GenBank-Updates@genbank.bio.net (09/02/90)
LOCUS ECOPOLBDA 4666 bp ds-DNA BCT 02-SEP-1990
DEFINITION E.coli DNA polymerase (polB) gene, 5' flank.
ACCESSION M35371
KEYWORDS DNA polymerase; polB gene.
SOURCE E.coli (strain W3110) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 4666)
AUTHORS Chen,H., Sun,Y., Stark,T., Beattie,W. and Moses,R.
TITLE Nucleotide sequence and deletion analysis of the polB gene of
E.coli
JOURNAL Unpublished (1990)
STANDARD simple staff_review
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by H.Chen, 20-JUN-1990.
Author address: H.Chen
Baylor College of Medicine
Cell Biology and IMG
One Baylor Plaza
Houston, TX 77030
email: hchen@vulture.bcm.tmc.edu
FEATURES from to/span description
pept 1264 3576 DNA polymerase (polB) (gtg start codon)
signal 1209 1214 -35 region
signal 1229 1234 -10 region
binding 1250 1255 ribosome binding site
site 1193 1208 lexA box
BASE COUNT 1100 a 1278 c 1257 g 1031 t
ORIGIN
1 aagcttgcat gcctgcaggt cgactctaga ggatcctcgc tggtggcgcg caccataccg
61 tcttcagcca tgcactgaac ctcaacgata tgcgccaatt cgccgagatg cacgacattg
121 aaatcacggt gattgataac gacacacgcc tgccagcgtt taaagacgcg ctgcgctgga
181 acgaagtgta ttacgggttt cgtcgctaag tagccgcatc cggtatgtaa cgcctgatgc
241 gacgctgacg cgtcttatct ggcctacacg ctgcgatttt gtaggccgga taagcaaagc
301 gcatccggca ttcaacgcct gatgcgacgc tggcgcgtct tatcaggcct acgcgctgcg
361 attttgtagg ccggataagc aaagcgcatc cggcattcaa cgcctgatgc gacgctggcg
421 cgtcttatca ggcctacacg ctgcgatttt gtaggccgga taagcaaagc gcatccggca
481 cgaaggagtc aacatgttag aagatctcaa acgccaggta ttagaagcca acctggcgct
541 gccaaaacac aacctggtca cgctcacatg gggcaacgtc agcgccgttg atcgcgagcg
601 cggcgtcttt gtgatcaaac cttccggcgt cgattacagc gtcatgaccg ctgacgatat
661 ggtcgtggtt agcatcgaaa ccggtgaagt ggttgaaggt acgaaaaagc cctcctccga
721 cacgccaact caccggctgc tctatcaggc attcccctcc attggcggca ttgtgcatac
781 gcactcgcgc cacgccacca tctgggcgca ggcgggtcag tcgattccag caaccggcac
841 cacccacgcc gactatttct acggcaccat tccctgcacc cgcaaaatga ccgacgcaga
901 aatcaacggc gaatatgagt gggaaaccgg taacgtcatc gtagaaacct ttgaaaaaca
961 gggtatcgat gcagcgcaaa tgcccggcgt tctggtccat tcccacggcc cgtttgcatg
1021 gggcaaaaat gccgaagatg cggtgcataa cgccatcgtg ctggaagagg tcgcttatat
1081 ggggatattc tgccgtcagt tagcgccgca gttaccggat atgcagcaaa cgctgctgga
1141 taaacactat ctgcgtaagc atggcgcgaa ggcatattac gggcagtaat gactgtataa
1201 aaccacagcc aatcaaacga aaccaggcta tactcaagcc tggttttttg atggattttc
1261 agcgtggcgc aggcaggttt tatcttaacc cgacactggc gggacacccc gcaagggaca
1321 gaagtctcct tctggctggc gacggacaac gggccgttgc aggttacgct tgcaccgcaa
1381 gagtccgtgg cgtttattcc cgccgatcag gttccccgcg ctcagcatat tttgcagggt
1441 gaacaaggct ttcgcctgac accgctggcg ttaaaggatt ttcaccgcca gccggtgtat
1501 ggcctttact gtcgcgccca tcgccaattg atgaattacg aaaagcgcct gcgtgaaggt
1561 ggcgttaccg tctacgaggc cgatgtgcgt ccgccagaac gctatctgat ggagcggttt
1621 atcacctcac cggtgtgggt cgagggtgat atgcacaatg gcactatcgt taatgcccgt
1681 ctgaaaccgc atcccgacta tcgtccgccg ctcaagtggg tttctataga tattgaaacc
1741 acccgccacg gtgagctgta ctgcatcggc ctggaagcgt gcgggcagcg catcgtttat
1801 atgctggggc cggagaatgg cgacgcctcc tcgcttgatt tcgaactgga atacgtcgcc
1861 agccgcccgc agttgctgga aaaactcaac gcctggtttg ccaactacga tcctgatgtg
1921 atcatcggtt ggaacgtggt gcagttcgat ctgcgaatgc tgcaaaaaca tgccgagcgt
1981 taccgtcttc cgctgcgtct tgggcgcgat aatagcgagc tggagtggcg cgagcacggc
2041 tttaaaaacg gcgtcttttt tgcccaggct aaaggtcggc taattatcga cggtatcgag
2101 gcgctgaaat ccgcgttctg gaatttctct tcattctcgc tggaaactgt cgctcaggag
2161 ctattaggcg aaggaaaatc tatcgataac ccgtgggatc gaatggacga aattgaccgc
2221 cgtttcgccg aagataaacc tgcgctggca acttataacc tgaaagattg cgagctggtg
2281 acgcagatct tccacaaaac tgaaatcatg ccatttttac tcgaacgggc aacggtgaac
2341 ggcctgccgg tggaccgaca cggcggttcg gtggcggcat ttggtcatct ctattttccg
2401 cgaatgcatc gcgctggtta tgtcgcgcct aatctcggcg aagtgccgcc gcacgccagc
2461 cctggcggct acgtgatgga ttcacggcca gggctttatg attcagtgct ggtgctggac
2521 tataaaagcc tgtacccgtc gatcatccgc acctttctga ttgatcccgt cgggctggtg
2581 gaaggcatgg cgcagcctga tccagagcac agtaccgaag gttttctcga tgcctggttc
2641 tcgcgagaaa aacattgcct gccggagatt gtgactaaca tctggcacgg gcgcgatgaa
2701 gccaaacgcc agggtaacaa accgctgtcg caggcgctga aaatcatcat gaatgccttt
2761 tatggcgtgc tcggcaccac cgcctgccgc ttcttcgatc cgcggctggc atcgtcgatc
2821 accatgcgtg gtcatcagat catgcggcaa accaaagcgt tgattgaagc acagggctac
2881 gacgttatct acggcgatac cgactcaacg tttgtctggc tgaaaggcgc acattcggaa
2941 gaagaagcgg cgaaaatcgg tcgtgcactg gtgcagcacg ttaacgcctg gtgggcggaa
3001 acgctgcaaa aacaacggct gaccagcgca ttagaactgg agtatgaaac ccatttctgc
3061 cgttttctga tgccaaccat tcgcggagcc gataccggca gtaaaaagcg ttatgccgga
3121 ctgattcagg agggcgacaa gcagcggatg gtgtttaaag ggctggaaac cgtgcgcacc
3181 gactggacgc cgctggccca gcagtttcag caggagctat acctgcgcat cttccgcaac
3241 gagccatatc aggaatatgt acgcgaaacc atcgacaaac tgatggcggg tgaactggat
3301 gcgcgactgg tttaccgtaa acgccttcgc cgtccgctga gcgagtatca gcgtaatgtg
3361 ccgcctcatg tacgcgccgc tcgccttgcc gatgaagaaa accaaaagcg tggtcgcccc
3421 ttgcaatatc agaatcgcgg caccattaag tacgtatgga ccaccacagg cccggagccg
3481 cctggactac caacgttcac cactggatta cgaacactat ctgacccgcc agctacaacc
3541 cgtggcggag ggaatactcc cttttattga ggataatttt gctacactta tgaccgggca
3601 acttgggcta ttttgagcaa aaaaaagagt tcgccagata ccattttgat gcgtgacgaa
3661 tgctttgcca tccagtacca tagcgccctt tccattcctg gacctgaata acaccactac
3721 ctcataagca cggtagcggg tggttattgc ctgcaattaa agatatagag ccgaacacat
3781 atgcctttta cacttggtca acgctggatc agcgatacag aaagcgaatt gggacttgga
3841 accgttgtcg cggtggatgc gcgaactgtc actttacttt tcccatctac tggtgaaaac
3901 cgtctgtacg cacgcagtga ttcccccgtg acccgcgtga tgttcaaccc tggtgatacc
3961 attaccagcc atgacggctg gcagatgcaa gtcgaagaag taaaagaaga aaatggcttg
4021 ctgacctata tcggtactcg cctggatact gaagaggtcc ggcgtagccc tgcgtgaagt
4081 tttccttgat agcaaactgg tgttcagcaa accgcaggca ccgtctgttt gccgggcaga
4141 ttgaccgtat ggaccgcttt gcgctgcgtt atcgcgcgcg taaatattcc agcgaacagt
4201 tccgtatgcc gtacagcggc ctgcgcggtc agcgtaccag cctgatccgc atcagctcaa
4261 catcgctcat gatgttggtc gccgccacgc gccgcgcgtc ctgctggctg acgaagtggg
4321 tttagggaaa accattgaag ccgggatgat cctgcatcag caactgctct ctggcgctgc
4381 tgaacgtgtg ctaattatcg tcccggaaac cttacagcat cagtggctgg tagaaatgct
4441 gcgccgtttc aacctgcgct ttgcgctatt tgatgatgag cgttatgccg aagctcagca
4501 cgatgcttac aacccgtttg acaccgtgaa gcggcgcacg aaaaacgcga aagcgtttca
4561 cgataaatgc gaaaacttta gctttcgcgc ttcaaatgaa acagatgtat taattactgc
4621 tttttattca ttacatgggg atccccgggt accgagctcg aattcc
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