GenBank-Updates@genbank.bio.net (08/30/90)
LOCUS RK2KORB 3528 bp ds-DNA BCT 30-AUG-1990
DEFINITION Plasmid RK2 korB, korFI, korFII and kfrA genes, complete cds; and
incC gene, 3' end.
ACCESSION Y00448 M36479 M38028
KEYWORDS kfrA gene; korB gene; korFI gene; korFII gene; plasmid; repressor;
transcriptional repressor.
SOURCE Plasmid RK2 (from E.coli and P.aeruginosa) DNA.
ORGANISM Plasmid RK2
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 1451)
AUTHORS Thomas,C.M.
JOURNAL Unpublished (1987)
STANDARD simple automatic
REFERENCE 2 (bases 1 to 1451)
AUTHORS Theophilus,B.D.B. and Thomas,C.M.
TITLE Nucleotide sequence of the transcriptional repressor gene KorB
which plays a key role in regulation of the copy number of broad
host range plasmid RK2
JOURNAL Nucleic Acids Res. 15, 7443-7450 (1987)
STANDARD simple automatic
REFERENCE 3 (bases 1085 to 2328)
AUTHORS Jagura-Burdzy,G., Ibbotson,J.P. and Thomas,C.M.
TITLE The korF region of broad host range plasmid RK2 encodes two
polypeptides with transcriptional activity
JOURNAL Unpublished (1990)
STANDARD full staff_entry
REFERENCE 4 (bases 2329 to 3528)
AUTHORS Thomas,C.M., Theophilus,B.D.M., Johnston,L., Jagura-Burdzy,G.,
Schilf,W., Lurz,R. and Lanka,E.
TITLE Identification of a seventh operon on plasmid RK2 regulated by the
korA gene product
JOURNAL Gene 89, 29-35 (1990)
STANDARD simple staff_entry
COMMENT EMBL features not translated to GenBank features:
key from to description
SITE 617 676 put. DNA binding site
Draft entry and computer-readable sequence for [3] kindly submitted
by C.M.Thomas, 12-JUL-1990.
Author address: C.M.Thomas
University of Birmingham
School of Biological Sciences
P.O. Box 363
Birmingham B15 2TT
ENGLAND
FEATURES from to/span description
pept < 1 110 incC gene product (AA at 3)
pept 107 1183 korB gene product
pept 1254 1775 korFI gene product
pept 1768 2295 korFII gene product
pept 2480 3406 krfA gene product
mRNA 1085 2363 korF mRNA
mRNA 2460 > 3406 kfrA mRNA
mRNA 2413 3506 kfrA mRNA (alt.)
signal 2425 2454 kfrAp 1o promoter
signal 2364 2392 kfrAp 2o promoter
site 2421 2432 KorA regulatory protein operator
site 2374 2392 KorB regulatory protein operator
site 2441 2464 KfrA regulatory protein operator
BASE COUNT 750 a 1113 c 1185 g 480 t
ORIGIN
1 catccggtgt gccggtctgg aagatcaaga aaacggccgc gcgcaaggca tcgaaagagg
61 ttcgcgccct ggctgattac gtgttcacga agatggagat ttcccaatga ctgcggctca
121 agccaagacc accaagaaaa acaccgctgc ggccgctcag gaagccgcag gcgcggcgca
181 gccgtccggc ctggggttgg atagcatcgg cgacctgtcg agcctcctgg acgctcctgc
241 ggcgtctcag ggcggttccg gccctatcga gctggacctg gacctgatcg acgaagatcc
301 gcatcagccg cggacggccg acaaccccgg cttttccccg gagagcatcg cggaaatcgg
361 tgccacgatc aaagagcgcg gggtgaagtc acccatttcg gtgcgcgaga accaggagca
421 gccgggccgc tatatcatca atcacggcgc ccgccgctac cgtggctcga agtgggccgg
481 caagaagtcc atcccggcgt tcatcgacaa cgactacaac gaagccgacc aggttatcga
541 gaacctgcaa cgcaacgagc tgaccccgcg cgaaattgcc gacttcattg gccgcgagct
601 ggcgaagggc aagaagaaag gcgatatcgc caaggaaatc ggcaagtcgc cggcgttcat
661 cacccagcac gtcacgctgc tggacctgcc ggagaagatc gccgatgcgt tcaacaccgg
721 ccgcgtgcgc gacgtgaccg tggtgaacga gctggtgacg gccttcaaga agcgcccgga
781 ggaagtcgag gcgtggcttg acgacgacac ccaggaaatc acgcgcggca cggtcaagct
841 gctgcgcgag ttcctggacg agaagggccg cgatcccaac accgtcgatg ccttcaacgg
901 ccagactgat gccgagcgtg acgcggaggc cggcgacggc caggacggcg aggacggcga
961 ccaggacggt aaggacgcca aggaaaaggg cgcgaaggag ccggacccgg acaagctgaa
1021 aaaggccatc gtccaggtcg agcacgacga gcgccctgcc cgccttatcc tcaaccgtcg
1081 gccgccggcg gaaggctatg cctggttgaa gtacgaggac gacggccagg agttcgaggc
1141 gaaccttgcc gacgtgaaac tggtcgcgct catcgagggc tgatccccaa agacagcggc
1201 gcgggccacc cgcgccgcac agacaacggt tccgctacaa ggaggaccga agaatgaatc
1261 cgatgctgtt ctacatcgcg ggaggcgtag gcgcggcgtt gctgctggtt tccgcgatca
1321 tgctgttcaa gctgcgcgag ccgaagaagg aacaccgacc gcagcgcaag gcggcggccc
1381 cgacgccgca gccggtcgat aacgagctgc tgcgcacctt tcgccggccg tcgcaggcca
1441 gccaacccga gccatccacg acgccgccgg cgcggcgcac tgccccgccg agcagcgaac
1501 aatccagagg gaatgacggc gatttcgtca cgtcggccct ggtggccggc ggcgaccaac
1561 agcaccatgc tgggctacct ggccggcggc tcgctgaccg gggccatgct gggcgatgcg
1621 ctgcggccgg atacgccgtc tgcggcgttc gcggacccct cgccgtgctt cggcagcagc
1681 gacacggaca gcggctggac cgataccggc agctcttgcg atttcagttc gtccgacacc
1741 gatacgaaca actggtgagg gcggaccatg actgacgaac aaaagaaacg gctcctgatg
1801 ttgcaagcta tccggcgcag catccaggca atgcgcaggg gtgtttcggg gccggatagt
1861 ttcaagggct ggttgaccgg catcggcatc atcgcggcgg ggttcctggt tggcgagctg
1921 ctgccatccc ctgcccccgg cgtcggcctg gttgtgccag gggcctgcat tgccggcttc
1981 gtcgtcggct gctggctcaa caggaaaggc cgctcatggc cgacagccat ttacgaccaa
2041 ttggcccgtt accagccgct caacgagcgg gcgtaccgcg acctgcaaga gacggtgaag
2101 gaacgcggcc tcgactgcga tgcggtcatg gagtggacct acatcgagca ggccgagttc
2161 atgcccctgg tgcccaccag ggaggaccta gcgcgcgaca agttcctgac cgcgacgcgg
2221 gccgataggg ccgaggacga tgcgcgtgcg cgcttcgtcg tgctccatcc caagaagcgc
2281 ggccccgacg catgaagaag cccgacgccc gctaatcccc ttcccgctga ccctttgccc
2341 ccgccgaccg tgcggggctt ttttgcctgc ccttcagccg ctaaaaaacc tgccgttaag
2401 tcgagaactg ttcagctaaa ctcttgcgct gataaggtag gtaagagtat tattattctt
2461 accaattacc ggagccaaca tggaactgaa caaagagacg cgcgaccgca tttttgcggc
2521 tgcggacgag ctgttcgagc aaggcgaccg ggagaacttc ccgaccgtgg atgcagtgcg
2581 taaggccgcc cgcgtcaaca tgaacgacgc cagcgccggc atgcgcgaat ggcgacgcca
2641 gcgcacggcg caggccgccc cgctggcagt ccaggtgccg gacgcggtgc agcaggccgg
2701 caaccaggcc gtggccgccc tctggcaagc cgcccaggcc cttgccaatg aatcgttgca
2761 ggcagcgcaa gccggctggg atcgtgagcg caacgagttg gaagccgtgc gcggggagct
2821 ggccgacgcc ttcgaggcgc aggcccgcga gctggaagaa gcccaggcgc gcgttacatt
2881 gctagagcag caggccgccg aggcggccga gctggccgca cgccagcggc aagccctggc
2941 cgaagcacgc accgccctgg caggagccga gcagcgggcc gcgctggcga cgcagcgggc
3001 cgacgaggtg gagcgtcgag cgcaggagct gcgcgccgag ctggactacg ctcaccagga
3061 cgcccgcgca ttcaaagaag agagccgcaa gaccctggac gcggccaacc aggaaatgca
3121 ggcgcttcgc ggcgagcgcg acgcgaccag gcgcgaagcg gagagtttgc gcacggagtt
3181 ggcagcggtg aaagcgaagg cgcaggccga cgccgaggcc caccaggagc aaaggaagtt
3241 ggcggcgcaa gaggcggcca ggcaggccga gcgctttacg acggtgcaag ccgagcgtga
3301 cgaagcgcgg caagaggcaa aggcagccca agaagaggcc gctatgttga ggggtcggct
3361 agacgcggaa acgagtggga ggaaacacaa agcgggagga aagtaactga taaatcgaac
3421 gcagttcact aaaatagatg gaacaacctt gacgcgcccg gctctaggga aagccggcct
3481 cgaactgccg aggtcggctt ttttttagcc ggcaagcgac caggacac
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