GenBank-Updates@genbank.bio.net (08/24/90)
LOCUS R10REP 7053 bp ds-DNA BCT 24-AUG-1990 DEFINITION Plasmid R100 replication-associated protein (repA) genes, complete cds. ACCESSION M26840 KEYWORDS replication-associated protein. SOURCE Plasmid R100 DNA. ORGANISM Plasmid R100 Prokaryota; Bacteria. REFERENCE 1 (bases 1 to 7053) AUTHORS Ohtsubo,H., Ryder,T.B., Maeda,Y., Armstrong,K. and Ohtsubo,E. TITLE DNA replication of the resistance plasmid R100 and its control JOURNAL Adv. Biophys. 21, 115-133 (1986) STANDARD simple staff_entry FEATURES from to/span description pept 90 344 replication-associated protein (repA2) pept 451 636 replication-associated protein (repA3) pept 649 1506 replication-associated protein (repA1) pept 1869 2255 replication-associated protein (repA4) pept 2445 3098 ORF1 pept 3191 3448 ORF2 pept 3381 3782 ORF3 pept 4315 3974 (c) Imm gene product pept 6679 5819 (c) Bla gene product mRNA 397 > 636 mRNA mRNA 545 > 1506 mRNA mRNA 2317 > 3098 mRNA mRNA 3169 > 3448 mRNA mRNA 4338 < 3974 (c) mRNA mRNA 6714 < 5819 (c) mRNA BASE COUNT 1747 a 1701 c 1768 g 1837 t ORIGIN 1 catcttcgtc acaattctca agtcgctgat ttcaaaaaac tgtagtatcc tctgcgaaac 61 gatccctgtt tgagtattga ggaggcgaga tgtcgcacac agaaaatgca gtgacttcct 121 catctggcgc aaaacgagca tacagaaagg ggaatccgct ttctgatgca gagaaacaaa 181 gattatcagt ggcccgtaaa agagcttcgt tcaaggaagt aaaagtattt cttgaaccaa 241 agtataaggc catgctcatg caaatgtgtc atgaagatgg tctgactcag gctgaagttc 301 tgaccgcact gataaaaagt gaagcgcaaa aacgatgcat gtgatgatgg gcttacattc 361 ttgagtgttc agaagattag tgctagatta ctgatcgttt aaggaatttt gtggctggcc 421 acgccgtaag gtggcaagga actggttctg atgtggattt acaggagcca gaaaagcaaa 481 aaccccgata atcttcttca acttttgcga gtacgaaaag attaccgggg cccacttaaa 541 ccgtatagcc aacaattcag ctatgcgggg agtatagtta tatgcccgga aaagttcaag 601 acttctttct gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt 661 caccaaacgt attaccgcca ggtaaagaac ccgaatccgg tgtttacacc ccgtgaaggt 721 gcaggaacgc tgaagttctg cgaaaaactg atggaaaagg cggtgggctt cacttcccgt 781 tttgatttcg ccattcatgt ggcgcatgcc cattcgcgtg gtctgcgtcg acgcatgcca 841 ccagtgctgc gtcgacgggc tattgatgcg ctcctgcagg ggctgtgttt ccactatgac 901 ccgctggcca accgcgtcca gtgctccatc accacgctgg ccattgagtg cggactggcg 961 acggagtctg ctgccggaaa actctccatc acccgtgcca cccgggccct gacgttcctg 1021 tcagagctgg gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt 1081 ccgaccgata tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca 1141 gtggccgccg cgcgccgcag ccgtgtggta tgggaaaaca aacaacgcaa aaagcagggg 1201 ctggataccc tgggcatgga tgaactgata gcgaaagcct ggcgttttgt tcgtgagcgt 1261 tttcgcagtt atcagacaga gcttaagtcc cggggaataa agcgtgcccg tgcgcgtcgt 1321 gatgcggaca gggaacgtca ggatattgtc accctggtga aacggcagct gacgcgcgaa 1381 atcgcggaag ggcgcttcac tgccaatcgt gaggcggtaa aacgcgaagt tgagcgtcgt 1441 gtgaaggagc gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc 1501 ccctgaaagt gacctcctct gaataatccg gcctgcgccg gaggcttccg cacgtctgaa 1561 gcccgacagc gcacaaaaaa tcagcaccac atacaaaaaa caacctcatc atccagcttc 1621 tggtgcatcc ggccccccct gttttcgata caaaacacgc ctcacagacg gggaattttg 1681 cttatccaca ttaaactgca agggacttcc ccataaggtt acaaccgttc atgtcataaa 1741 gcgccatccg ccagcgttac agggtgcaat gtatctttta aacacctgtt tatatctcct 1801 ttaaactact taattacatt catttaaaaa gaaaacctat tcactgcctg tcctgtggac 1861 agacagatat gcacctccca ccgcaagcgg cgggccctta ccggagccgc tttagttaca 1921 acactcagac acaaccacca gaaaaacccc ggtccagcgc agaactgaaa ccacaaagcc 1981 cctccctcat aactgaaaag cggccccgcc ccggcccgaa gggccggaac agagtcgctt 2041 ttaattatga atgttgtaac tacttcatca tcgctgtcag tcttctcgct ggaagttctc 2101 agtacacgct cgtaagcggc cctgacggcc cgctaacgcg gagatacgcc ccgacttcgg 2161 gtaaaccctc gtcgggacca ctccgaccgc gcacagaagc tctctcatgg ctgaaagcgg 2221 gtatggtctg gcagggctgg ggatgggtaa ggtgaaatct atcaatcagt accggcttac 2281 gccgggcttc ggcggtttta ctcctgtatc atatgaaaca acagagtgcc gccttccatg 2341 ccgctgatgc ggcatatcct ggtaacgata tctgaattgt tatacatgtg tatatacgtg 2401 gtaatgacaa aaataggaca agttaaaaat ttacaggcga tgcaatgatt caaacacgta 2461 atcaatatct gcagtttatg ctggttatgc tggctgcatg gggcattagt tggggagcca 2521 gatttgtcat ggagcaggcc gttctgcttt atggatcagg aaaaaactat ttgttcttca 2581 gtcatggtac tgttctgatg tacctgctgt gtgttttcct ggtataccgc cgttggatag 2641 ctccgctacc ggtcgttggt cagctgcgca acgttggcgt accgtggctg gtcggtgcga 2701 tggccgtggt gtatgtcggt gtatttctgc tcggtaaggc gctggctctg cctgctgagc 2761 catttatgac gaaacttttt gccgataagt ccatacctga cgtgatcctg acgttgctag 2821 ccatctttat ccttgccccg ttgaatgagg aaacgctgtt ccgggggatt atgctgaacg 2881 tcttccgttc acggtactgc tggacgatgt ggctgggggc gctgataacg tcgttgttgt 2941 tcgtcgccgc gcacagccag tatcagaacc tgctgacact ggcagaactg ttcctggtgg 3001 ggttgattac atcagtggcc aggatcagaa gtggtggcct gctgctgccg gtattgctgc 3061 atatggaagc aaccacgctg ggtttactgt ttggttgaaa gttatatttt tattaaacat 3121 tgtgcgttaa agcctggtgt gtttttttag tggatgttat atttaaatat aacttttatg 3181 gaggtgaaga atgcatacca cccgactgaa gagggttggc ggctcagtta tgctgaccgt 3241 cccaccggca ctgctgaatg cgctgtctct gggcacagat aatgaagttg gcatggtcat 3301 tgataatggc cggctgattg ttgagccgta cagacgcccg caatattcac tggctgagct 3361 actggcacag tgtgatccga atgctgaaat atcagctgaa gaacgagaat ggctggatgc 3421 accggcgact ggtcaggagg aaatctgaca tggaaagagg ggaaatctgg cttgtctcgc 3481 ttgatcctac cgcaggtcat gagcagcagg gaacgcggcc ggtgctgatt gtcacaccgg 3541 cggcctttaa tcgcgtgacc cgcctgcctg ttgttgtgcc cgtaaccagc ggaggcaatt 3601 ttgcccgcac tgccggcttt gcggtgtcgt tggatggtgt tggcatacgt accacaggtg 3661 ttgtacgttg cgatcaaccc cggacaattg atatgaaagc acggggcgga aaacgactcg 3721 aacgggttcc ggagactatc atgaacgaag ttcttggccg cctgtccact attctgactt 3781 agaacatggg gtttgagggg caactggatg aaaacgtacg atttagggca ctaaaaccgc 3841 tgttgtccca ccattctggt gattcccaaa cgttatttgg ctaaaaagta gttttgatgt 3901 ggtttatttt ccgaattcta tgctcctata ttgataagaa taaacttaat acgataaatg 3961 aggtattagg gatttaatta ttctttattg atataaaaag tcctagcaat ccaaatggga 4021 ttgctaggac caaacaaagt agattatata gcataaatag gtttaatttt gctacggggg 4081 cgttatttag gttttttctt ctttcgaaaa aatgtttctt tatgaagtta aaagctatgt 4141 gttcaatagc atattttgaa tatggacata gaatagtgct tatcactatt gcatatagca 4201 tcttatctgt cacaaggaaa taatactctt cgctgttttt tgttataagg tatatatata 4261 taagtgtgca gtacaggcca aataaaatat tttttatgta gtatcttaag ctcataaatt 4321 aaacctcgtc atatattctt ttcattttat aaggatcgag ttatgaggaa aagatttttt 4381 gtgggaatat tcgcgataaa cctccttgtt ggatgtcagg ctaactatat acgtgatgtt 4441 cagggaggga ctgtggctcc atcatcttct tctgaattgg tggggatcac cgttcagtag 4501 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 4561 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 4621 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 4681 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 4741 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 4801 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 4861 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa 4921 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 4981 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 5041 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 5101 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 5161 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 5221 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 5281 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc 5341 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac 5401 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga 5461 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc 5521 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg 5581 gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc 5641 cagctcgttg agtttctcca gaagcgttaa tggggtctga cgctcagtgg aacgaaaact 5701 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 5761 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 5821 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 5881 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 5941 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 6001 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 6061 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 6121 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 6181 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 6241 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 6301 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 6361 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 6421 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca 6481 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 6541 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 6601 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 6661 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 6721 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 6781 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 6841 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaattt tataaaccgt 6901 ggagcgggca atactgagct gatgagcaat ttccgttgca ccagtgccct tctgatgaag 6961 cgtcagcacg acgttcctgt ccacggtacg cctgcggcca aatttgattc ctttcagctt 7021 tgcttcctgt cggccctcat tcgtgcgctc tag //