GenBank-Updates@genbank.bio.net (08/30/90)
LOCUS ECORRD 1541 bp ss-rRNA RNA 30-AUG-1990
DEFINITION E.coli 16S ribosomal RNA.
ACCESSION J01859 M24828 M24911 K02555 M24996 M24833 M24834 M24835 M24836
M24837
KEYWORDS 16S ribosomal RNA; ribosomal RNA.
SOURCE Escherichia coli rRNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1541)
AUTHORS Ehresmann,C., Stiegler,P., Carbon,P. and Ebel,J.-P.
TITLE Recent progress in the determination of the primary sequence of the
16 S RNA of Escherichia coli
JOURNAL FEBS Lett. 84, 337-341 (1977)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 1541)
AUTHORS Carbon,P., Ehresmann,C., Ehresmann,B. and Ebel,J.-P.
TITLE The sequence of Escherichia coli ribosomal 16 S RNA determined by
new rapid gel methods
JOURNAL FEBS Lett. 94, 152-156 (1978)
STANDARD full staff_review
REFERENCE 3 (bases 1 to 1541)
AUTHORS Carbon,P., Ehresmann,C., Ehresmann,B. and Ebel,J.-P.
TITLE The complete nucleotide sequence of the ribosomal 16-S RNA from
Escherichia coli: Experimental details and cistron heterogeneities
JOURNAL Eur. J. Biochem. 100, 399-410 (1979)
STANDARD full staff_review
REFERENCE 4 (bases 1346 to 1541)
AUTHORS Taylor,B.H., Prince,J.B., Ofengand,J. and Zimmermann,R.A.
TITLE Nonanucleotide sequence from 16S ribonucleic acid at the peptidyl
transfer ribonucleic acid binding site of the Escherichia coli
ribosome
JOURNAL Biochemistry 20, 7581-7588 (1981)
STANDARD full staff_review
REFERENCE 5 (bases 1442 to 1541)
AUTHORS Schnare,M.N. and Gray,M.W.
TITLE 3'-terminal sequence of wheat mitochondrial 18S ribosomal RNA:
Further evidence of a eubacterial evolutionary origin
JOURNAL Nucleic Acids Res. 10, 3921-3932 (1982)
STANDARD full staff_review
REFERENCE 6 (bases 1 to 1541)
AUTHORS Ehresmann,C., Stiegler,P., Fellner,P. and Ebel,J.-P.
TITLE The determination of the primary structure of the 16s ribosomal rna
of escherichia coli: III. Further studies
JOURNAL Biochimie 57, 711-748 (1975)
STANDARD simple staff_entry
REFERENCE 7 (bases 1 to 1541)
AUTHORS Ehresmann,C., Stiegler,P., Fellner,P. and Ebel,J.-P.
TITLE the determination of the primary structure of the 16S ribosomal RNA
of Escherichia coli: (2) nucleotide sequences of products from
partial enzymatic hydrolysis
JOURNAL Biochimie 54, 901-967 (1972)
STANDARD simple staff_entry
REFERENCE 8 (sites)
AUTHORS Zimmermann,R.A. and Singh-Bergmann,K.
TITLE Binding sites for ribosomal proteins S8 and S15 in the 16S RNA of
Escherichia coli
JOURNAL Biochim. Biophys. Acta 563, 422-431 (1979)
STANDARD simple staff_entry
REFERENCE 9 (bases 558 to 890)
AUTHORS Gregory,R.J., Zeller,M.L., Thurlow,D.L., Gourse,R.L., Stark,M.J.R.,
Dahlberg,A.E. and Zimmermann,R.A.
TITLE Interaction of ribosomal proteins S6, S8, S15 and S18 with the
central domain of 16S ribosomal RNA from Escherichia coli
JOURNAL J. Mol. Biol. 178, 287-302 (1984)
STANDARD simple staff_review
REFERENCE 10 (bases 16 to 86; 147 to 246; 281 to 772)
AUTHORS Ungewickell,E., Garrett,R., Ehresmann,C., Stiegler,P. and
Fellner,P.
TITLE An investigation of the 16-S RNA binding sites of ribosomal
proteins S4, S8, S15 and S20 from Escherichia coli
JOURNAL Eur. J. Biochem. 51, 165-180 (1975)
STANDARD simple staff_entry
COMMENT [2], [3] contain updated sequence data for the original work by the
same laboratory [1]. There were too many discrepancies between [1]
and [2], [3] to list each revision in our sites table. The sequence
shown is from [3]. [1], [2], [3] point to a number of cistron
heterogeneities. There is uncertainty, however, with regard to
assigning these various heterogeneities to specific cistrons. The
RNA method used by [1], [2], [3] gives the average of all the
cistrons present in the cell [3]. The heterogeneities are
classified by their relative proportions into major, minor and
undetermined species. The sequence shown corresponds to the major
species. The heterogeneities were annotated as variations in the
sites table. It is not known which of the residues "c" (base 633)
or "a" (base 641) undergoes a deletion, giving rise to the minor
component "atctg". [3] suggests the existence of one or two mutated
cistrons among the known seven cistrons of ribosomal RNA.
With the exception of a single base deletion, this sequence is
identical to the current 16S rDNA sequence for the E.coli rRNB
gene.
FEATURES from to/span description
rRNA 1 1541 16S rRNA
variant 76 76 g in major species; c in undetermined species
variant 80 80 a in major species; c in undetermined species
variant 88 89 tc in major species; tgc in undetermined
species
variant 90 92 ttt in major species; substitution of one of
the t by c in undetermined species
variant 179 179 t in major species; c in minor species
variant 182 182 c in major species 1; t in major species 2
variant 193 194 ca in major species; cga in undetermined
species
variant 267 272 tcacct in major species; ccacct or tcaccc in
minor species
variant 283 285 ccc in major species; cc in minor species
conflict 348 349 ag in [3]; ga in [2]
modified 526 526 m7g = 7-methylguanosine
variant 630 631 ct in major species; cct in minor species
variant 632 634 gca in major species; ga in minor species (see
comment)
variant 640 642 tac in major species; tc in minor species (see
comment)
conflict 853 855 ttc in [3]; ctt in [2]
revision 915 916 tg in [3]; tcg in [1],[2] (typing error)
variant 965 965 g in undetermined species 1; 2-methylguanosine
in species 2
conflict 1027 1029 ctt in [3]; ttc in [2]
variant 1071 1072 gt in major species; gtt in undetermined
species
variant 1074 1074 t in major species; a in undetermined species
modified 1099 1099 m5c = 5-methylcytidine
modified 1206 1206 m2g = 2-methylguanosine
variant 1280 1280 c in major species; t in minor species
variant 1320 1322 tcg in major species; tg in minor species
modified 1401 1401 m4c = n4-methylcytidine
modified 1402 1402 cm = 2'-o-methylcytidine
variant 1405 1405 t in major species; c in minor species
variant 1406 1406 5-methylcytidine in major species; c in minor
species
conflict 1490 1493 gaag in [3]; gag in [4]
modified 1497 1497 um = 2'-o-methyluridine
modified 1517 1517 m66a = n6,n6-dimethyladenosine
modified 1518 1518 m66a = n6,n6-dimethyladenosine
BASE COUNT 389 a 352 c 487 g 313 t
ORIGIN 5' end of mature rRNA.
1 aaattgaaga gtttgatcat ggctcagatt gaacgctggc ggcaggccta acacatgcaa
61 gtcgaacggt aacaggaaga agcttgctct ttgctgacga gtggcggacg ggtgagtaat
121 gtctgggaaa ctgcctgatg gagggggata actactggaa acggtagcta ataccgcata
181 acgtcgcaag accaaagagg gggaccttcg ggcctcttgc catcggatgt gcccagatgg
241 gattagctag taggtggggt aacggctcac ctaggcgacg atccctagct ggtctgagag
301 gatgaccagc cacactggaa ctgagacacg gtccagactc ctacgggagg cagcagtggg
361 gaatattgca caatgggcgc aagcctgatg cagccatgcc gcgtgtatga agaaggcctt
421 cgggttgtaa agtactttca gcggggagga agggagtaaa gttaatacct ttgctcattg
481 acgttacccg cagaagaagc accggctaac tccgtgccag cagccgcggt aatacggagg
541 gtgcaagcgt taatcggaat tactgggcgt aaagcgcacg caggcggttt gttaagtcag
601 atgtgaaatc cccgggctca acctgggaac tgcatctgat actggcaagc ttgagtctcg
661 tagagggggg tagaattcca ggtgtagcgg tgaaatgcgt agagatctgg aggaataccg
721 gtggcgaagg cggccccctg gacgaagact gacgctcagg tgcgaaagcg tggggagcaa
781 acaggattag ataccctggt agtccacgcc gtaaacgatg tcgacttgga ggttgtgccc
841 ttgaggcgtg gcttccggag ctaacgcgtt aagtcgaccg cctggggagt acggccgcaa
901 ggttaaaact caaatgaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt
961 cgatgcaacg cgaagaacct tacctggtct tgacatccac ggaagttttc agagatgaga
1021 atgtgccttc gggaaccgtg agacaggtgc tgcatggctg tcgtcagctc gtgttgtgaa
1081 atgttgggtt aagtcccgca acgagcgcaa cccttatcct ttgttgccag cggtccggcc
1141 gggaactcaa aggagactgc cagtgataaa ctggaggaag gtggggatga cgtcaagtca
1201 tcatggccct tacgaccagg gctacacacg tgctacaatg gcgcatacaa agagaagcga
1261 cctcgcgaga gcaagcggac ctcataaagt gcgtcgtagt ccggattgga gtctgcaact
1321 cgactccatg aagtcggaat cgctagtaat cgtggatcag aatgccacgg tgaatacgtt
1381 cccgggcctt gtacacaccg cccgtcacac catgggagtg ggttgcaaaa gaagtaggta
1441 gcttaacctt cgggagggcg cttaccactt tgtgattcat gactggggtg aagtcgtaac
1501 aaggtaaccg taggggaacc tgcggttgga tcacctcctt a
//