GenBank-Updates@genbank.bio.net (08/30/90)
LOCUS PSEAAC6II 1507 bp ds-DNA BCT 30-AUG-1990
DEFINITION P.aeruginosa 6'-N-acetyltransferase (AAC (6')-II) gene, complete
cds.
ACCESSION M29695
KEYWORDS acetyltransferase; gentamicin resistance; netilmicin resistance;
tobramycin resistance.
SOURCE P.aeruginosa (isolate SCH80090884) DNA, clones pSCH[884,1003].
ORGANISM Pseudomonas aeruginosa
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Pseudomonadaceae.
REFERENCE 1 (bases 1 to 1507)
AUTHORS Shaw,K.J., Cramer,C.A., Rizzo,M., Mierzwa,R., Gewain,K.,
Miller,G.H. and Hare,R.S.
TITLE Isolation, characterization and DNA sequence analysis of an AAC
(6')-II gene from Pseudomonas aeruginosa
JOURNAL Antimicrob. Agents Chemother. 33, 2052-2062 (1989)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by K.J.Shaw, 03-NOV-1989.
FEATURES from to/span description
pept 707 1261 6'-N-acetyltransferase
ORF 544 774 ORF A
signal 430 436 -35 region (put.)
signal 458 463 -10 region (put.)
binding 534 539 ribosomal binding site
site 678 683 Tn 21 recombinational hotspot
site 1256 1314 Tn 21 recombinational hotspot
BASE COUNT 370 a 426 c 407 g 304 t
ORIGIN
1 ctcgggtaac atcaaggccc gatccttgga gcccttgccc tcccgcacga tgatcgtgcc
61 gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc tcactgatcc gcatgcccgt
121 tccatacaga agctgggcga acaaacgatg ctcgccttcc agaaaaccga ggatgcgaac
181 cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc ttccgatctc
241 ctgaagccag ggcagatccg tgcacagcac cttgccgtag aagaacagca aggccgccaa
301 tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg acagaaatgc
361 ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac gatgaaggca
421 cgaacccagt ggacataagc ctgttcggtt cgtaagctat aatgcaagta gcgtatgcgc
481 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
541 ttcatggctt gttatgactg tttttttgta cagtctatgc ctcgggcatc caagcagcaa
601 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
661 ggcagtcgcc ctaaaacaaa gttaggcagc acagagcgcc catttcatgt ccgcgagcac
721 cccccccata actcttcgcc tcatgaccga gcgcgacctg ccgatgctcc atgactggct
781 caaccggccg cacatcgttg agtggtgggg tggcgacgaa gagcgaccga ctcttgatga
841 agtgctggaa cactacctgc ccagagcgat ggcggaagag tccgtaacac cgtacatcgc
901 aatgctgggc gaggaaccga tcggctatgc tcagtcgtac gtcgcgctcg gaagcggtga
961 tggctggtgg gaagatgaaa ctgatccagg agtgcgagga atagaccagt ctctggctga
1021 cccgacacag ttgaacaaag gcctaggaac aaggcttgtc cgcgctctcg ttgaactact
1081 gttctcggac cccaccgtga cgaagattca gaccgacccg actccgaaca accatcgagc
1141 catacgctgc tatgagaagg caggattcgt gcgggagaag atcatcacca cgcctgacgg
1201 gccggcggtt tacatggttc aaacacgaca agccttcgag agaaagcgcg gtgttgccta
1261 acaactcatt caagccgacg ccgcttcgcg gcgcggctta attcaggcgt tagatgcact
1321 aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc
1381 gtgcataata agccctacac aaattgggag atatatcatg aaaggctggc tttttcttgt
1441 tatcgcaata gttggcgaag taatcgcaac atccgcatta aaatctagcg agggctttac
1501 taagctt
//