GenBank-Updates@genbank.bio.net (09/02/90)
LOCUS ECOALPHOE 97 bp ds-DNA BCT 02-SEP-1990 DEFINITION Escherichia coli Mu dI insertion site. ACCESSION M33727 KEYWORDS alkaline phosphatase. SOURCE E.coli K12 DNA. ORGANISM Escherichia coli Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively anaerobic rods; Enterobacteriaceae. REFERENCE 1 (bases 1 to 97) AUTHORS Metcalf,W.W., Steed,P.M. and Wanner,B.L. TITLE Identification of phosphate-starvation-inducible genes in Escherichia coli K-12 by DNA sequence analysis of psi::lacZ(Mu dI) transcriptional fusions JOURNAL J. Bacteriol. 172, 3191-3200 (1990) STANDARD full staff_entry REFERENCE 2 (sites) AUTHORS Chang,C.N., Kuang,W.-J. and Chen,E.Y. TITLE Nucleotide sequence of the alkaline phosphatase gene of Escherichia coli JOURNAL Gene 44, 121-125 (1986) STANDARD simple staff_review COMMENT Authorin copy of sequence for [1] kindly submitted by W.W.Metcalf 13-APR-1990. FEATURES from to/span description site 1 67 phoA coding sequence /note="upstream sequence of psiA5::Mu dI. psiA30 upstream region is identical" /evidence=EXPERIMENTAL [1],[2] site 67 68 Mu dI insertion site /evidence=EXPERIMENTAL /note="psiA5:Mu dI insertion site; psiA30 upstream region is identical" [1] trns 68 > 97 Mu dI-R /evidence=EXPERIMENTAL [1] BASE COUNT 34 a 13 c 22 g 28 t ORIGIN 1 ttgtttttat tttttaatgt atttgtacat ggagaaaata aagtgaaaca aagcactatt 61 gcactggtga agcggcgcac gaaaaacgcg aaagcgt //