacta ctcggcctat cccgatgcgg agggccgctt tggcgggttc ggcggccgct
1261 atgtcgccga gaccctgatg ccgctggtcc tggacctggg caaggcctat gccgacgcca
1321 aggccgatcc ggaattccag gcgcagctga aaagctacaa cacccattac gctggccggc
1381 cgagcccgct ctatttcgcc gagcgcctga ccgagcactt cggcggggcc aagatctatt
1441 tcaagcgcga cgagctgaat cacaccggct cgcacaagat caacaacgcc ctgggccaga
1501 tccttctggc catgcgcatg ggcaagaccc ggatcatcgc cgagaccggc gctggccagc
1561 acggcgtggc caccgccacc gtctgcgccc gctttggcct gccctgcgtg gtctatatgg
1621 gcgccaccga cgtcgagcgg cagaagccca atgtcttccg catgaacctg ctgggcgcgg
1681 aagttcgccc cgtcagctcg ggcaccggca ccctgaagga cgccatgaac gaagcgatgc
1741 gcgattgggt gaccaacgtg catgacacct attacctgat cggcacggcc gccggcccgc
1801 acccctatcc ggtgatggtc cgtgacttcc agagcgtgat cggcgccgaa gcgcgtgagc
1861 agatcctcga aatggaaggc cgactgcccg acgcggtggt cgcctgcatc ggcggcggct
1921 ccaacgccat cggcctcttc cacccgttcc tcggcgacga gggtgtgcgc ctgatcggcg
1981 tcgaggcggc cggccacggc gtctccaccg acaagcacgc cgcctcgctg accggcggcc
2041 gtcctggcgt gctgcacggc aaccgcacct acctgctgca ggacgacgac ggccagatca
2101 tcgacgctca ctcgatctcg gctggcctcg actatccggg catcggcccc gagcactcgt
2161 tcctgcacga catcggccgc gccgaatatg tctcgaccac ggacaccgag gccttggagg
2221 ccttcaaact gtgctcgacg ctggaaggca tcatcccggc cctggagccg gcccacgccc
2281 tggcccgggt cggcgagatc gcccaggagc tgggcaaggg caagatcgtg gtgatgaatc
2341 tctgcggtcg cggcgacaag gacatcttca cggtggccga agccctcggg cgcaagatct
2401 aggaccgacc tcgcgtgacc aaagaccgta tcgatcgccg ctttgcggcg ctgaaggccg
2461 aaaaccgcgc cggcttcgtg acctatgtca tggccggcga ccctgacgcc gccacgacgc
2521 tgtccgtgct caagggcctg cccgccgccg gcgcggatct gatcgagctg ggctttccgt
2581 tctctgatcc gatggctgag ggcccgacca tccagcgcgc cgcccagcgg ggcctgaagt
2641 ccggcatgac cctgcagggc acgctggatc tggtggccgc cttccgcgaa ggcgaccagg
2701 acacgccgat catcctgatg ggctatctga acccggttct gaacaagggc tttgagacct
2761 tcgcagcgct ggccgccaag gccggggtcg acggcctgat catcgtcgac tgcccgcccg
2821 aagaggccga cccgctgtcg gacgcgctgg aagccgaggg catcgccctg attcgtctcg
2881 cggccccgac caccgatgac gcccgcctgc cgatggtggt gcgccgcacc tcgggcttcg
2941 tctattacgt ctcggtcgcc ggggtgaccg gagtgaagtc ggccgacgcg gccgatgtgg
3001 cccccgccgt cgagcgcctg cgcaaggcct cgggcctgcc ggtcgccgtg ggctttggta
3061 tccgcacgcc ggaccaggcc gccgccgtcg ccaaggtggc ggacgcggcc gtcgtcggct
3121 cggccctggt cgatgaaatc gagtcggcgg cgcagttgaa cgaaaacgtg accgagaagg
3181 ttcttctaaa agcgtcggag ctggctaagg ctgtgcgctc ggcgcgagtc gggacgaagt
3241 gaggctttaa ggctatggct atggctgaac cccaggaccc gaaaaagggc gacaagaaaa
3301 cagcggagcg ccggggcggc ggttggctgt cgcggatcgc acccggcgtg cgcggcgcgt
3361 tcgccaagcg cgagacgccc gagaacctct gggtcaagtg ccccgatacg ggcgagatga
3421 tcttccgctc cgacctggag gcggccctgt gggtcacgcc ggccggtcgc cacatgcgga
3481 tcgggccgga agcccgcttc aaattcacct tcgacgacgg ccagtacgag gccctgccgg
3541 gccccgccgg tggtcgaaga tc
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