GenBank-Updates@genbank.bio.net (09/02/90)
LOCUS NPAEGT 2793 bp ds-DNA VRL 02-SEP-1990
DEFINITION AcMNPV ecdysteroid UDP-glucosyl transferase (egt) gene, complete
cds.
ACCESSION M22619 M33904
KEYWORDS ecdysteroid UDP-glucosyltransferase.
SOURCE Autographa californica nuclear polyhedrosis virus (strain L-1) DNA.
ORGANISM Autographa californica nuclear polyhedrosis virus
Viridae; ds-DNA enveloped viruses; Baculoviridae; Baculovirus;
Nuclear polyhedrosis viruses.
REFERENCE 1 (sites)
AUTHORS O'Reilly,D.R. and Miller,L.K.
TITLE A baculovirus blocks insect molting by producing ecdysteroid
UDP-glucosyltransferase
JOURNAL Science 245, 1110-1112 (1989)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 1800; for [1])
AUTHORS O'Reilly,D.R. and Miller,L.K.
TITLE Regulation of expression of a baculovirus ecdysteroid UDPglucosyl-
transferase gene
JOURNAL J. Virol. 64, 1321-1328 (1990)
STANDARD full staff_review
REFERENCE 3 (bases 1 to 2793; for [1])
AUTHORS Miller,L.K.
JOURNAL Unpublished (1989)
STANDARD full staff_review
REFERENCE 4 (bases 1600 to 2793; for [1])
AUTHORS O'Reilly,D.R., Passarelli,A.L., Goldman,I.F. and Miller,L.K.
TITLE Characterization of the DA26 gene in a hypervariable region of the
Autographa californica nuclear polyhedrosis virus genome
JOURNAL J. Virol. 71, 1029-1037 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [3] kindly submitted
by L.K.Miller, 22-FEB-1989.
FEATURES from to/span description
pept 149 1669 ecdysteroid UPD-glucosyltransferase
mRNA 106 1695 ecdysteroid UPD-glucosyltransferase mRNA
mRNA 1779 > 2793 DA26 mRNA
mRNA 2031 > 2793 late mRNA
ORF 1815 2492 open reading frame DA26
signal 78 82 TATA box
signal 1677 1682 poly-A signal
signal 1750 1754 TATA box
BASE COUNT 846 a 613 c 592 g 742 t
ORIGIN 1 bp upstream of SalI site; 8.3-10.5 map units.
1 gtcgacgcgc ttctgcgtat aattgcacac taacatgttg ccctttgaac ttgacctcga
61 ttgtgttaat ttttggctat aaaaaggtca ccctttaaaa tttgttacat aatcaaatta
121 ccagtacagt tattcggttt gaagcaaaat gactattctc tgctggcttg cactgctgtc
181 tacgcttact gctgtaaatg cggccaatat attggccgtg tttcctacgc cagcttacag
241 ccaccatata gtgtacaaag tgtatattga agcccttgcc gaaaaatgtc acaacgttac
301 ggtcgtcaag cccaaactgt ttgcgtattc aactaaaact tattgcggta atatcacgga
361 aattaatgcc gacatgtctg ttgagcaata caaaaaacta gtggcgaatt cggcaatgtt
421 tagaaagcgc ggagtggtgt ccgatacaga cacggtaacc gccgctaact acctaggctt
481 gattgaaatg ttcaaagacc agtttgacaa tatcaacgtg cgcaatctca ttgccaacaa
541 ccagacgttt gatttagtcg tcgtggaagc gtttgccgat tatgcgttgg tgtttggtca
601 cttgtacgat ccggcgcccg taattcaaat cgcgcctggc tacggtttgg cggaaaactt
661 tgacacggtc ggcgccgtgg cgcggcaccc cgtccaccat cctaacattt ggcgcagcaa
721 tttcgacgac acggaggcaa acgtgatgac ggaaatgcgt ttgtataaag aatttaaaat
781 tttggccaac atgtccaacg cgttgctcaa acaacagttt ggacccaaca caccgacaat
841 tgaaaaacta cgcaacaagg tgcaattgct tttgctaaac ctgcatccca tatttgacaa
901 caaccgaccc gtgccgccca gcgtgcagta tcttggcgga ggaatccatc ttgtaaagag
961 cgcgccgttg accaaattaa gtccggtcat caacgcgcaa atgaacaagt caaaaagcgg
1021 aacgatttac gtaagttttg ggtcgagcat tgacaccaaa tcgtttgcaa acgagtttct
1081 ttacatgtta atcaatacgt tcaaaacgtt ggataattac accatattat ggaaaattga
1141 cgacgaagta gtaaaaaaca taacgttgcc cgccaacgta atcacgcaaa attggtttaa
1201 tcaacgcgcc gtgctgcgtc ataaaaaaat ggcggcgttt attacgcaag gcggactaca
1261 atcgagcgac gaggccttgg aagccgggat acccatggtg tgtctgccca tgatgggcga
1321 ccagttttac catgcgcaca aattacagca actcggcgta gcccgcgcct tggacactgt
1381 taccgtttcc agcgatcaac tactagtggc gataaacgac gtgttgttta acgcgcctac
1441 ctacaaaaaa cacatggccg agttatatgc gctcatcaat catgataaag caacgtttcc
1501 gcctctagat aaagccatca aattcacaga acgcgtaatt cgatatagac atgacatcag
1561 tcgtcaattg tattcattaa aaacaacagc tgccaatgta ccgtattcaa attactacat
1621 gtataaatct gtgttttcta ttgtaatgaa tcacttaaca cacttttaat tacgtcaata
1681 aatgttattc accattattt acctggtttt tttgagaggg gctttgtgcg actgcgcact
1741 tccagccttt ataaacgctc accaaccaaa gcaggtcatt attgtgccag gacgttcaaa
1801 ggcgaaacat cgaaatggag tctgttcaaa cgcgcttatg tgccagtagc aatcaatttg
1861 ctccgttcaa aaagcgccag cttgccgtgc cggtcggttc tgtgaacagt ttgacacaca
1921 ccatcacctc caccaccgtc accagcgtga ttccaaaaaa ttatcaagaa aaacgtcaga
1981 aaatatgcca cataatatct tcgttgcgta acacgcactt gaatttcaat aagatacagt
2041 ctgtacataa aaagaaactg cggcatttgc aaaatttgct aagaaaaaag aacgaaatta
2101 ttgccgagtt ggttagaaaa cttgaaagtg cacagaagaa gacaacgcac agaaatatta
2161 gtaaaccagc tcattggaaa tactttggag tagtcagatg tgacaacaca attcgcacaa
2221 ttattggcaa cgaaaagttt gtaaggagac gtttggccga gctgtgcaca ttgtacaacg
2281 ccgagtacgt gttttgccaa gcacgcgccg atggagacaa agatcgacag gcactagcga
2341 gtctgctgac ggcggcgttt ggttcgcgag tcatagttta tgaaaatagt cgccggttcg
2401 agtttataaa tccggacgag attgctagtg gtaaacgttt aataattaaa catttgcaag
2461 atgaatctca aagtgatatt aacgcctatt aatttgaaag gtgaggaaga gcccaattgc
2521 gttgagcgca ttaccataat gccatgtatt ttaatagata ctgagatctg tttaaatgtc
2581 agatgccgtt ctccttttgc caaattcaaa gtattgatta ttgtagatgg ctttgatagc
2641 gcttatattc aggctacctt ttgtagcatt agcgatagtg taacaattgt taacaaatct
2701 aacgaaaagc atgtaacgtt tgacgggttt gtaaggccgg acgatgaagg tacaacaatg
2761 ccttatgtca ttggaccatt atattctgtc gac
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