GenBank-Updates@genbank.bio.net (08/28/90)
LOCUS STAREPNA 1143 bp ds-DNA BCT 28-AUG-1990
DEFINITION S.aureus plasmid pCW7 REP N protein (rep N) gene, complete cds.
ACCESSION J03323
KEYWORDS DNA binding protein; REP N protein;
site-specific nicking/closing enzyme.
SOURCE S.aureus plasmid pCW7 DNA.
ORGANISM Staphylococcus aureus
Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
Micrococcaceae.
REFERENCE 1 (bases 1 to 1143)
AUTHORS Balson,D.F. and Shaw,W.V.
TITLE Nucleotide sequence of the REP gene of Staphylococcal plasmid pCW7
JOURNAL Plasmid (1990) In press
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by D.F.Balson, 12-JUL-1990.
FEATURES from to/span description
pept 199 1143 REP N protein (rep N)
site 23 109 RNA countertranscript
signal 117 122 -10 region
signal 140 144 -35 region
binding 182 189 ribosome binding site
site 252 318 pCW7 origin
BASE COUNT 450 a 137 c 193 g 363 t
ORIGIN
1 ttatattaat aaacaaataa aaagaagtcg ctcacgcccc taccaaagtt ttgtgaatgc
61 gacttaccta aataaaatat aaagagttat gttttattaa tttttgtata tctagatatt
121 aaacgatata ggttttattc ttcaagatat atatttgggt gagcgacttc ttaaagtaaa
181 ttaaggagtc gattttttat gagtaaaaat aatcacgcaa atcattcaaa tcatttagaa
241 aatcacgatt tagacaattt ttctaaaacc ggctactcta atagccggtt aaaccgacat
301 actatgtaca ccccggaacc aaaattaagt tttgacgcta tgactattgt tggaaatctt
361 aataaaaata atgctcacaa actatctgaa tttatgagtg tcgagccaca aattcgactt
421 tgggatatac tacaaactaa atttaaagct aaagctctac aagaaaaggt ttatatcgaa
481 tatgacaaag taaaagcaga tacgtgggat agacgtaata tgcgtgttga atttaatcca
541 aataaactta cgcatgaaga aatgctttgg ttaaaacaaa acattatcga ctacatggaa
601 gacgatggtt ttacaagatt agatttagct tttgattttg aatatgattt aagtgattat
661 tatgcaatga ctgataaatc agttaagaaa actatttttt atggtcgtaa cggtaaacca
721 gaaacgaaat attttggtgt tcgtgacagt gatagattta ttagaattta taataaaaaa
781 caggaacgca aagataatgc agatattaaa attatgtctg aacacttatg gcgtgtagaa
841 attgaattaa aaagagatat ggttgattat tggaacgatt gttttaatga tttacatata
901 ttacaaccag attggaaaac tatcgaacgt acttctgata gagcaatggt ttttatgttg
961 ttgaatgatg aagaagaatg gggaaaatta gaaagacgta ctaagaataa atataaaaaa
1021 ttaattaaag aaatatctct aattgattta actgatttaa tgaaatcgac tttaaaagcg
1081 aacgaaaaac aattgcaaaa gcagattgat ttttggcaac gtgaatttag attttggaag
1141 taa
//