GenBank-Updates@genbank.bio.net (08/30/90)
LOCUS BSUHAGFA 2360 bp ds-DNA BCT 30-AUG-1990
DEFINITION B.subtilis flagellin gene (hag), complete cds.
ACCESSION M26948
KEYWORDS H-antigen; flagellar filament; flagellin; motility protein.
SOURCE B.subtilis (strain I168) DNA, clone pDM67.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes;
Endospore-forming rods and cocci; Bacillaceae.
REFERENCE 1 (bases 1 to 2360)
AUTHORS Mirel,D.B. and Chamberlin,M.J.
TITLE The Bacillus subtilis flagellin gene (hag) is transcribed by the
sigma-28 form of RNA polymerase
JOURNAL J. Bacteriol. 171, 3095-3101 (1989)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable copy of sequence [1] kindly
provided by D.B.Mirel, 21-AUG-1989.
FEATURES from to/span description
pept 545 1459 flagellin
signal 420 423 sigma D promoter -35 consensus
signal 439 446 sigma D promoter -10 consensus
binding 529 535 ribosome binding site (put.)
signal 1634 1637 sigma D promoter -35 consensus
signal 1652 1659 sigma D promoter -10 consensus
BASE COUNT 798 a 438 c 493 g 631 t
ORIGIN
1 atcgataata ttcaagacgt agaagtcatg acaatattga ctatggcaga gcatttgaaa
61 agtctactgc gaatttattg gctcccatta ttgtgaatcg caagaacatg atggctaagc
121 aagtcgtttt acacgactcc tcatatacga caaagcatcc gattggagga gaatcatgct
181 agttttatcg cggaaaataa acgaagcgat tcaaataggt gctgatattg aagtaaaagt
241 gattgcggtt gaaggggatc aagtgaagct tggaattgac gccccaaagc atattgatat
301 tcacaggaaa gaaatttact tgaccattca ggaagaaaat aaccgtgcag cagcgttatc
361 cagcgatgtg atctccgcat tatcctcaca aaaaaagtga ggattttttt atttttgtat
421 taacaaaatc agagacaatc cgatattaat gatgtagccg ggaggaggcg caaaagactc
481 agccagttac aaaataaggg cacaaggacg tgccttaaca acatattcag ggaggaacaa
541 aacaatgaga attaaccaca atattgcagc gcttaacaca ctgaaccgtt tgtcttcaaa
601 caacagtgcg agccaaaaga acatggagaa actttcttca ggtcttcgca tcaaccgtgc
661 gggagatgac gcagcaggtc ttgcgatctc tgaaaaaatg agaggacaaa tcagaggtct
721 tgaaatggct tctaaaaact ctcaagacgg aatctctctt atccaaacag ctgagggtgc
781 attaactgaa actcatgcga tccttcaacg tgttcgtgag ctagttgttc aagctggaaa
841 cactggaact caggacaaag caactgattt gcaatctatt caagatgaaa tttcagcttt
901 aacagatgaa atcgatggta tttcaaatcg tacagaattc aatggtaaga aattgctcga
961 tggcacttac aaagttgaca cagctactcc tgcaaatcaa aagaacttgg tattccaaat
1021 cggagcaaat gctacacagc aaatctctgt aaatattgag gatatgggtg ctgacgctct
1081 tggaattaaa gaagctgatg gttcaattgc agctcttcat tcagttaatg atcttgacgt
1141 aacaaaattc gcagataatg cagcagatac tgctgatatc ggtttcgatg ctcaattgaa
1201 agttgttgat gaagcgatca accaagtttc ttctcaacgt gctaagcttg gtgcggtaca
1261 aaatcgtcta gagcacacaa ttaacaactt aagcgcttct ggtgaaaact tgacagctgc
1321 tgagtctcgt atccgtgacg ttgacatggc taaagagatg agcgaattca caaagaacaa
1381 cattctttct caggcttctc aagctatgct tgctcaagca aaccaacagc cgcaaaacgt
1441 acttcaatta ttacgttaat tttaaaaaag accttggcgt tgccagggtc ttttaattta
1501 aatttctatc tcctaatcat tcctcatcct gtcactaact catgatataa taaccggatt
1561 ctccactaac tttttataaa tgtatttcca tacaagaaat ctaaaacaga agattttttt
1621 ccaaaaatat gtgtaatctt atctcgactt agtcgatata aacgatagat tggggcatag
1681 gggatgatca attgaacatt gaaaggctca ctacgttaca acctgtttgg gatcgttatg
1741 atactcaaat acataatcag aaagataatg ataacgaggt tcctgttcat caagtttcat
1801 ataccaatct tgctgaaatg gtgggggaaa tgaacaagct tttggaacct tcgcaagttc
1861 atctgaagtt cgagcttcat gacaagttaa atgaatacta tgtaaaggta atagaggact
1921 ctacaaatga agtgatccgc gaaattccac caaaacggtg gcttgatttt tatgcggcta
1981 tgactgaatt tcttgggtta tttgtagatg aaaaaaagta gaataggagt ggtttgagat
2041 ggtcacaaga ataacaggtc tggcgtcagg aatggatata gatgatatcg tatcaaagct
2101 gatgcagaca gaaagagcgc cgcttgataa gctgacacaa aaaaagcaga ctcttgaatg
2161 gcagcgtgac agctatcgtg aagtaaactc aaaaataaaa gaattgcaag attatatgtc
2221 taaaaatacg ttgacatatc cgagcacgta tcagagcaaa actgtgacaa gctctaatga
2281 gtctgtgtta actgctacag gaagcgtaag tgcgccaaat tcttcgtcta ctgttgaagt
2341 ggcgagcttg gcaactgcag
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