GenBank-Updates@genbank.bio.net (09/02/90)
LOCUS ECOALPHOH 233 bp ds-DNA BCT 02-SEP-1990
DEFINITION Escherichia coli Mu dI insertion site.
ACCESSION M33730
KEYWORDS alkaline phosphatase.
SOURCE E.coli K12 DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 233)
AUTHORS Metcalf,W.W., Steed,P.M. and Wanner,B.L.
TITLE Identification of phosphate-starvation-inducible genes in
Escherichia coli K-12 by DNA sequence analysis of psi::lacZ(Mu dI)
transcriptional fusions
JOURNAL J. Bacteriol. 172, 3191-3200 (1990)
STANDARD full staff_entry
REFERENCE 2 (sites)
AUTHORS Chen,C.-M., Ye,Q.-Z., Zhu,Z., Wanner,B.L. and Walsh,C.T.
TITLE Molecular biology of carbon-phosphorus bond cleavage: Cloning and
sequencing of the phn (psiD) genes involved in alkylphosphonate
uptake and C-P lyase activity in Escherichia coli B
JOURNAL J. Biol. Chem. 265, 4461-4471 (1990)
STANDARD full staff_review
COMMENT Authorin copy of sequence for [1] kindly submitted by W.W.Metcalf
13-APR-1990.
FEATURES from to/span description
site 1 203 phn operon sequence /note="upstream sequence
of psiD31" /evidence=EXPERIMENTAL [1],[2]
site 203 204 Mu dI insertion site /evidence=EXPERIMENTAL
/note="Mu dI insertion site into phnD gene
(psiD31::Mu dI)" [1]
trns 204 > 233 Mu dI-R /evidence=EXPERIMENTAL [1]
variant 201 201 g in E.coli; a in E.coli B
variant 174 174 c in E.coli; g in E.coli B
variant 141 141 g in E.coli; a in E.coli B
variant 135 135 g in E.coli; a in E.coli B
variant 76 76 t in E.coli; c in E.coli B
variant 51 51 g in E.coli; a in E.coli B
BASE COUNT 62 a 64 c 66 g 41 t
ORIGIN
1 tcgctggcct tcaccagcat gttcagcctc agcaccctgt taagcccggc gcacgccgaa
61 gagcaggaaa aggcgttgaa tttcggcatt atttcaacgg aatcacagca aaacctgaaa
121 ccgcaatgga cgccgttctt gcaggatatg gagaagaagc tgggcgtgaa ggtcaacgcc
181 ttctttgccc cggactacgc gggtgaagcg gcgcacgaaa aacgcgaaag cgt
//