GenBank-Updates@genbank.bio.net (08/27/90)
LOCUS CHKACACB 5462 bp ds-DNA VRT 27-AUG-1990
DEFINITION Chicken cardiac alpha-actin gene, clone lambda-AC7, complete cds.
ACCESSION X02212 K02256
KEYWORDS actin; alpha-actin; alpha-cardiac actin.
SOURCE Chicken genomic DNA, clone lambda-AC7 [1],[2].
ORGANISM Gallus gallus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Aves;
Neornithes; Neognathae; Galliformes; Phasianidae.
REFERENCE 1 (bases 841 to 897)
AUTHORS Chang,K.S., Zimmer,W.E.Jr., Bergsma,D.J., Dodgson,J.B. and
Schwartz,R.J.
TITLE Isolation and characterization of six different chicken actin genes
JOURNAL Mol. Cell. Biol. 4, 2498-2508 (1984)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 5462)
AUTHORS Chang,K.S., Rothblum,K.N. and Schwartz,R.J.
TITLE The complete sequence of the chicken alpha-cardiac actin gene: A
highly conserved vertebrate gene
JOURNAL Nucleic Acids Res. 13, 1223-1237 (1985)
STANDARD full staff_review
COMMENT [1] also sequenced part of the 3' terminal fragment.
FEATURES from to/span description
pept 841 969 cardiac alpha-actin, exon 2 (first expressed
exon)
1802 2126 cardiac alpha-actin, exon 3
2752 2913 cardiac alpha-actin, exon 4
3026 3217 cardiac alpha-actin, exon 5
4216 4397 cardiac alpha-actin, exon 6
4757 4900 cardiac alpha-actin, exon 7
pre-msg 299 5075 actin mRNA
IVS 339 820 actin mRNA intron A
IVS 970 1801 actin cds intron B
IVS 2127 2751 actin cds intron C
IVS 2914 3025 actin cds intron D
IVS 3218 4215 actin cds intron E
IVS 4398 4756 actin cds intron F
BASE COUNT 1376 a 1280 c 1179 g 1627 t
ORIGIN 3 bp upstream of SmaI site.
1 cccgggcccg acatctcgcg gacgcaccgc ttgtacattc cggagggcca tggagggcca
61 cagagactcc gagcctcccc tcccaccacg tcggcgggag gctccctatt tggccatgtg
121 gcggcgggac cgctgctcct cacctgcctt agatggccgg acagccgcgc cggccttggc
181 cattcatggc cgcgctgcgc gcatggcgaa gcggcaaata aggagaaggt ggctgcccgg
241 ccgcggacgc cggccccgcc ggggctataa agcggcagct cgcgccccgc cggcacccag
301 gcgctcctcg gtaacctccc gccgtccgtg ctgctccggt gagtggtgct gtaaggcggg
361 acggaaggga tggagagaga gaagaatgga tggggggcag ccgacgggag gcaccgacgg
421 gctcagcttg tgcggtgtta ccggcgtccg cctgctcctg ccgcgagcac gggacgggac
481 accgccctct gcccttctcg ccctcggagc ggctgcggct cgacggggcg cggtgcgccg
541 gaggcggcgt gcgctggcag agccggactc tgcgatacca tttcgctcat ccattttgat
601 ctgtgcccat agggtaccgc tagtgatgcc caaagttagc gttctgggag ccttctgaat
661 cgtgaacggc agcggcgacg ggtccctcag agccgtgccc gagcatggcc gagccccgcg
721 gacccgccga gccacccgct gccaccgggg ctgcccctgt ccccgggggt gccccgaggt
781 gcccgcagag gtctgtatgc gccgccgcgc tgtcttgcag gtccgtgcct atcagccaag
841 atgtgtgacg acgaggagac caccgcgctg gtgtgcgaca acggctcggg gctggtcaag
901 gcgggctttg ctggggacga tgcccccagg gccgtgttcc catccatcgt cggccgcccc
961 aggcaccagg taaattcctg cccgatgaca gaaccgggct ccattctctc atcaggagtc
1021 agccccactc cttaattctg tccttctcct gccaccccag ccttctcttc ccttgtgtcc
1081 tactgacaca ttttctgttg tctgtgcttc ttccctacat ttccctcttt ggctcaccat
1141 catcttatct ctgtcactgt caccccctgt accagttctt tcccaattca ccagtatccc
1201 ttctctctcc cccaatgagt ccaaacccat tttcctccag aactgcttcc cacaccatcc
1261 cctaagtgag ataagctgct ggtgttggtt caaagacatc tccatgggct gaatttgtcc
1321 cacccatcca tgtcccacat atcaagccct tgcatttcct ctgctgcttt tgtctgtctc
1381 tgccttctta ccaccttcta agccttcttt tctggggtcc ccatttcatt ctccttgtat
1441 tttctctcag tttgttcaaa acacagaaaa cgtagggctt tttcttttat aatttctgtg
1501 gcaaaaactt atcatgcttt tcactgtcaa ttgaacttct ggtccagaaa gaggtaattg
1561 aaaataaagg atatattaaa aaaaaatagt gggaaaagtc ctttgatctg attaaaggaa
1621 aaataacttt gagaacttca agttctgttg agttaatatc ttaataaaca actttaacaa
1681 ccttttagtg gccactactg tttgtacctc tctggaaaat agcatagttg attgaggtat
1741 aaatctagct ctgtggtgat gtctttctca aatcatgcgt tcctgtattt tgttctttta
1801 gggtgttatg gtcggtatgg gtcaaaaaga ttcctatgtg ggtgatgaag cacagagcaa
1861 aagaggtatc cttactttga aatatcctat tgaacatggc atcatcacca actgggacga
1921 tatggaaaag atctggcacc acactttcta caatgaactc cgtgtggccc ctgaggagca
1981 ccccaccttg ctcacagaag cacccttgaa ccctaaagct aatcgtgaaa agatgaccca
2041 gataatgttt gagaccttca atgtacccgc tatgtatgtg gcaatccagg ctgtcctgtc
2101 cctgtatgcc tctggacgta ccacaggtaa aatagacttc tttcctttca ggacccacag
2161 accctccttc cttcccactc caaacacaca gacacacaca cacagaacaa cgtcgatgtt
2221 caaatttaca gaaaactatg ctgttggctt ttttattatt attatattta ttaaaggtaa
2281 ctaactagca gaaaagctag tctgtgaaag gaactatata aatttgtctg cattggttta
2341 aaagaaaggc attaaagaac ctgattttcc tgctgcttgt catgatgcaa acaaggagac
2401 atcactgcag ttagcacaag tattacacca gtttgagttt aaactgagcc taagagttgc
2461 cctttactgt atcaaggcgg ttgctaccat caatacactt gctgctcagt gtagcattgg
2521 taggctattc atatggattc attagaagtc tgtatgggac aattttttat ggggattgtt
2581 tgaccaaatg ggaatattgg aaggagttgg caagacctgc atggcagtag ctggctaaaa
2641 tagaatgacg ctgcttatac tacctgaaaa tgcatgtctg atatctgtgg tactgctgtg
2701 ttgtaaggtt tctcaggtta ttccaactgg tttctgacat ttcttttcca ggtattgttc
2761 ttgactctgg tgatggagtc acccacaacg tgcccattta tgaaggttat gctttgcctc
2821 atgctatcat gcgtctggat ctggctggca gggatctgac cgactacctc atgaagatcc
2881 tcacagagcg tggctattcc tttgtgacca caggtcagtc cttcagcttg accctcttct
2941 gaatatcctc atgtctccct tggcccacct tctttccctg atattttgct tctccaaact
3001 tgatttgatt tctgtgtacc tgcagctgaa cgtgagattg ttcgtgatat caaagagaaa
3061 ttgtgctatg ttgcgctgga ctttgagaat gaaatggcca cagctgcttc gtcatcctcc
3121 ctggagaaga gctatgaatt gcctgatggt caagtgatca caattggtaa tgaacgtttc
3181 cgctgcccgg aaaccttatt ccagccatcc tttattggta agtgcttcaa ggaaattcct
3241 ttttcagtag gaggtgcaaa tgaatcagaa gttctagaag gtgtcccact ccccctcctg
3301 tttatctaca ttttgcacaa aatcaactat aaataaggag tttgattctc tgctccctgt
3361 ttgctgtgct taatgtgatt actagctaat aattagctga tctatggact cctgttgcaa
3421 ctctagggcc agcttttaag ttcacaattt ttatgatgga atgtacactg acaatgtgtc
3481 aatgcacaat gggcaagagc agctttcagt gctgtatttg taccacctgc acttcaaaca
3541 ctatctgatt ccatgaagaa gtaaaatgtg tgggagataa aaagatgtaa tgaagaagtg
3601 tatatttaca tgcactgaaa ttaaatcttg ccgtggacaa gatggctggt tttgctgtgt
3661 gtagatgcag ctgacagaac agcaaatgct tataatctaa gcccaagcaa caaatgcagt
3721 tgttggtcag ctgatgtgtt actgtggatt tagtgtcagt tctttcagga agcacctgtt
3781 aaaattataa attggaatgc aacctacgaa cacttgcttc tggtttcttt ttacttatca
3841 ccaaatgcta acatcctcat ctatgtcttg gtcttgtctt catgtttata aatggcatag
3901 actcacaata aatgagttta tcttatgtgt ctaactcttc tcttactgta ggtcttgtgt
3961 cttttctttg cacatgcatt atgattctta gcttttctaa taaataatat tagttttcta
4021 agcttgtcct atagttctgg gttgtttttt gggttttttt tttttttttt gtatttttct
4081 gtacaactta gtaatcaata agcaatttta gaacaaaaca ataaaagttc atatttatag
4141 tgaggagaaa tgctgagtgt ccttttcaca aagacctcat gtataattta ctgcatcagc
4201 ttcttttttc tcaaggtatg gaatctgctg gtattcatga aactacctac aacagcatca
4261 tgaagtgtga tattgatatt cgtaaggatc tgtatgctaa caacgtcctc tcaggtggta
4321 caacaatgta ccctggtatt gctgatcgca tgcagaaaga aatcactgca cttgctccta
4381 gcacaatgaa gatcaaggtg aggactttga ttctactaaa aaatttgaat aaggtttagg
4441 aaaatgtcat taaattagga tacaggaaca actcataaat gagaccatca gctgatgggc
4501 ggtccggcca agaagaaata ttttcatttc acttcattaa tattataaga tattaattga
4561 agcaatgtgc aaatcaggaa aattgatctg tagatttagt taaagaatat tacattacca
4621 gctgctgaag accagtacat tactatacat tattacttta agaaacacct agcagtttgg
4681 tgtgttgatg atgtgggtta gttgtgcagg aattgaactg ttaacgtagg attttggctg
4741 tttctcttca tcctagatca ttgctccacc tgaacgcaag tactctgtct ggattggtgg
4801 ctccattcta gcatctttgt ccaccttcca gcaaatgtgg atcagcaaac aggagtatga
4861 tgaggctggt ccatccattg tccaccgcaa gtgcttctaa gacttttccc tacatttact
4921 tcccactcag gatgatgaca gtatgcttct tggagtcttc tggcagccct tcctgaactc
4981 ctccgtactt gtacagtttg tttacacact tgtgcaattt atttgtgctt ctaatattta
5041 tgctttataa ataaagatct ggacttgcaa cctacaaatg tttctgattc ttttattcta
5101 agcgttattt ggttaaaaat gagtctgtga atacctggat ttggaaactc ccatcagatt
5161 tgtcatacgt gtctttctaa gtgatgtgga catgttaggt catatttcat atttatagtc
5221 ttaatttgta aacttgaaac aaaagaacta tcatttacaa gaaagtaacc ttgagggtaa
5281 gacccaagga gtggaacaag aaggaaacct ctcacattca ctgaatgggc ttagaattgc
5341 tttttttcca ttgcgttaat tataacctca gtaatttaat atcaacttac tgaaagccaa
5401 agcctctcaa gaaggattat gctaatacac ctagaaagca atttataact aattaactca
5461 gt
//