GenBank-Updates@genbank.bio.net (08/28/90)
LOCUS TIPNPSS 6429 bp ds-DNA SYN 28-AUG-1990
DEFINITION A.tumefaciens T-DNA vector containing octopine T-DNA borders and
markers: neomycin-phosphotransferase - octopine synthase (3' end)
and Sp/Sm adenyltransferase. complete cds.
ACCESSION M35007
KEYWORDS neomycin phosphotransferase;
streptomycin/spectinomycin adenyltransferase.
SOURCE N.tabacum T-DNA inserts in A.tumefaciens DNA.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (bases 1 to 6429)
AUTHORS Gheysen,G.D.R., Herman,L., Breyne,P., Gielen,J., Van Montagu,M. and
Depicker,A.
TITLE Cloning and sequence analysis of truncated T-DNA inserts from
Nicotiana tabacum
JOURNAL Gene (1990) In press
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by G.D.R.Gheysen, 01-JUN-1990.
FEATURES from to/span description
pept 2782 1985 (c) neomycin phosphotransferase (gtg start codon)
pept 5013 4042 (c) streptomycin/spectinomycin adenyltransferase
(gtg start codon)
mRNA / 1811 1105 (c) octopine synthase (3' end)
recomb 24 25 T-DNA end/plant DNA start
recomb 40 41 plant DNA end/T-DNA start
recomb 1094 1095 plant DNA end/T-DNA start
recomb 2786 2787 T-DNA end/plant DNA start
recomb 3044 3045 T-DNA end/plant DNA start
recomb 3354 3355 plant DNA end/T-DNA start
recomb 5589 5590 T-DNA end/plant DNA start
recomb 6393 6394 T-DNA end/plant DNA start
signal 1650 1645 (c) poly-A signal
signal 1686 1681 (c) poly-A signal
site 1634 1633 (c) major poly-A site
site 1 24 left T-DNA border
site 372 395 24 bp border-like sequence
site 1569 1592 24 bp border-like sequence
site 1669 1692 24 bp border-like sequence
site 1779 1756 (c) 24 bp border-like sequence
site 2128 2105 (c) 24 bp border-like sequence
site 2449 2472 24 bp border-like sequence
site 2485 2462 (c) 24 bp border-like sequence
site 3660 3683 24 bp border-like sequence
site 3875 3898 24 bp border-like sequence
site 4359 4336 (c) 24 bp border-like sequence
site 5872 5895 24 bp border-like sequence
site 6406 6429 right T-DNA border
BASE COUNT 1511 a 1754 c 1711 g 1453 t
ORIGIN
1 cggcaggata tattcaattg taaatggctt catgtccggg aaatctacat ggatcagcaa
61 tgagtatgat ggtcaatatg gagaaaaaga aagagtaatt accaattttt tttcaattca
121 aaaatgtaga tgtccgcagc gttattataa aatgaaagta cattttgata aaacgacaaa
181 ttacgatccg tcgtatttat aggcgaaagc aataaacaaa ttattctaat tcggaaatct
241 ttatttcgac gtgtctacat tcacgtccaa atgggggctt agatgagaaa cttcacgatc
301 gatgccttga tttcgccatt cccagatacc catttcatct tcagattggt ctgagattat
361 gcgaaaatat acactcatat acataaatac tgacagtttg agctaccaat tcagtgtagc
421 ccattacctc acataattca ctcaaatgct aggcagtctg tcaactcggc gtcaatttgt
481 cggccactat acgatagttg cgcaaatttt caaagtcctg gcctaacatc acacctctgt
541 cggcggcggg tcccatttgt gataaatcca ccatcacaat agatagtcta atggacgaaa
601 aaggcgaata tttcgatgct gagattcgac gcaattaatt cgagaaaaat cccgtgattg
661 atgctgttga gttaccaata atatgggcag cgaaggccat ttaattataa gatcctgcaa
721 gcctcgtcgt cctggccgga ccacgctatc tgtgcaaggt ccccggcccc ggacgcgcgc
781 tccatgagca gagcgcccgc cgccgaggcg aagagtcggg cggcgccctg cccgtcccac
841 caggtcaaca ggcggtaacc ggcctcttca tcgggaatgc gcgcgacctt cagcatcgcc
901 ggcatgtccc cctggcggac gggaagtatc cagctcgacc aaagcggcca tcgtgcctcc
961 ccactcctgc agttcggggg catggatgcg cggatagccg ctgctggttt cctggatgcc
1021 gacggatttg cactgccggt agaactccgc gaggtcgtcc agcctcaggc agcagctgaa
1081 ccaactcgcg aggggatcga gcccctgctg agcctcgaca tgttgtcgca aaattcgccc
1141 tggacccgcc caacgatttg tcgtcactgt caaggtttga cctgcacttc atttggggcc
1201 cacatacacc aaaaaaatgc tgcataattc tcggggcagc aagtcggtta cccggccgcc
1261 gtgctggacc gggttgaatg gtgcccgtaa ctttcggtag agcggacggc caatactcaa
1321 cttcaaggaa tctcacccat gcgcgccggc ggggaaccgg agttcccttc agtgaacgtt
1381 attagttcgc cgctcggtgt gtcgtagata ctagcccctg gggccttttg aaatttgaat
1441 aagatttatg taatcagtct tttaggtttg accggttctg ccgctttttt taaaattgga
1501 tttgtaataa taaaacgcaa ttgtttgtta ttgtggcgct ctatcataga tgtcgctata
1561 aacctattca gcacaatata ttgttttcat tttaatattg tacatataag tagtagggta
1621 caatcagtaa attgaacgga gaatattatt cataaaaata cgatagtaac gggtgatata
1681 ttcattagaa tgaaccgaaa ccggcggtaa ggatctgagc tacacatgct caggtttttt
1741 acaacgtgca caacagaatt gaaagcaaat atcatgcgat cataggcgtc tcgcatatct
1801 cattaaagca gggggtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc
1861 cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa
1921 tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc
1981 ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
2041 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
2101 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
2161 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
2221 tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg
2281 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
2341 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
2401 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
2461 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
2521 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
2581 gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa
2641 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
2701 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
2761 atccatcttg ttcaatccac atgatcagat ctctaggcgc gtgggtgcgg acgtagtcag
2821 cgccattgcc gatcgcgtga agttccgccg caaggccgct ggacccagat cctttacagg
2881 aaggccaacg gtggcgccca agaaggattt ccgcgacacc gagaccaata gcggaagccc
2941 caacgccgac ttcagctttt gaaggttcga cagcacgtgc agcgatgttt ccggtgcggg
3001 gctcaagaaa aatcccatcc ccggatcgag gatgagccgg tcggcagcga ccccgctccg
3061 tcgcaaggcg gaaacccgcg cctcgaagaa ccgcacaatc tcgtcgagcg cgtcttcggg
3121 tcgaaggtga ccggtgcggg tggcgatgcc atcccctgcg ctgagtgcat aaccaccagc
3181 ctgcagtccg cctcagcaat atcgggatag agcgcagggt caggaaatcc ttggatatcg
3241 ttcaggtagc ccacgccgcg cttgagcgct agcgcgggtt tccggttgga agctgtcgat
3301 tgaaacacgg tgcatctgat cggacagggc gtctaagagc ggcgcaatac gtctgatctc
3361 atcggccggc gatacaggcc tcgcgtccgg atggctggcg gccggtccga catccacgac
3421 gtctgatccg actcgcagca tttcgaccgc cgcggtgaca gcgttggtgg ggtctagcag
3481 tacgtcaatc gaagaaggag tcctcggtga gattcagaat gccgaacacc gtcaccatgg
3541 cgtcggcctc cgcagcgact tccacgatgg ggatcgggcg agcaaaaagg cagcaattat
3601 gagccccata cctacaaagc cccacgcatc aagcttttga ccctgaagca actaggcaat
3661 ggctgtaatt atgacgacgc cgagtcccga accagactgc ataagcaaca accgacagaa
3721 tggatttcga aaccagagaa agaaaataaa tgcgatgcca taaccgatta tgaacaacgg
3781 cggaaggggc aagcttagta aatgcctcgc tagattttaa tgcggatgtt gcgattactt
3841 cgccaactat tgcgataaca agaaaaagcc agcctttcat gatatatctc ccaatttgtg
3901 tagggcttat tatgcacgct taaaaataat aaaagcagac ttgacctgat agtttggctg
3961 tgagcaatta tgtgcttagt gcatctaatc gcttgagtta acgccggcga agcggcgtcg
4021 gcttgaacga attgttagac attatttgcc gactaccttg gtgatctcgc ctttcacgta
4081 gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt cttgtccaag
4141 ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc gctccattgc
4201 ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt accaaatgcg
4261 ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg agttccatag
4321 cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat caaagagttc
4381 ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca gcaagatagc
4441 cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat tgcgctgcca
4501 ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac
4561 aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc
4621 caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac
4681 cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg
4741 tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg
4801 agtcgatact tcggcgatca ccgcttccct catgatgttt aactttgttt tagggcgact
4861 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
4921 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacatg tcataacaag
4981 aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc
5041 agttgcgtga ggccatacgc tacttgcatt acagcttacg aaccgaacag gcttatgtcc
5101 actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca
5161 gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg gtctccacgc
5221 atcgtcaggc attggcggcc ttgctgttct tctacggcaa gtgctgtgca cggatctgcc
5281 ctggcttcag gagatcggaa gacctcggcc gtccgggcgc ttgccggtgg tgctgacccc
5341 ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagct
5401 tctgtatgga acgggcatgc ggatcagtga gggtttgcaa ctgcgggtca aggactggat
5461 ttcgatcacg gcacgatcat cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg
5521 ttacccgaga gcttggcacc cagcctgcgc gagcagctgt ctcgtgcacg ggcatggtgg
5581 ctgaaggact aggccgaggg ccgcagcggc gttgcgcttc ccgacgccct tgagcggaag
5641 tatccgcgcg ccgggcattc ctggccgtgg ttctgggttt ttgcgcagca cacgcattcg
5701 accgatccac ggagcggtgt cgtgcgtcgc catcacatgt atgaccagac ctttcagcgc
5761 gccttcaaac gtgccgtaga acaagcaggc atcacgaagc ccgccacacc gcacaccctc
5821 cgccactcgt tcgcgacggc cttgctccgc agcggttacg acattcgaac cgtgcaggat
5881 ctgctcggcc attccgacgt ctctacgacg atgatttaca cgcatgtgct gaaagttggc
5941 ggtgccggag tgcgctcacc gcttgatgcc tgccgcccct cactgtgaga ggtagggcag
6001 cgcaagtcaa tcctagcgga ttcactaccc ctgcgcgaag gccatcggtg ccgcatcgaa
6061 cggccggttg cggaaagtcc tccctgcgtc cgctgatggc cggcagcagc ccgtcgttga
6121 aggatccctg aaagcgacgt tggatgttaa catctacaaa ttgccttttc ttacgaccat
6181 gtacgtaagc gcttacgttt ttggtggacc cttgaggaaa ctggtagctg ttgtgggcct
6241 gtggtctcaa gatggatcat taatttccac cttcacctac gatggggggc atcgcaccgg
6301 tgagtaatat tgtacggcta agagcgaatt tggcctgtag acctcaattg cgagctttct
6361 aatttcaaac tattcgggcc taacttttgg tgtgatgatg ctgactggca ggatatatac
6421 cgttgtaat
//