GenBank-Updates@genbank.bio.net (09/02/90)
LOCUS NG2MET 1810 bp ds-DNA BCT 02-SEP-1990
DEFINITION Plasmid pNG2 (from C.diphtheriae) methylase gene, complete cds.
ACCESSION M36726
KEYWORDS erythromycin-resistance; methylase.
SOURCE Plasmid pNG2 (from Corynebacterium diphtheria) DNA.
ORGANISM Plasmid pNG2
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 1810)
AUTHORS Serwold-Davis,T.M. and Groman,N.B.
TITLE Identification of a methylase gene for erythromycin resistance
within the sequence of a spontaneously deleting fragment of
Corynebacterium diphtheriae plasmid pNG2
JOURNAL FEMS Microbiol. Lett. 56, 7-14 (1988)
STANDARD simple staff_entry
FEATURES from to/span description
pept 150 215 erythromycin resistance protein leader peptide
pept 296 1150 erythromycin resistance protein (ermCd)
signal 1148 1218 transcription termination signal
BASE COUNT 439 a 541 c 448 g 382 t
ORIGIN
1 aagcttgggc taccgcacgc cagctgaagt ggaaaccgaa ttttgggacc agcacccgcc
61 gcaagcaata atagaaatca aggcaaacgc ctaggaacaa aacccggggc acttcacacc
121 cgcgaattta aatggatata attagaccca tgttgatttc aggtaccgct ttcttgcggt
181 tgcgcaccaa ccgtaaggcg tttcctaccc cgtaggttaa aaaccgctcc ggtcctttag
241 gccgggcggc tatttgtcat gcccggctcc ctttcaaatc caaggagcac acctgatgtc
301 tgcatacgga cagggccgtc acgagcatgg ccaaaatttt ctcaccaacc acaagatcat
361 caactccatc atcgaccttg tgaaacaaac ctccggcccc atcattgaga tcggaccagg
421 aagcggtgcc ctcactcacc cgatggccca cttggggagg gcgataacgg cagttgaagt
481 ggacgcaaaa ctagctgcca aaatcacaca agaaacctcc tcggcggcgg tcgaagtggt
541 ccatgatgat ttccttaact tccggttacc cgccactccc tgcgtcattg tgggaaacat
601 tccctttcac ctcaccactg ccattcttcg aaagttgctg catgcgccag catggactga
661 cgctgtactc ctcatgcagt gggaagtcgc tcgccgccgg gccggggtag gcgcaacgac
721 gatgatgacg gctcagtggt ccccatggtt cacatttcac ctgggttctc gggtaccaag
781 gtctgctttc cggccacagc caaacgttga cggggggatc ttagtgatcc gccgggtggg
841 tgacccgaag attccgatag agcagcgcaa agcctttcag gcgatggtgc acaccgtttt
901 cactgcccgg ggacgcggga taggggaaat tctccgaagg cagggttgtt ttcatcacgt
961 tcagaaacat aatcatggtt gcgctcgcga ggaatcgacc ccgcgaccct acctcccaga
1021 ttgctacacc aacgactgga tcgatctctt ccaggtgact ggttcctctc tacctcacca
1081 tcgacccatt tcaccatcgg gaagtagtca acgacctcct caacggaaaa accgaagccg
1141 gcggcgttaa tccccaccaa aaccgaaatc caccagtagt actaaaaggg ctcttccggt
1201 tttagagtgc attgattagt tcgccggggg ttcgggcgtg gcacaacata ttggggtcct
1261 tggctttgta ggaaaaaggt atctaatcca tccgtacaaa actaaggacc tactgtgcag
1321 ccctaacgga aatgtcatcg tcgataccat ctgccgcacc gcaaaactag gaactactat
1381 caccggtgcc acagaaaacg gtgacgtcac tgttattgaa gccgaacccg tcgagccgat
1441 caatgaatgc cctacctgcg ggcagcctgg agtattccgc gaccacgtca tccgcagcct
1501 ggtcgatctg cccatcgtcg gccacccaac aacttcatgt gcgccttcca cgctaccggt
1561 gcaccaacaa gcgttgcttg cagaagatct tccgtgctgg ccttgcctgc gcgcccgaca
1621 attcaaaaga ccacggacca ggtgacccac tggatcctgc aacgactctc ctaaatcgga
1681 tgagcgttgc cgctgcaccc aagtcattag gcctggggtg ggatatgaag tgtcccaggt
1741 tttgttccgt ttgatgaaac ccgtttttga aacaacgtac ggagcacaac gcacgccgat
1801 gccaaagctt
//GenBank-Updates@genbank.bio.net (09/05/90)
LOCUS NG2MET 1810 bp ds-DNA BCT 05-SEP-1990
DEFINITION Plasmid pNG2 (from C.diphtheriae) methylase gene, complete cds.
ACCESSION M36726
KEYWORDS erythromycin-resistance; methylase.
SOURCE Plasmid pNG2 (from Corynebacterium diphtheria) DNA.
ORGANISM Plasmid pNG2
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 1810)
AUTHORS Serwold-Davis,T.M. and Groman,N.B.
TITLE Identification of a methylase gene for erythromycin resistance
within the sequence of a spontaneously deleting fragment of
Corynebacterium diphtheriae plasmid pNG2
JOURNAL FEMS Microbiol. Lett. 56, 7-14 (1988)
STANDARD simple staff_entry
FEATURES from to/span description
pept 150 215 erythromycin resistance protein (ermCd) leader
peptide
pept 296 1150 erythromycin resistance protein (ermCd)
signal 1148 1214 transcription termination signal
BASE COUNT 439 a 541 c 448 g 382 t
ORIGIN
1 aagcttgggc taccgcacgc cagctgaagt ggaaaccgaa ttttgggacc agcacccgcc
61 gcaagcaata atagaaatca aggcaaacgc ctaggaacaa aacccggggc acttcacacc
121 cgcgaattta aatggatata attagaccca tgttgatttc aggtaccgct ttcttgcggt
181 tgcgcaccaa ccgtaaggcg tttcctaccc cgtaggttaa aaaccgctcc ggtcctttag
241 gccgggcggc tatttgtcat gcccggctcc ctttcaaatc caaggagcac acctgatgtc
301 tgcatacgga cagggccgtc acgagcatgg ccaaaatttt ctcaccaacc acaagatcat
361 caactccatc atcgaccttg tgaaacaaac ctccggcccc atcattgaga tcggaccagg
421 aagcggtgcc ctcactcacc cgatggccca cttggggagg gcgataacgg cagttgaagt
481 ggacgcaaaa ctagctgcca aaatcacaca agaaacctcc tcggcggcgg tcgaagtggt
541 ccatgatgat ttccttaact tccggttacc cgccactccc tgcgtcattg tgggaaacat
601 tccctttcac ctcaccactg ccattcttcg aaagttgctg catgcgccag catggactga
661 cgctgtactc ctcatgcagt gggaagtcgc tcgccgccgg gccggggtag gcgcaacgac
721 gatgatgacg gctcagtggt ccccatggtt cacatttcac ctgggttctc gggtaccaag
781 gtctgctttc cggccacagc caaacgttga cggggggatc ttagtgatcc gccgggtggg
841 tgacccgaag attccgatag agcagcgcaa agcctttcag gcgatggtgc acaccgtttt
901 cactgcccgg ggacgcggga taggggaaat tctccgaagg cagggttgtt ttcatcacgt
961 tcagaaacat aatcatggtt gcgctcgcga ggaatcgacc ccgcgaccct acctcccaga
1021 ttgctacacc aacgactgga tcgatctctt ccaggtgact ggttcctctc tacctcacca
1081 tcgacccatt tcaccatcgg gaagtagtca acgacctcct caacggaaaa accgaagccg
1141 gcggcgttaa tccccaccaa aaccgaaatc caccagtagt actaaaaggg ctcttccggt
1201 tttagagtgc attgattagt tcgccggggg ttcgggcgtg gcacaacata ttggggtcct
1261 tggctttgta ggaaaaaggt atctaatcca tccgtacaaa actaaggacc tactgtgcag
1321 ccctaacgga aatgtcatcg tcgataccat ctgccgcacc gcaaaactag gaactactat
1381 caccggtgcc acagaaaacg gtgacgtcac tgttattgaa gccgaacccg tcgagccgat
1441 caatgaatgc cctacctgcg ggcagcctgg agtattccgc gaccacgtca tccgcagcct
1501 ggtcgatctg cccatcgtcg gccacccaac aacttcatgt gcgccttcca cgctaccggt
1561 gcaccaacaa gcgttgcttg cagaagatct tccgtgctgg ccttgcctgc gcgcccgaca
1621 attcaaaaga ccacggacca ggtgacccac tggatcctgc aacgactctc ctaaatcgga
1681 tgagcgttgc cgctgcaccc aagtcattag gcctggggtg ggatatgaag tgtcccaggt
1741 tttgttccgt ttgatgaaac ccgtttttga aacaacgtac ggagcacaac gcacgccgat
1801 gccaaagctt
//