GenBank-Updates@genbank.bio.net (08/28/90)
LOCUS TIPNPSS 6429 bp ds-DNA SYN 28-AUG-1990 DEFINITION A.tumefaciens T-DNA vector containing octopine T-DNA borders and markers: neomycin-phosphotransferase - octopine synthase (3' end) and Sp/Sm adenyltransferase. complete cds. ACCESSION M35007 KEYWORDS neomycin phosphotransferase; streptomycin/spectinomycin adenyltransferase. SOURCE N.tabacum T-DNA inserts in A.tumefaciens DNA. ORGANISM Cloning vector Artificial sequences; Cloning vehicles. REFERENCE 1 (bases 1 to 6429) AUTHORS Gheysen,G.D.R., Herman,L., Breyne,P., Gielen,J., Van Montagu,M. and Depicker,A. TITLE Cloning and sequence analysis of truncated T-DNA inserts from Nicotiana tabacum JOURNAL Gene (1990) In press STANDARD full staff_review COMMENT Draft entry and computer-readable sequence for [1] kindly submitted by G.D.R.Gheysen, 01-JUN-1990. FEATURES from to/span description pept 2782 1985 (c) neomycin phosphotransferase (gtg start codon) pept 5013 4042 (c) streptomycin/spectinomycin adenyltransferase (gtg start codon) mRNA / 1811 1105 (c) octopine synthase (3' end) recomb 24 25 T-DNA end/plant DNA start recomb 40 41 plant DNA end/T-DNA start recomb 1094 1095 plant DNA end/T-DNA start recomb 2786 2787 T-DNA end/plant DNA start recomb 3044 3045 T-DNA end/plant DNA start recomb 3354 3355 plant DNA end/T-DNA start recomb 5589 5590 T-DNA end/plant DNA start recomb 6393 6394 T-DNA end/plant DNA start signal 1650 1645 (c) poly-A signal signal 1686 1681 (c) poly-A signal site 1634 1633 (c) major poly-A site site 1 24 left T-DNA border site 372 395 24 bp border-like sequence site 1569 1592 24 bp border-like sequence site 1669 1692 24 bp border-like sequence site 1779 1756 (c) 24 bp border-like sequence site 2128 2105 (c) 24 bp border-like sequence site 2449 2472 24 bp border-like sequence site 2485 2462 (c) 24 bp border-like sequence site 3660 3683 24 bp border-like sequence site 3875 3898 24 bp border-like sequence site 4359 4336 (c) 24 bp border-like sequence site 5872 5895 24 bp border-like sequence site 6406 6429 right T-DNA border BASE COUNT 1511 a 1754 c 1711 g 1453 t ORIGIN 1 cggcaggata tattcaattg taaatggctt catgtccggg aaatctacat ggatcagcaa 61 tgagtatgat ggtcaatatg gagaaaaaga aagagtaatt accaattttt tttcaattca 121 aaaatgtaga tgtccgcagc gttattataa aatgaaagta cattttgata aaacgacaaa 181 ttacgatccg tcgtatttat aggcgaaagc aataaacaaa ttattctaat tcggaaatct 241 ttatttcgac gtgtctacat tcacgtccaa atgggggctt agatgagaaa cttcacgatc 301 gatgccttga tttcgccatt cccagatacc catttcatct tcagattggt ctgagattat 361 gcgaaaatat acactcatat acataaatac tgacagtttg agctaccaat tcagtgtagc 421 ccattacctc acataattca ctcaaatgct aggcagtctg tcaactcggc gtcaatttgt 481 cggccactat acgatagttg cgcaaatttt caaagtcctg gcctaacatc acacctctgt 541 cggcggcggg tcccatttgt gataaatcca ccatcacaat agatagtcta atggacgaaa 601 aaggcgaata tttcgatgct gagattcgac gcaattaatt cgagaaaaat cccgtgattg 661 atgctgttga gttaccaata atatgggcag cgaaggccat ttaattataa gatcctgcaa 721 gcctcgtcgt cctggccgga ccacgctatc tgtgcaaggt ccccggcccc ggacgcgcgc 781 tccatgagca gagcgcccgc cgccgaggcg aagagtcggg cggcgccctg cccgtcccac 841 caggtcaaca ggcggtaacc ggcctcttca tcgggaatgc gcgcgacctt cagcatcgcc 901 ggcatgtccc cctggcggac gggaagtatc cagctcgacc aaagcggcca tcgtgcctcc 961 ccactcctgc agttcggggg catggatgcg cggatagccg ctgctggttt cctggatgcc 1021 gacggatttg cactgccggt agaactccgc gaggtcgtcc agcctcaggc agcagctgaa 1081 ccaactcgcg aggggatcga gcccctgctg agcctcgaca tgttgtcgca aaattcgccc 1141 tggacccgcc caacgatttg tcgtcactgt caaggtttga cctgcacttc atttggggcc 1201 cacatacacc aaaaaaatgc tgcataattc tcggggcagc aagtcggtta cccggccgcc 1261 gtgctggacc gggttgaatg gtgcccgtaa ctttcggtag agcggacggc caatactcaa 1321 cttcaaggaa tctcacccat gcgcgccggc ggggaaccgg agttcccttc agtgaacgtt 1381 attagttcgc cgctcggtgt gtcgtagata ctagcccctg gggccttttg aaatttgaat 1441 aagatttatg taatcagtct tttaggtttg accggttctg ccgctttttt taaaattgga 1501 tttgtaataa taaaacgcaa ttgtttgtta ttgtggcgct ctatcataga tgtcgctata 1561 aacctattca gcacaatata ttgttttcat tttaatattg tacatataag tagtagggta 1621 caatcagtaa attgaacgga gaatattatt cataaaaata cgatagtaac gggtgatata 1681 ttcattagaa tgaaccgaaa ccggcggtaa ggatctgagc tacacatgct caggtttttt 1741 acaacgtgca caacagaatt gaaagcaaat atcatgcgat cataggcgtc tcgcatatct 1801 cattaaagca gggggtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc 1861 cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa 1921 tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc 1981 ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg 2041 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat 2101 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga 2161 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg 2221 tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg 2281 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc 2341 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat 2401 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa 2461 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg 2521 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata 2581 gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa 2641 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct 2701 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca 2761 atccatcttg ttcaatccac atgatcagat ctctaggcgc gtgggtgcgg acgtagtcag 2821 cgccattgcc gatcgcgtga agttccgccg caaggccgct ggacccagat cctttacagg 2881 aaggccaacg gtggcgccca agaaggattt ccgcgacacc gagaccaata gcggaagccc 2941 caacgccgac ttcagctttt gaaggttcga cagcacgtgc agcgatgttt ccggtgcggg 3001 gctcaagaaa aatcccatcc ccggatcgag gatgagccgg tcggcagcga ccccgctccg 3061 tcgcaaggcg gaaacccgcg cctcgaagaa ccgcacaatc tcgtcgagcg cgtcttcggg 3121 tcgaaggtga ccggtgcggg tggcgatgcc atcccctgcg ctgagtgcat aaccaccagc 3181 ctgcagtccg cctcagcaat atcgggatag agcgcagggt caggaaatcc ttggatatcg 3241 ttcaggtagc ccacgccgcg cttgagcgct agcgcgggtt tccggttgga agctgtcgat 3301 tgaaacacgg tgcatctgat cggacagggc gtctaagagc ggcgcaatac gtctgatctc 3361 atcggccggc gatacaggcc tcgcgtccgg atggctggcg gccggtccga catccacgac 3421 gtctgatccg actcgcagca tttcgaccgc cgcggtgaca gcgttggtgg ggtctagcag 3481 tacgtcaatc gaagaaggag tcctcggtga gattcagaat gccgaacacc gtcaccatgg 3541 cgtcggcctc cgcagcgact tccacgatgg ggatcgggcg agcaaaaagg cagcaattat 3601 gagccccata cctacaaagc cccacgcatc aagcttttga ccctgaagca actaggcaat 3661 ggctgtaatt atgacgacgc cgagtcccga accagactgc ataagcaaca accgacagaa 3721 tggatttcga aaccagagaa agaaaataaa tgcgatgcca taaccgatta tgaacaacgg 3781 cggaaggggc aagcttagta aatgcctcgc tagattttaa tgcggatgtt gcgattactt 3841 cgccaactat tgcgataaca agaaaaagcc agcctttcat gatatatctc ccaatttgtg 3901 tagggcttat tatgcacgct taaaaataat aaaagcagac ttgacctgat agtttggctg 3961 tgagcaatta tgtgcttagt gcatctaatc gcttgagtta acgccggcga agcggcgtcg 4021 gcttgaacga attgttagac attatttgcc gactaccttg gtgatctcgc ctttcacgta 4081 gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt cttgtccaag 4141 ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc gctccattgc 4201 ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt accaaatgcg 4261 ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg agttccatag 4321 cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat caaagagttc 4381 ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca gcaagatagc 4441 cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat tgcgctgcca 4501 ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac 4561 aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc 4621 caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac 4681 cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg 4741 tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg 4801 agtcgatact tcggcgatca ccgcttccct catgatgttt aactttgttt tagggcgact 4861 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg 4921 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacatg tcataacaag 4981 aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc 5041 agttgcgtga ggccatacgc tacttgcatt acagcttacg aaccgaacag gcttatgtcc 5101 actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca 5161 gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg gtctccacgc 5221 atcgtcaggc attggcggcc ttgctgttct tctacggcaa gtgctgtgca cggatctgcc 5281 ctggcttcag gagatcggaa gacctcggcc gtccgggcgc ttgccggtgg tgctgacccc 5341 ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagct 5401 tctgtatgga acgggcatgc ggatcagtga gggtttgcaa ctgcgggtca aggactggat 5461 ttcgatcacg gcacgatcat cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg 5521 ttacccgaga gcttggcacc cagcctgcgc gagcagctgt ctcgtgcacg ggcatggtgg 5581 ctgaaggact aggccgaggg ccgcagcggc gttgcgcttc ccgacgccct tgagcggaag 5641 tatccgcgcg ccgggcattc ctggccgtgg ttctgggttt ttgcgcagca cacgcattcg 5701 accgatccac ggagcggtgt cgtgcgtcgc catcacatgt atgaccagac ctttcagcgc 5761 gccttcaaac gtgccgtaga acaagcaggc atcacgaagc ccgccacacc gcacaccctc 5821 cgccactcgt tcgcgacggc cttgctccgc agcggttacg acattcgaac cgtgcaggat 5881 ctgctcggcc attccgacgt ctctacgacg atgatttaca cgcatgtgct gaaagttggc 5941 ggtgccggag tgcgctcacc gcttgatgcc tgccgcccct cactgtgaga ggtagggcag 6001 cgcaagtcaa tcctagcgga ttcactaccc ctgcgcgaag gccatcggtg ccgcatcgaa 6061 cggccggttg cggaaagtcc tccctgcgtc cgctgatggc cggcagcagc ccgtcgttga 6121 aggatccctg aaagcgacgt tggatgttaa catctacaaa ttgccttttc ttacgaccat 6181 gtacgtaagc gcttacgttt ttggtggacc cttgaggaaa ctggtagctg ttgtgggcct 6241 gtggtctcaa gatggatcat taatttccac cttcacctac gatggggggc atcgcaccgg 6301 tgagtaatat tgtacggcta agagcgaatt tggcctgtag acctcaattg cgagctttct 6361 aatttcaaac tattcgggcc taacttttgg tgtgatgatg ctgactggca ggatatatac 6421 cgttgtaat //