GenBank-Updates@genbank.bio.net (08/24/90)
LOCUS ECOANSORA 2156 bp ds-DNA BCT 24-AUG-1990
DEFINITION E.coli ansA-ORF1 gene pair, complete cds.
ACCESSION M26934
KEYWORDS asparaginase; cytoplasmic protein.
SOURCE E.coli K-12 DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 2156)
AUTHORS Jerlstroem,P.G., Bezjak,D.A., Jennings,M.P. and Beacham,I.R.
TITLE Structure and expression in Escherichia coli K-12 of the
L-asparaginase I-encoding ansA gene and its flanking regions
JOURNAL Gene 78, 37-46 (1989)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable copy of sequence [1] kindly
provided by I.R.Beacham, 07-SEP-1989.
FEATURES from to/span description
pept 161 1129 asparaginase (ansA)
pept 1140 1781 ORF1
signal 1790 1822 transcriptional terminator
BASE COUNT 557 a 562 c 531 g 506 t
ORIGIN
1 aatcctcttc ccgctgtttc gccccatatt tccttatccg catagtatca ggtgcgctcc
61 ccctgcctca cgtatatact tttgctcttt cgatatcatt catatcaata tcatgcaaaa
121 gaaatcaatt tacgttgcct acacgggcgg gaccatcggg atgcagcgtt ccgagcaggg
181 ttatataccg gtgtcaggtc atctacaacg ccaactggcg ctgatgccgg aattccatcg
241 cccggagatg ccagatttca ccattcatga atatacgccg ctgatggatt cttcagatat
301 gacgccggaa gactggcagc atattgctga agatattaaa gcgcactatg acgactatga
361 tggttttgtc attctgcacg gcaccgacac gatggcgtat accgcctctg cgctgtcgtt
421 catgctcgag aatctcggta aaccggtcat tgtgacaggg tcacaaatcc cgctggctga
481 gttacgctct gacggacaaa ttaatctgct gaatgcgttg tacgttgcgg cgaattatcc
541 gatcaacgaa gtaacgctct ttttcaataa ccgattgtat cgcggcaacc gcactaccaa
601 agcccatgcc gatggttttg atgcgtttgc ctctccaaac cttcctccgt tactggaagc
661 aggtatccat attcgtcgtt tgaatacgcc acccgccccg cacggtgaag gggaattaat
721 cgttcatcca atcaccccac aaccaattgg cgtagtgacg atttatccag ggatttctgc
781 tgacgtcgtg cgcaattttc tgcgccaacc ggtgaaagca ttgattctgc gctcctatgg
841 cgtgggtaat gcgccacaaa acaaagcctt cctgcaggaa ttacaagaag ccagcgatcg
901 cggtattgtg gtggtcaacc tgacacaatg tatgtccggt aaagtgaaca tgggtggtta
961 tgccaccggt aacgccctcg cccatgccgg cgtaattggc ggtgcagata tgactgtaga
1021 agccacgcta accaaactgc attacctgct tagccaggaa ctggatactg aaaccattcg
1081 caaggccatg agccaaaacc tgcgcggcga actgacgccg gatgattaag gagactgtaa
1141 tgccccctcg cgccctgtta ctggtcgatt tacaaaatga tttctgtgct ggtggcgcgc
1201 tcgccgtgcc ggaaggtgac agtacggtgg atgtcgctaa ccgcctgatt gactggtgcc
1261 agtcgcgcgg tgaagcggtt atcgccagtc aggactggca cccggcgaat cacggcagtt
1321 ttgccagtca gcacggtgta gagccttata cgccaggcca actcgacggt ttgccacaaa
1381 ccttctggcc agatcactgt gtgcagaaca gtgaaggcgc acaattacat ccgttactgc
1441 accaaaaagc gatcgcagcg gtgttccata aaggcgaaaa tcctttagtt gacagttaca
1501 gtgccttttt tgataacggc cgtcggcaga aaacctctct cgatgactgg ttacgcgatc
1561 atgaaatcga tgaattgatc gttatgggcc tggctactga ctattgcgtg aagtttaccg
1621 tgctggacgc gttacagtta ggttataagg taaacgtgat taccgatggt tgtcgtggcg
1681 tgaatatcca gccccaggac agtgcgcacg cgtttatgga gatgtcagca gctggggcaa
1741 cgctatatac gctggcagac tgggaagaga cacaggggta attttacgct ggcctacaat
1801 tctgtactgg cattgtaggc caaataaaac acgtcagtgg cacatctggc aattgatgcc
1861 atcaacgaaa gatttaattc acctcagaaa tctcttccaa cgacaccttc cgcgtttcga
1921 tgccaaaaat ggagagaacc agcgcacaga gcaataacat aacaccaagt accataaaca
1981 ccgtgatcga cccataatgt gttaataatg ccgcaacgcc atagggcgtg aagactgcga
2041 cgatccgtcc gacggcatta acgaaacccg aaccgcgcag gcgtaaatgc gttggccaaa
2101 gctccgggat ataaaccgcc gacgcgaagc aaacatacat gtataaaaag aagatc
//