GenBank-Updates@genbank.bio.net (09/05/90)
LOCUS STALACR 2369 bp ds-DNA BCT 05-SEP-1990
DEFINITION S.aureus lac repressor (lacR) gene, complete cds, and lacA
repressor, 5' end.
ACCESSION M32103
KEYWORDS lac repressor.
SOURCE S.aureus (strain 8325-4) DNA.
ORGANISM Staphylococcus aureus
Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
Micrococcaceae.
REFERENCE 1 (bases 1 to 2369)
AUTHORS Oskouian,B. and Stewart,G.C.
TITLE Repression and catabolite repression of the lactose operon of
Staphylococcus aureus
JOURNAL J. Bacteriol. 172, 3804-3812 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence [1] kindly submitted by
G.C.Stewart, 14-FEB-1990. Author confirmed ttg start codon of the
lacR coding region by phone 09-APR-1990.
FEATURES from to/span description
pept 1057 1812 lacR repressor (ttg start codon)
pept 2181 > 2369 lacA repressor
binding 1039 1046 lacR ribosome binding site
binding 2166 2172 lacA ribosome binding site
rpt 1992 2001 direct repeat
rpt 2046 2055 direct repeat
rpt 2011 2022 inverted repeat
rpt 2050 2061 inverted repeat
signal 886 857 (c) ORF-26 promoter
signal 2042 2070 lacR promoter
BASE COUNT 866 a 364 c 400 g 739 t
ORIGIN Map position 8b of the 18 division map.
1 ctccaaattc caaaacagca actccaaaat taaaagcatt tccctaccat tcgggaaatg
61 ctttttacat actgattact ctgtcattaa tgattttaca acggaaacca tgtcgtcatg
121 tatgaccaaa gtagcgtcgc tatcataagg tgttcgatct ttattgataa taattaaatt
181 gtcgccttta aaatgtgata ttaatcctgc ggcaggttgt acaacgagtg atgaacctag
241 tacaacaagg gtgtcagcat gttcaatttt atttaatgcc cttatgatgg taggttgatc
301 taacatttca ccgtataata cgatgtccgg tcgaatggca ccaccacaat tatcacaatg
361 tttcaaagtt ctatcaataa catctgactt cgtataagat ttatgacata cattacaata
421 aaaacgattt aacgtgccat gtaattcatc aacatgttga cttccagcgt ctgagtgcaa
481 accatcgata ttttgcgtga tgacacctaa agattgttga ttacgttcta attttgcaat
541 ccaatcatga acgatattgg gcatcgtatc gacaaatagt aagcgcttat ggcagaaatt
601 gataaaacct tcaggatcat cttctaaata atcacggctt aacaagtatt ctggcgaaag
661 cccatctttt gaaatttcat caaataagcc accattgaac ggaaatctgg aacgccactt
721 gcgacagata caccagcacc tgtaaaaaat gtaatacgat tcgaactatc tataatatgt
781 tttagtgtct ctaaatcatg tttcaagtta aacaccccat caaaataatg aatgatttat
841 aacgtctttt acttttatta tagtatattc attttacgat tatcaaaagc gcagtttaaa
901 cagttctttt taaaaatgaa ttatacatat aatcgaacag ttaaattata ttagttttta
961 cgcaattttt attatagaat aagcatgtat attaaaattg atgtctaaac ataaaggata
1021 atgacccatc gcctaaataa aggaggcaat tttacattga ataaacatga acgtttggac
1081 gaaattgcta aactagtgaa taaaaagggc acgataagaa cgaatgaaat cgtcgaaggt
1141 ttaaatgtgt ctgatatgac agttcgaaga gatttaattg aattggaaaa taaagggatt
1201 ttaacgaaga ttcatggtgg tgcacgcagt aattcaacat ttcagtataa agaaatatcg
1261 cataaagaaa aacatacccg tcaaattgca gaaaaacgat ttatagctaa aaaagctgca
1321 tcattaattg aagatgggga tactttgttt ttcggaccag gaacaacagt ggaactatta
1381 gcagaagaag tcaatcatca tacgctcaca attattacga attgtttgcc ggtgtataaa
1441 attttgttag aaaaacaaac agcacatttt cgtgtctatt taattggtgg tgaaatgcgc
1501 catattacag aagcatttgt aggtgaaatg gcgaacgcca tgttggaaaa actaagattt
1561 agcaagatgt tctttagtag taacgcagta aataaaggtg ctgtgatgac atctacatta
1621 gatgaagcct atacgcaaca actggcacta agtaattcaa ttgaaaaata cttgttaatc
1681 gatcatacga aagttggcaa agaagatttt acatcatttt gtcagttaaa cgaattgact
1741 gcagtggtca tggactatga agatgaagaa aaagtagaaa cgattaaaac atatattgaa
1801 gtagtcgact aaattaaaag tataactgca ttgttaaaat atgtatctat atgctcataa
1861 gtgaatgtga atttgcattc atttatgggc tattttttat gcaaatttat gtcaacggga
1921 acctcgacaa agttttacta caatttcaga cgaataccta attaaaacaa caccaacaat
1981 atgaatgttc acttattgtt tgaaaatacg tcataaacaa acatattata aacatattat
2041 gttgatttat tgtttgttta tgtttataat ttaaacgtaa tcaaattgaa agccttttct
2101 caagattttc ataacctaca aataaatatg tcacatttta agaaaggtat ttcaaaatta
2161 aagtaaaaag gagtcttatt atggcgatta ttattggttc agatgaagct ggcaaacgat
2221 taaaagaagt catcaaatca tacttattag acaataaata tgatgttgtt gacgtaacag
2281 aaggacagga agttgacttt gttgatgcaa ctttggctgt agcaaaagat gttcaaagtc
2341 aagaaggtaa cttaggtatt gttatcgat
//