GenBank-Updates@genbank.bio.net (08/30/90)
LOCUS PLPPSASPR 1447 bp ds-DNA BCT 30-AUG-1990
DEFINITION Inc W plasmid pSa streptomycin-spectinomycin-resistance gene
(Sp-R), complete cds, and kanamycin-resistance, 3' end.
ACCESSION M11444
KEYWORDS kanamycin-resistance; spectinomycin-resistance;
streptomycin-resistance.
SOURCE Inc W plasmid pSa DNA.
ORGANISM Plasmid pSa
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 1447)
AUTHORS Tait,R.C., Rempel,H., Rodriguez,R.L. and Kado,C.I.
TITLE The aminoglycoside-resistance operon of the plasmid pSa: Nucleotide
sequence of the streptomycin-spectinomycin resistance gene
JOURNAL Gene 36, 97-104 (1985)
STANDARD simple staff_entry
FEATURES from to/span description
pept 378 1187 streptomycin-spectinomycin-resistance protein
(Sp-R)
pept < 1 327 kanamycin-resistance (Km-R) (AA at 1)
BASE COUNT 370 a 362 c 388 g 327 t
ORIGIN
1 agtcgtacgt tgctcttgga acggggacga tggtgggaag aagaaaccga tccaggagta
61 cgcggaatag accagtcact ggcgaatgca tcacaactgg gcaaaggctt gggaaccaag
121 ctggttcgag ctctggttga gttgctgttc aatgatcccg aggtcaccaa gatccaaacg
181 gacccgtcgc cgagcaactt gcgagcgatc cgatgctacg agaaagcggg gtttgagagg
241 caaggtaccg taaccacccc agatggtcca gccgtgtaca tggttcaaac acgccaggca
301 ttcgagcgaa cacgcagtga tgcctaaccc ttccatcgag ggggacgtcc aagggctggc
361 gcccttggcc gcccctcatg tcaaacgtta gacatcatga gggtagcgtg accatcgaaa
421 tttcgaacca actatcagag gtgctaagcg tcattgagcg ccactctgga atcaacgttg
481 ctggccgtgc atttgtacgc tccgcagtgg atggcggcct gaagccatac agcgatattg
541 atttgttggt tactgtggcc gtaaagcttg atgaaacgac gcggcgagca ttgctcaatg
601 accttatgga ggcttcggct ttccctggcg agagcgagac gctccgcgct atagaagtca
661 cccttgtcgt gcatgacgac atcatcccgt ggcgttatcc ggctaagcgc gagctgcaat
721 ttggagaatg gcagcgcaat gacattcttg cgggtatctt cgagccagcc atgatcgaca
781 ttgatctagc tatcctgctt acaaaagcaa gagaacatag cgttgccttg gtaggtccgg
841 cagcggagga attctttgac ccggttcctg aacaggatct attcgaggcg ctgagggaaa
901 ccttgaagct atggaactcg cagcccgact gggccggcga tgagcgaaat gtagtgctta
961 cgttgtcccg catttggtac agcgcaataa ccggcaaaat cgcgccgaag gatgtcgctg
1021 ccgactgggc aataaaacgc ctacctgccc agtatcagcc cgtcttactt gaagctaagc
1081 aagcttatct gggacaaaaa gaagatcact tggcctcacg cgcagatcac ttggaagaat
1141 ttattcgctt tgtgaaaggc gagatcatca agtcagttgg taaatgatgt ctaacaattc
1201 gttcaaccga ctcatcgcgc cggcggctta actccggcgt tagatgcact aagcacataa
1261 ttgatcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata
1321 agccctacac aaattgggag atatatcatg aaaggctggc ttttcttgtt atcgaatagt
1381 tggcgaagta atcgcaacag ccgaattaaa atctagcagg gctttactaa gcttgcccct
1441 tccgccg
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