GenBank-Updates@genbank.bio.net (08/28/90)
LOCUS BSUTRNASB 2500 bp ds-DNA BCT 28-AUG-1990
DEFINITION B.subtilis threonyl-tRNA synthetase (thrSv) gene, complete cds.
ACCESSION M36594
KEYWORDS threonyl-tRNA synthetase.
SOURCE B.subtilis (strain 168) vegetative form DNA.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes;
Endospore-forming rods and cocci; Bacillaceae.
REFERENCE 1 (bases 1 to 2373)
AUTHORS Putzer,H., Brakhage,A. and Grunberg-Manago,M.
TITLE Independent genes for two threonyl-tRNA synthetases in Bacillus
subtilis
JOURNAL J. Bacteriol. (1990) In press
STANDARD full staff_entry
REFERENCE 2 (bases 1 to 2500)
AUTHORS Putzer,H., Brakhage,A. and Grunberg-Manago,M.
JOURNAL Unpublished (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [2] kindly submitted
by H.Putzer, 07-MAY-1990.
Threonyl-tRNA synthetase found in vegetative from.
FEATURES from to/span description
pept 365 2296 (thrSv) (EC 6.1.1.3)
signal 27 32 -35 region
signal 50 55 -10 region
binding 350 356 ribosome binding site
signal 2300 2337 transcription terminator
BASE COUNT 783 a 521 c 613 g 583 t
ORIGIN Map position of 250 degrees.
1 tttaaaaaaa aggaaacgcg atcgtgttga ttttttggat tgaacaattt ataatacata
61 ggagattaag aaagacacac gtattgataa ggacacgaag gaatgatacc ggctcaaaga
121 gagggaagcc ctggtgcaag cttcccagct aaggaccatt cgctttaccg cctttgaact
181 tcagctgtga acctcttgaa aaggaacagt aatggctgac gggaactccc gttacagagc
241 ttagagccgc aggtgccgtg tattggcttt gcggaaaaaa gggtggaacc acgattccgt
301 ttattcaacc tcgtcccttt catagggggc ggggttttta tatgcaaaaa aaggagtgac
361 aaagatgtca gatatggtaa aaatcacatt tcctgatgga gcagtcaagg agtttgcgaa
421 aggaacaaca acagaagata tcgcggcatc catcagtccg ggattaaaga aaaagtcatt
481 agccggaaaa ctgaacggaa aagaaatcga tttgagaacg ccgatcaatg aagacggtac
541 agtggaaatc attacagaag gctcagaaga aggtcttcaa attatgcgcc acagtgcggc
601 tcacctgctg gctcaagcga ttaaacgcat ctacaaggat gttaaattcg gcgtcggtcc
661 ggttatcgaa aacggttttt actacgatgt agaaatggac gaagcgatta caccggagga
721 tctgccgaaa atcgagaaag aaatgaaaaa aatcgttaat gcgaaccttc cgatcgttcg
781 aaaagaagtc agccgtgaag aagcgaaagc ccgttttgcg gaaatcggcg acgacctgaa
841 gcttgaacta ttggatgcga ttcctgaagg agaaaccgtt tcgatctatg agcaaggcga
901 attctttgac ctgtgccgcg gcgtccatgt tccttcaacc ggaaaaatca aagaatttaa
961 gctgttaagc cttgcaggcg catactggcg cggtgacagc aaaaaccaaa tgcttcagcg
1021 cgtatatggt acagctttct tcaaaaaagc tgatcttgaa gagcatcttc gtatgctaga
1081 agaagcgaaa gaacgcgacc acagaaagct tggcaaagaa ttaaagctgt tcgcgaactc
1141 tcaaaaagtc ggacaaggcc tgccgctttg gctgccaaaa ggcgcaacaa tccgccgcgt
1201 catcgagcgc tacattgtcg ataaagaaat cagcctcggc tatgagcacg tatacacacc
1261 tgtgctaggc agcaaagagc tgtatgaaac atcaggacac tgggatcatt atcaggaagg
1321 catgttccct ccgatggaaa tggacaatga aacacttgta ttgcgtccga tgaactgtcc
1381 gcaccatatg atgatttaca aacaagacat tcacagttac cgcgagcttc cgattcgtat
1441 tgcagagctt ggaacgatgc accgctatga aatgtcaggt gcgctgtcag gacttcaacg
1501 ggtacgcgga atgacgttaa atgatgcaca catctttgtg cgcccggatc aaattaagga
1561 tgagtttatc cgtacagtcc gtttaatcca ggatgtttat gaagacttcg gtttaagtga
1621 ttacacattc cgtctgtctt accgcgatcc ggaagataca gagaaatatt ttgatgacga
1681 tgaaatgtgg aacaaagcgc aatccatgct gaaagaggca atggatgaaa tcggccacga
1741 ctattacgaa gcagaaggtg aagcggcatt ctacggacct aaacttgatg ttcaggtgaa
1801 aactgcgatc ggaaaagaag aaacactgtc tacggttcag cttgatttct tattgccgga
1861 acgtttcgac ctgacataca tcggtgagga cggcaagcag caccgtccgg ttgtcattca
1921 cagaggtgtc gtttcaacaa tggaacgctt tgttgctttc ttaatcgaag aacacaaagg
1981 cgcgctgccg acatggcttg caccggttca gttccaagtc atcccggttt ctccggctgt
2041 gcatttagac tacgcgaaaa aagtgcagga acgcctgcaa tgtgaaggcc tgcgtgttga
2101 agtcgacagc cgcgatgaaa aaatcggcta caaaatccgt gaagcgcaaa tgcaaaaaat
2161 cccgtacatg ctggtggtcg gtgaccaaga agcagaaaac ggagcggtaa acgtgcgtaa
2221 atacggagaa cagaactctg aaaccatttc acttgatgag tttgtgaaaa aggcagtagc
2281 tgaagcgaaa aaataaaata aaaaagcatg atctcattga agagatcatg ctttttttat
2341 ttctctaata taggaaacaa atcatgaagg ctgctgatga tgtaatcagg ctttacgtca
2401 gtttcgtttt tcttatcagt ccggttgatc caaacggttt tgattccggc tcttgaagcg
2461 cctaaaatat cagtattcaa attatcgccg accatgatgg
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