GenBank-Updates@genbank.bio.net (08/28/90)
LOCUS BSUTRNASA 2325 bp ds-DNA BCT 28-AUG-1990
DEFINITION B.subtilis threonyl-tRNA synthetase (thrS2) gene, complete cds.
ACCESSION M36593
KEYWORDS threonyl-tRNA synthetase.
SOURCE B.subtilis (strain 168) DNA.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes;
Endospore-forming rods and cocci; Bacillaceae.
REFERENCE 1 (bases 1 to 2325)
AUTHORS Putzer,H., Brakhage,A. and Grunberg-Manago,M.
TITLE Independent genes for two threonyl-tRNA synthetases in Bacillus
subtilis
JOURNAL J. Bacteriol. (1990) In press
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by H.Putzer, 07-MAY-1990.
Threonyl-tRNA synthetase (thrS2) is not expressed during vegetative
growth.
FEATURES from to/span description
pept 280 2196 threonyl-tRNA synthetase (thrS2) (EC 6.1.1.3)
binding 265 270 ribosome binding site
BASE COUNT 657 a 478 c 629 g 561 t
ORIGIN Map position of 344 degrees.
1 ttgaatacat gcgatgaaaa ggacatgaat gatagttacg gttttcagag agggaaggaa
61 aggtgagagc ttcccagctt aggaatatca tttaccgcct tggagcagca ttggggaact
121 gaagtaacca atgccgtcta tgctgcgtta cagcaccgag ccgacaacat agtatttgtc
181 gggaacttgg gtggaaccac gggttaatca cacactcgtc cctatctgcg ggacgggtgt
241 gtttttttat atacaacaat gaataaggag tgttctaaaa tgagcaaaca tgtacacatt
301 cagcttccgg acggacagat ccaggaatat ccgaaaggca tcacaatcaa agaagcggct
361 ggttcgatca gttccagtct gcaaaagaag gcggccgcag gtcaggtcaa cggcaagctt
421 gttgatctca gtttcaagct ggaggaggat gctgagcttt cgattgtcac actcgattca
481 caagaaggct tgcaggtcgt tcgccacaca acagcgcacg tgctggccca agcagtaaaa
541 cggctgtacg gcgaagtgtc attgggggta gggcctgtga ttttagacgg cttctactat
601 gacatgaagc tggggaaaag cttggcatca ggagacttag aggccatcga aaaagaaatg
661 aaaaacataa taaatgagaa ccttgaaatc aaacgtatag aggtttctta cgaagaagct
721 gaagagctgt ttgcacaaaa agacgagcgg ctcaagcttg agattttaaa ggatattccg
781 cgcggggagg atatcacact gtaccagcaa ggggaattcg ttgacctgtg ccgagggccg
841 catcttcctt ctacagggat gatcaaggcg ttcaaactga caagagtatc aggcgcttat
901 tggcgcggag acagcaaaaa tgaagtcctg cagcgggtgt acggggttgc gtttcaaaag
961 aaaaaagatt tggatgcgca cctgcatatg ctagaagaag cggcaaaacg agaccatcgc
1021 aagctcggca aacagttagg gctgtttatg ttttctgaag aagcgccggg gatgccgttt
1081 tatttgccga aagggcagat tgtccgcaat gagctggagc gcttctcgcg tgagctgcaa
1141 acaaatgccg gctacgatga ggtgcgtacg ccgtttatga tgaatcagcg gttatgggag
1201 cagtcaggcc attgggacca ttatcgcgac aatatgtact tttcagaagt ggatgataca
1261 agattcgcaa tgaagccgat gaattgcccc ggccacatgc tcatctttaa aaacagcctg
1321 tattcttatc gggatctgcc gattcgcatg gcggaattcg gacaggtgca ccgccatgaa
1381 tacagcggcg ctttaaacgg aatgcttcgc gttcggacgt tctgtcagga tgacgcccat
1441 attttcgttc gggaggatca gattgagagt gagatcaaag aagcgattcg gctgattgat
1501 gaggtatatc ggacatttgg tttcgagtat tctgttgagc tttcgactcg cccggaggat
1561 tcattaggcg atgacagcct ttgggaagcg tcggaacgtg ccttagcacg cgtccttgag
1621 gagcttggcc tctcttatga gatcaatgaa ggcgatgggg cgttttacgg gccgaaaatt
1681 gatttccata taaaagatgc cctgaaacgg agccaccaat gcgcgacaat tcagcttgat
1741 tttcaaatgc cggaaaaatt tgatttaaca tatatcaacg aactcaatga gaaggttcgt
1801 cccgttgtca tccacagagc ggttttcggc tcaatcgacc gtttcttcgg cattttgatc
1861 gagcattacg gtggggcttt tccagtctgg ctcgcaccga tccaggttca aatcataccg
1921 gtttcgcacg ttcacttaga ctattgcaga aaagtgcagg cggagctgaa gcaggcgggg
1981 ataagagccg gcattgatga gcgcaatgag aagctcggct ataaaatcag agaatcgcag
2041 gtgcaaaaaa ttccgtacgt gctggtgctt ggagatcatg aggagcagga gaatgcggta
2101 aacgtccgcc gattcggaca tcagcagaat gaacacgttc ctttccaaac ttttaaagat
2161 aaacttgtga aacaggtgga aaatcgaggc atgtaggata aaagctgtga attcacattt
2221 tgcttttttg actttcccta ttcggaatca gtaaaataat aagtaaatca tatcgttcgt
2281 atgttatgaa tgcatctcga aatggggaaa ttgacatgga ccatg
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