GenBank-Updates@genbank.bio.net (09/07/90)
LOCUS RHANIFA 2809 bp ds-DNA BCT 07-SEP-1990
DEFINITION A.caulinodans nitrogen fixation protein (nifA) gene, complete cds.
ACCESSION X08014 M37305
KEYWORDS nitrogen fixation protein; regulatory protein.
SOURCE A.caulinodans (strain ORS571) DNA.
ORGANISM Azorhizobium caulinodans
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Rhizobiaceae.
REFERENCE 1 (bases 1 to 2809)
AUTHORS Stein,P.A.
JOURNAL Unpublished (1988) see COMMENT for author address
STANDARD simple automatic
REFERENCE 2 (bases 421 to 2809)
AUTHORS Nees,D.W., Stein,P.A. and Ludwig,R.A.
TITLE The azorhizobium caulinodans nifA sequence: identification of
upstream activating sequences including a new element the anaerobox
JOURNAL Nucleic Acids Res. 16, 9839-9853 (1988)
STANDARD simple automatic
COMMENT Submitted (30-JUN-1988) on tape to the EMBL data library by
P.A.Stein.
FEATURES from to/span description
pept 654 2495 nitrogen fixation protein (nifA)
site 421 660 promoter region
signal 507 520 anaerobox
binding 533 555 gpNifA binding site
site 611 626 gpNtrA consensus sequence
binding 643 646 rRNA binding site
BASE COUNT 469 a 996 c 908 g 436 t
ORIGIN
1 gaacagcagc tctacgggct gctggctgcg gctacaccac gcgcaacggc acggtcgcgc
61 aggcactgga ggcagaggcc gccccccacc tttcgccggc cgcgctcgac gcggcgaagg
121 cggcggcctc catcatggcc atgaacaacg tctactatcg atttacgcat ctggcctcca
181 acaaggccta tgagaccctg ccggcgaagc tccgcatgag cgtcatcggc aatcccggcg
241 tggacaaggt ggacttcgaa ctctggtcgc tcgccgtctc ggccatgaac ggatgcggcc
301 gctgcatcga tgcccatgag gcggtgctgc gcgaggcggg cctcagcgag gcccagatcc
361 agacggcggt gcgcgtcggc gccatcatcg cctccgccgc ggtggccctc gaggccgccg
421 gcgcgggttt tccggaagct gcggaataag cgcggccctg cctccgtccc cgccgccagg
481 gcttcgggga cggatacctt caaaatttga tccagatcaa agccgtccgt tgtctccgcg
541 gtttccatta taacaattcc attaaatcga cggcccggca cacgaggccg cttcagggaa
601 caccgacagg aggctgatcc ctcgcagccg cttttgcggc agggagcatg ccaatgaccg
661 acgccttcca ggtccgcgta cctcgggttt cgtcgagcac cgccggagac atcgccgcgt
721 catccatcac cacgcggggc gcgctgccgc gcccgggagg gatgcctgtg tccatgtcgc
781 gggggacctc gcccgaggtg gcactcatcg gggtctatga gatatcgaag atcctgacgg
841 cgccccggcg cctcgaagtc acgctcgcca atgtggtgaa cgtgctctcc tccatgctgc
901 agatgcggca tggcatgatc tgcatcctcg acagcgaggg cgatcccgac atggtggcca
961 ccaccggctg gacgcctgag atggcgggcc agatccgcgc gcatgtgccc cagaaggcca
1021 tcgaccagat cgtcgccacg cagatgccgc tggtggtgca ggacgtgacg gccgatccgc
1081 tcttcgccgg tcacgaggat ctgttcggcc cgcctgagga ggccaccgtc tccttcatcg
1141 gcgtgccgat caaggccgac caccatgtga tgggcaccct ctccatcgac cgcatctggg
1201 acggcaccgc ccgtttccgc ttcgacgagg acgtgcgctt cctcaccatg gtggccaatc
1261 tcgtcggcca gaccgtgcgc ctgcacaagc tggtggcgag cgaccgcgac cggctgatcg
1321 cccagacgca ccgcctcgaa aaggcgctgc gggaagaaaa atccggggcc gagccggagg
1381 tggccgaggc cgccaacgga tccgccatgg gcatcgtggg cgatagcccg ctggtgaaac
1441 gcctgatcgc gaccgcgcaa gtggtcgccc gctcaaactc caccgtgctg ctgcgcgggg
1501 agagcggcac cggcaaggag ttgttcgccc gtgccatcca cgaactgtcg ccccgcaagg
1561 gcaagccctt cgtgaaggtg aactgcgccg ccctcccgga atcggtgctg gaatcggaac
1621 tgttcggcca tgagaagggc gccttcaccg gtgcgctgaa catgcgccag ggccgcttcg
1681 agctggcgca cggcggcacg ctcttccttg acgagatcgg cgagatcacc cccgctttcc
1741 aggccaagct gctgcgcgtg ctgcaggaag gcgagttcga gcgggtcggc ggcaatcgca
1801 cgctgaaggt ggatgtgcgg ctcgtgtgcg ccaccaacaa gaatctggaa gaggcggtct
1861 ccaagggcga gttccgggcc gatctctact accgcatcca tgtggtgccg ctgatcctgc
1921 cgccgctgcg cgaacggccg ggcgacattc ccaagctcgc gaagaacttc ctcgaccgct
1981 tcaacaagga aaacaagctc cacatgatgc tctcggcgcc ggccatcgac gtgctgcggc
2041 gctgctattt cccgggcaac gtgcgcgagc tggagaactg tatccggcgg acggcaacgc
2101 tcgcccacga tgccgtcatc accccccatg acttcgcctg cgacagcggc cagtgcctct
2161 cggccatgct ctggaagggc tcggccccga agcctgtgat gccgcacgtg ccgccggcgc
2221 ccacgccgct gactccgctc tcccctgctc cgctcgcgac cgcagcgccc gctgcggcga
2281 gcccggcgcc ggcggccgac agcctgccgg tcacttgccc cggcaccgag gcctgtcccg
2341 cggtgccccc ccgccagagc gaaaaggagc agttgctcca ggccatggag cgctccggct
2401 gggtgcaggc gaaggccgcg cgcctcctca acctcacgcc gcgccaggtg ggttatgcgc
2461 tgcgcaaata tgacatcgac atcaagcgct tctgaaccac ggggcggagc ttgctccgcc
2521 ctgtcccgtc cggccgccga tcgcggcgcc cgcgggaaag cgccgggccg ttttatggga
2581 gcccccctcc catgagcggt gagcacacgg caccggagcc cgccgcgccg ccggatccgc
2641 cgggaagctt cgaggcgctg gactggcagg gacgccccgt gcgctgcgag gattgcccgc
2701 acgaggacat tcaggccatc ggccgctgcg atctcggcaa ggtctgcgtg gccgaccggc
2761 gtacccgtcg gatcgaccgc ttcttcgccc gcgaatccgg aactggcgg
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