GenBank-Updates@genbank.bio.net (09/02/90)
LOCUS ECOALPHOA 171 bp ds-DNA BCT 02-SEP-1990
DEFINITION Escherichia coli Mu dI insertion site.
ACCESSION M33724
KEYWORDS alkaline phosphatase.
SOURCE E.coli K12 DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 171)
AUTHORS Metcalf,W.W., Steed,P.M. and Wanner,B.L.
TITLE Identification of phosphate-starvation-inducible genes in
Escherichia coli K-12 by DNA sequence analysis of psi::lacZ(Mu dI)
transcriptional fusions
JOURNAL J. Bacteriol. 172, 3191-3200 (1990)
STANDARD full staff_entry
REFERENCE 2 (sites)
AUTHORS Chang,C.N., Kuang,W.-J. and Chen,E.Y.
TITLE Nucleotide sequence of the alkaline phosphatase gene of Escherichia
coli
JOURNAL Gene 44, 121-125 (1986)
STANDARD simple staff_review
COMMENT Authorin copy of sequence for [1] kindly submitted by W.W.Metcalf
13-APR-1990.
FEATURES from to/span description
site 1 141 phoA coding sequence /note="upstream sequence
of psiA44:Mu dI" /evidence=EXPERIMENTAL
[1],[2]
site 141 142 Mu dI insertion site /evidence=EXPERIMENTAL
/note="psiA44:Mu dI insertion site" [1]
trns 142 > 171 Mu dI-R /evidence=EXPERIMENTAL [1]
BASE COUNT 49 a 45 c 51 g 26 t
ORIGIN
1 caaagctccg ggcctcaccc aggcgctaaa taccaaagat ggcgcagtga tggtgatgag
61 ttacgggaac tccgaagagg attcacaaga acataccggc agtcagttgc gtattgcggc
121 gtatggcccg catgccgcca atgaagcggc gcacgaaaaa cgcgaaagcg t
//