GenBank-Updates@genbank.bio.net (09/02/90)
LOCUS ECOUNC 7881 bp ds-DNA BCT 02-SEP-1990
DEFINITION E.coli atp (unc) operon containing nine genes coding for ATP
synthase subunits.
ACCESSION J01594 J01595 V00313 K02181 V00264 X00771 M25634
KEYWORDS ATP operon; ATP synthetase; ATPase;
adenine triphosphate synthetase; pap operon; synthetase;
unc operon.
SOURCE Escherichia coli DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 3169 to 4701)
AUTHORS Gay,N.J. and Walker,J.E.
TITLE The atp operon: Nucleotide sequence of the region encoding the
alpha-subunit of Escherichia coli ATP-synthase
JOURNAL Nucleic Acids Res. 9, 2187-2194 (1981)
STANDARD simple staff_review
REFERENCE 2 (bases 812 to 3311)
AUTHORS Nielsen,J., Hansen,F.G., Hoppe,J., Friedl,P. and Von Meyenburg,K.
TITLE The nucleotide sequence of the atp genes coding for the F-0
subunits a, b, c and the F-1 subunit delta of the membrane bound
ATP synthase of Escherichia coli
JOURNAL Mol. Gen. Genet. 184, 33-39 (1981)
STANDARD simple staff_review
REFERENCE 3 (bases 389 to 3173)
AUTHORS Gay,N.J. and Walker,J.E.
TITLE The atp operon: Nucleotide sequence of the promoter and the genes
for the membrane proteins, and the delta subunit of Escherichia
coli ATP-synthase
JOURNAL Nucleic Acids Res. 9, 3919-3926 (1981)
STANDARD simple staff_review
REFERENCE 4 (bases 3160 to 5694)
AUTHORS Kanazawa,H., Kayano,T., Mabuchi,K. and Futai,M.
TITLE Nucleotide sequence of the genes coding for alpha, beta and gamma
subunits of the proton-translocating ATPase of Escherichia coli
JOURNAL Biochem. Biophys. Res. Commun. 103, 604-612 (1981)
STANDARD simple staff_review
REFERENCE 5 (bases 506 to 2622)
AUTHORS Kanazawa,H., Mabuchi,K., Kayano,T., Noumi,T., Sekiya,T. and
Futai,M.
TITLE Nucleotide sequence of the genes for F-0 components of the
proton-translocating ATPase from Escherichia coli: Prediction of
the primary structure of F-0 subunits
JOURNAL Biochem. Biophys. Res. Commun. 103, 613-620 (1981)
STANDARD simple staff_review
REFERENCE 6 (bases 5641 to 7540)
AUTHORS Kanazawa,H., Kayano,T., Kiyasu,T. and Futai,M.
TITLE Nucleotide sequence of the genes for beta and epsilon subunits of
proton-translocating ATPase from Escherichia coli
JOURNAL Biochem. Biophys. Res. Commun. 105, 1257-1264 (1982)
STANDARD simple staff_review
REFERENCE 7 (bases 1 to 817)
AUTHORS Kanazawa,H., Mabuchi,K. and Futai,M.
TITLE Nucleotide sequence of the promoter region of the gene cluster for
proton-translocating ATPase from Escherichia coli and
identification of the active promoter
JOURNAL Biochem. Biophys. Res. Commun. 107, 568-575 (1982)
STANDARD simple staff_review
REFERENCE 8 (bases 565 to 957)
AUTHORS Gay,N.J.
TITLE Construction and characterization of an Escherichia coli strain
with an unc1 mutation
JOURNAL J. Bacteriol. 158, 820-825 (1984)
STANDARD simple staff_review
REFERENCE 9 (bases 2129 to 2599)
AUTHORS Jans,D.A., Fimmel,A.L., Hatch,L., Gibson,F. and Cox,G.B.
TITLE An additional acidic residue in the membrane portion of the
b-subunit of the energy-transducing adenosine triphosphatase of
Escherichia coli affects both assembly and function
JOURNAL Biochem. J. 221, 43-51 (1984)
STANDARD simple staff_review
REFERENCE 10 (bases 1 to 7881; revises other sequences)
AUTHORS Walker,J.E., Saraste,M. and Gay,N.J.
TITLE Nucleotide sequence, regulation and structure of ATP-synthase
JOURNAL Biochim. Biophys. Acta 768, 164-200 (1984)
STANDARD simple staff_entry
COMMENT The ATP synthase operon consists of nine genes coding for nine
structural proteins of ATPase. It is not known through these
references whether these cistrons are transcribed as one or several
transcripts (the subunits are not found in stoichiometric amounts).
Cistrons 2, 3 and 4 encode the so-called F-0 components which are
membrane bound and said to serve the purpose of proton channeling;
the 5th through 9th cistrons encode the F-1 components which are
the peripheral membrane portion responsible for actual catalysis of
ATP synthesis. The cistronic order (counterclockwise on the E.coli
chromosome) and the alternative designations for these functions
are summarized:
atp-1 .... UncI polypeptide ..................... uncI.
atp-2 .... a subunit ........ papD .... atpB .... uncB.
atp-3 .... c subunit ........ papH .... atpE .... uncE.
atp-4 .... b subunit ........ papF .... atpF .... uncF.
atp-5 .... delta subunit .... papE .... atpH ....
atp-6 .... alpha subunit .... papA .... atpA .... uncA.
atp-7 .... gamma subunit .... papC .... atpG ....
atp-8 .... beta subunit ..... papB .... atpD .... uncD.
atp-9 .... epsilon subunit .. papG .... atpC ....
[5] is revised by [7]; due to the large number of revisions, they
have not been presented as individual revisions in the FEATURES
Table.
FEATURES from to/span description
pept 623 1015 ATP synthase UncI polypeptide (atp-1; gtg
start codon) /nomgen="uncI"
pept 1024 1839 ATP synthase a subunit (atp-2) /nomgen="uncB"
pept 1886 2125 ATP synthase c subunit (atp-3) /nomgen="uncE"
pept 2187 2657 ATP synthase b subunit (atp-4; gtg start
codon) /nomgen="uncF"
pept 2672 3205 ATP synthase delta subunit (atp-5)
/nomgen="uncH"
pept 3218 4759 ATP synthase alpha subunit (atp-6)
/nomgen="uncA"
pept 4810 5673 ATP synthase gamma subunit (atp-7)
/nomgen="uncG"
pept 5700 7082 ATP synthase beta subunit (atp-8)
/nomgen="uncD"
pept 7103 7522 ATP synthase epsilon subunit (atp-9)
/nomgen="uncC"
pept 7875 > 7880 glucosaminephosphate isomerase (glmS)
signal 513 549 atp operon putative promoter [3]
signal 7531 7576 transcription termination signal [10]
mut 675 870 tt...bp...aa in wt; ta in mutant SD42
BASE COUNT 1924 a 1926 c 2098 g 1933 t
ORIGIN 61 bp upstream of HpaI site [7].
1 aaagcaaata aaatttaatt tttatcaaaa aaatcataaa aaattgaccg gttagactgt
61 taacaacaac caggttttct actgatataa ctggttacat ttaacgccac gttcactctt
121 ttgcatcaac aagataacgt ggcttttttt ggtaagcaga aaataagtca ttagtgaaaa
181 tatcagtctg ctaaaaatcg gcgctaagaa ccatcattgg ctgttaaaac attattaaaa
241 atgtcaatgg gtggtttttg ttgtgtaaat gtcatttatt aaaacagtat ctgtttttag
301 actgaaatat cataaacttg caaaggcatc atttgccaag taaataaata tgctgtgcgc
361 gaacatgcgc aatatgtgat ctgaagcacg ctttatcacc agtgtttacg cgttatttac
421 agtttttcat gatcgaacag ggttagcaga aaagtcgcaa ttgtatgcac tggaaaaata
481 tttaaacatt tattcacctt ttggctactt attgtttgaa atcacggggg cgcaccgtat
541 aatttgaccg ctttttgatg cttgactcta agccttaaag aaagttttat acgacacgcg
601 gcatacctcg aagggagcag gagtgaaaaa cgtgatgtct gtgtcgctcg tgagtcgaaa
661 cgttgctcgg aagcttctgc tcgttcagtt actggtggtg atagcaagtg gattgctgtt
721 cagcctcaaa gaccccttct ggggcgtctc tgcaataagc gggggcctgg cagtctttct
781 gcctaacgtt ttgtttatga tatttgcctg gcgtcaccag gcgcatacac cagcgaaagg
841 ccgggtggcc tggacattcg catttggcga agctttcaaa gttctggcga tgttggtgtt
901 actggtggtg gcgttggcgg ttttaaaggc ggtattcttg ccgctgatcg ttacgtgggt
961 tttggtgctg gtggttcaga tactggcacc ggctgtaatt aacaacaaag ggtaaaaggc
1021 atcatggctt cagaaaatat gacgccgcag gattacatag gacaccacct gaataacctt
1081 cagctggacc tgcgtacatt ctcgctggtg gatccacaaa accccccagc caccttctgg
1141 acaatcaata ttgactccat gttcttctcg gtggtgctgg gtctgttgtt cctggtttta
1201 ttccgtagcg tagccaaaaa ggcgaccagc ggtgtgccag gtaagtttca gaccgcgatt
1261 gagctggtga tcggctttgt taatggtagc gtgaaagaca tgtaccatgg caaaagcaag
1321 ctgattgctc cgctggccct gacgatcttc gtctgggtat tcctgatgaa cctgatggat
1381 ttactgccta tcgacctgct gccgtacatt gctgaacatg tactgggtct gcctgcactg
1441 cgtgtggttc cgtctgcgga cgtgaacgta acgctgtcta tggcactggg cgtatttatc
1501 ctgattctgt tctacagcat caaaatgaaa ggcatcggcg gcttcacgaa agagttgacg
1561 ctgcagccgt tcaatcactg ggcgttcatt cctgtcaact taatccttga aggggtaagc
1621 ctgctgtcca aaccagtttc actcggtttg cgactgttcg gtaacatgta tgccggtgag
1681 ctgattttca ttctgattgc tggtctgttg ccgtggtggt cacagtggat cctgaatgtg
1741 ccgtgggcca ttttccacat cctgatcatt acgctgcaag ccttcatctt catggttctg
1801 acgatcgtct atctgtcgat ggcgtctgaa gaacattaat ttaccaacac tactacgttt
1861 taactgaaac aaactggaga ctgtcatgga aaacctgaat atggatctgc tgtacatggc
1921 tgccgctgtg atgatgggtc tggcggcaat cggtgctgcg atcggtatcg gcatcctcgg
1981 gggtaaattc ctggaaggcg cagcgcgtca acctgatctg attcctctgc tgcgtactca
2041 gttctttatc gttatgggtc tggtggatgc tatcccgatg atcgctgtag gtctgggtct
2101 gtacgtgatg ttcgctgtcg cgtagtaagc gttgctttta tttaaagagc aatatcagaa
2161 cgttaactaa atagaggcat tgtgctgtga atcttaacgc aacaatcctc ggccaggcca
2221 tcgcgtttgt cctgttcgtt ctgttctgca tgaagtacgt atggccgcca ttaatggcag
2281 ccatcgaaaa acgtcaaaaa gaaattgctg acggccttgc ttccgcagaa cgagcacata
2341 aggaccttga ccttgcaaag gccagcgcga ccgaccagct gaaaaaagcg aaagcggaag
2401 cccaggtaat catcgagcag gcgaacaaac gccgctcgca gattctggac gaagcgaaag
2461 ctgaggcaga acaggaacgt actaaaatcg tggcccaggc gcaggcggaa attgaagccg
2521 agcgtaaacg tgcccgtgaa gagctgcgta agcaagttgc tatcctggct gttgctggcg
2581 ccgagaagat catcgaacgt tccgtggatg aagctgctaa cagcgacatc gtggataaac
2641 ttgtcgctga actgtaagga gggaggggct gatgtctgaa tttattacgg tagctcgccc
2701 ctacgccaaa gcagcttttg actttgccgt cgaacaccaa agtgtagaac gctggcagga
2761 catgctggcg tttgccgccg aggtaaccaa aaacgaacaa atggcagagc ttctctctgg
2821 cgcgcttgcg ccagaaacgc tcgccgagtc gtttatcgca gtttgtggtg agcaactgga
2881 cgaaaacggt cagaacctga ttcgggttat ggctgaaaat ggtcgtctta acgcgctccc
2941 ggatgttctg gagcagttta ttcacctgcg tgccgtgagt gaggctaccg ctgaggtaga
3001 cgtcatttcc gctgccgcac tgagtgaaca acagctcgcg aaaatttctg ctgcgatgga
3061 aaaacgtctg tcacgcaaag ttaagctgaa ttgcaaaatc gataagtctg taatggcagg
3121 cgttatcatc cgagcgggtg atatggtcat tgatggcagc gtacgcggtc gtcttgagcg
3181 ccttgcagac gtcttgcagt cttaagggga ctggagcatg caactgaatt ccaccgaaat
3241 cagcgaactg atcaagcagc gcattgctca gttcaatgtt gtgagtgaag ctcacaacga
3301 aggtactatt gtttctgtaa gtgacggtgt tatccgcatt cacggcctgg ccgattgtat
3361 gcagggtgaa atgatctccc tgccgggtaa ccgttacgct atcgcactga acctcgagcg
3421 cgactctgta ggtgcggttg ttatgggtcc gtacgctgac cttgccgaag gcatgaaagt
3481 taagtgtact ggccgtatcc tggaagttcc ggttggccgt ggcctgctgg gccgtgtggt
3541 taacactctg ggtgcaccaa tcgacggtaa aggtccgctg gatcacgacg gcttctctgc
3601 tgtagaagca atcgctccgg gcgttatcga acgtcagtcc gtagatcagc cggtacagac
3661 cggttataaa gccgttgact ccatgatccc aatcggtcgt ggtcagcgtg aattgatcat
3721 cggtgaccgt cagacaggta aaaccgcact ggctatcgat gccatcatca accagcgcga
3781 ttccggtatc aaatgtatct atgtcgctat cggccagaaa gcgtccacca tttctaacgt
3841 ggtacgtaaa ctggaagagc acggcgcact ggctaacacc atcgttgtgg tagcaaccgc
3901 gtctgaatcc gctgcactgc aatacctggc acgtatgccg gttgcgctaa tgggcgaata
3961 cttccgtgac cgcggtgaag atgcgctgat catttacgat gacctgtcta aacaggctgt
4021 tgcttaccgt cagatctccc tgctgctccg tcgtccgcca ggacgtgaag cattcccggg
4081 cgacgttttc tacctccact ctcgtctgct ggagcgtgct gcacgtgtta acgccgaata
4141 cgttgaagcc ttcaccaaag gtgaagtgaa agggaaaacc ggttctctga ccgcactgcc
4201 gattatcgaa actcaggcgg gtgacgtttc tgcgttcgtt ccgaccaacg taatctccat
4261 taccgatggt cagatcttcc tggaaaccaa cctgttcaac gccggtattc gtcctgcggt
4321 taacccgggt atttccgtat cccgtgttgg tggtgcagca cagaccaaga tcatgaaaaa
4381 actgtccggt ggtatccgta ccgctctggc acagtatcgt gaactggcag cgttctctca
4441 gtttgcatcc gaccttgacg atgcaacacg taaccagctt gaccacggtc agaaagtgac
4501 cgaactgctg aaacagaaac agtatgcgcc gatgtccgtt gcgcagcagt ctctggttct
4561 gttcgcagca gaacgtggtt acctggcgga tgttgaactg tcgaaaattg gcagcttcga
4621 agccgctctg ctggcttacg tcgaccgtga tcacgctccg ttgatgcaag agatcaacca
4681 gaccggtggc tacaacgacg aaatcgaagg caagctgaaa ggcatcctcg attccttcaa
4741 agcaacccaa tcctggtaac gtctggcggc ttgccttagg gcaggccgca aggcattgag
4801 gagaagctca tggccggcgc aaaagacata cgtagtaaga tcgcaagcgt ccagaacacg
4861 caaaagatca ctaaagcgat ggagatggtc gccgcttcca aaatgcgtaa atcgcaggat
4921 cgcatggcgg ccagccgtcc ttatgcagaa accatgcgca aagtgattgg tcaccttgca
4981 cacggtaatc tggaatataa gcacccttac ctggaagacc gcgacgttaa acgcgtgggc
5041 tacctggtgg tgtcgaccga ccgtggtttg tgcggtggtt tgaacattaa cctgttcaaa
5101 aaactgctgg cggaaatgaa gacctggacc gacaaaggcg ttcaatgcga cctcgcaatg
5161 atcggctcga aaggcgtgtc gttcttcaac tccgtgggcg gcaatgttgt tgcccaggtc
5221 accggcatgg gggataaccc ttccctgtcc gaactgatcg gtccggtaaa agtgatgttg
5281 caggcctacg acgaaggccg tctggacaag ctttacattg tcagcaacaa atttattaac
5341 accatgtctc aggttccgac catcagccag ctgctgccgt taccggcatc agatgatgat
5401 gatctgaaac ataaatcctg ggattacctg tacgaacccg atccgaaggc gttgctggat
5461 accctgctgc gtcgttatgt cgaatctcag gtttatcagg gcgtggttga aaacctggcc
5521 agcgagcagg ccgcccgtat ggtggcgatg aaagccgcga ccgacaatgg cggcagcctg
5581 attaaagagc tgcagttggt atacaacaaa gctcgtcagg ccagcattac tcaggaactc
5641 accgagatcg tctcgggggc cgccgcggtt taaacaggtt atttcgtaga ggatttaaga
5701 tggctactgg aaagattgtc caggtaatcg gcgccgtagt tgacgtcgaa ttccctcagg
5761 atgccgtacc gcgcgtgtac gatgctcttg aggtgcaaaa tggtaatgag cgtctggtgc
5821 tggaagttca gcagcagctc ggcggcggta tcgtacgtac catcgcaatg ggttcctccg
5881 acggtctgcg tcgcggtctg gatgtaaaag acctcgaaca cccgattgaa gtcccggtag
5941 gtaaagcgac tctgggccgt atcatgaacg tactgggtga accggtcgac atgaaaggcg
6001 agatcggtga agaagagcgt tgggcgattc accgcgcagc accttcctac gaagagctgt
6061 caaactctca ggaactgctg gaaaccggta tcaaagttat cgacctgatg tgtccgttcg
6121 ctaagggcgg taaagttggt ctgttcggtg gtgcgggtgt aggtaaaacc gtaaacatga
6181 tggagctcat tcgtaacatc gcgatcgagc actccggtta ctctgtgttt gcgggcgtag
6241 gtgaacgtac tcgtgagggt aacgacttct accacgaaat gaccgactcc aacgttatcg
6301 acaaagtatc cctggtgtat ggccagatga acgagccgcc gggaaaccgt ctgcgcgttg
6361 ctctgaccgg tctgaccatg gctgagaaat tccgtgacga aggtcgtgac gttctgctgt
6421 tcgttgacaa catctatcgt tacaccctgg ccggtacgga agtatccgca ctgctgggcc
6481 gtatgccttc agcggtaggt tatcagccga ccctggcgga agagatgggc gttctgcagg
6541 aacgtatcac ctccaccaaa actggttcta tcacctccgt acaggcagta tacgtacctg
6601 cggatgactt gactgacccg tctccggcaa ccacctttgc gcaccttgac gcaaccgtgg
6661 tactgagccg tcagatcgcg tctctgggta tctacccggc cgttgacccg ctggactcca
6721 ccagccgtca gctggacccg ctggtggttg gtcaggaaca ctacgacacc gcgcgtggcg
6781 ttcagtccat cctgcaacgt tatcaggaac tgaaagacat catcgccatc ctgggtatgg
6841 atgaactgtc tgaagaagac aaactggtgg tagcgcgtgc tcgtaagatc cagcgcttcc
6901 tgtcccagcc gttcttcgtg gcagaagtat tcaccggttc tccgggtaaa tacgtctccc
6961 tgaaagacac catccgtggc tttaaaggca tcatggaagg cgaatacgat cacctgccgg
7021 agcaggcgtt ctacatggtc ggttccatcg aagaagctgt ggaaaaagcc aaaaaacttt
7081 aacgccttaa tcggagggtg atatggcaat gacttaccac ctggacgtcg tcagcgcaga
7141 gcaacaaatg ttctctggtc tggtcgagaa aatccaggta acgggtagcg aaggtgaact
7201 ggggatctac cctggccacg caccgctgct caccgccatt aagcctggta tgattcgcat
7261 cgtgaaacag cacggtcacg aagagtttat ctatctgtct ggcggcattc ttgaagtgca
7321 gcctggcaac gtgaccgttc tggccgacac cgcaattcgc ggccaggatc tcgacgaagc
7381 gcgagccatg gaagcgaaac gtaaggctga agagcacatt agcagctctc acggcgacgt
7441 agattacgct caggcgtctg cggaactggc caaagcgatc gcgcagctgc gcgttatcga
7501 gttgaccaaa aaagcgatgt aacaccggct tgaaaagcac aaaagccagt ctggaaacag
7561 gctggctttt ttttgcgcgt gtgacccgtc ctgaatagcg ttcacataga tcctgctgat
7621 ataaaacccc cctgttttcc tgtttattca ttgatcgaaa taagagcaaa aacatccacc
7681 tgacgcttaa attaaggtac tgccttaatt ttctgcagac aaaaggcgtg acgatggtcg
7741 aaaatggcgc tttcgtcagc ggggataatc cgttattgaa caatttatcc tctgtccatt
7801 tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt tctcaaatta
7861 cagtcaggac gcgtatgttg a
//