GenBank-Updates@genbank.bio.net (09/07/90)
LOCUS YSCAGA1A 6057 bp ds-DNA PLN 07-SEP-1990
DEFINITION S.cerevisiae alpha-agglutinin (AG-alpha-1) gene.
ACCESSION M28164
KEYWORDS alpha-agglutinin; cell surface glycoprotein.
SOURCE S.cerevisiae (strain W303-1B) DNA, clone pL-alpha-21.
ORGANISM Saccharomyces cerevisiae
Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
Endomycetales; Saccharomycetaceae.
REFERENCE 1 (bases 1 to 6057)
AUTHORS Lipke,P.N., Wojciechowicz,D. and Kurjan,J.
TITLE AG-alpha-1 is the structural gene for the Saccharomyces cerevisiae
alpha-agglutinin, a cell surface glycoprotein involved in cell-cell
interactions during mating
JOURNAL Mol. Cell. Biol. 9, 3155-3165 (1989)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable copy of sequence [1] kindly
provided by J.Kurjan, 31-OCT-1989.
FEATURES from to/span description
pept 3653 5605 alpha-agglutinin
pept 2591 1941 (c) unidentified open reading frame I
pept 1947 490 (c) unidentified open reading frame I
signal 3319 3294 (c) MAT-alpha-1 control consensus (P/Q box)
signal 3093 3099 pheromone response consensus
signal 3217 3223 pheromone response consensus
signal 3531 3538 TATA box
BASE COUNT 1826 a 1180 c 1063 g 1988 t
ORIGIN
1 aagctttagg taagggaggc agggggaaaa gatactgaaa tgacggaaaa cgagaatatg
61 gagcagggag caacttttag agctttaccc gttaaaaggt caaatcgagg cttcctgcct
121 ttgtctgatt ttagtagtac cggaaggttt attacgccca agaacagtgc ttgaattgag
181 ttctcgggac acgggaaaga caatggaaga aaaatttaca ttcagtagcc ttatatatga
241 aatgctgcca agccacgtct ttataagtag ataatgtccc atgagctgaa ctatgggaat
301 ttatgacgca gttcattgta tatatattac attaactctt tagtttaaca tctgaattgt
361 tttataaaat aactttttga atttttttat gatcgcttag ttaagtctat tatatcaggt
421 tttttcattc atcataattg ttcgttaaat atgagtatat ttaaatacag gaattagtat
481 catttgcagt cacgaaaagg gccgtttcat agagagtttt cttaataaag ttgagggttt
541 ccgtgatagt tttgaggggt tgtttgaact agatttacgc ttacctttca actgattaat
601 tttttcagcg ggcttatcat aatcatccat catagcagtc tttctggact tcgtcgagga
661 ctggctttct gaattttgac ggtccctatt agctccagtt ggaggaattg agttacctac
721 aactggcaag aggtctttgt ttggattcaa aataggactt tgtggtagca gtttggtttt
781 attcaatcta aagatatgag aaacaggttt taagtaaatc gatactattg taccaatgtt
841 tagctccaat tcctccaaaa cggtgggatc taattttgtg ttcatttcta ttagtggcaa
901 ctctccgtcc agtactgatt ttaaagattc aaaagttatc gcgtttgata tacgagacgt
961 tttcgttaat gacagcaatc tccaatacat cagtgtttta tctcttaagt caggattatt
1021 ttcgtgatcg gtgcatcctt ttaataaatc catacaaagt tcttcagttt cctttgtagg
1081 atttctgatg aagaatttta ttgctgagtt cagaatggaa aattgcactt ctagcgtctc
1141 attaaacatg tttgaggaaa aaactctaaa taactccagg tagtttggaa ttacatccga
1201 atattgcgtt attatccaga tcatagcgtt ttttgattca ggttcctgta caacttcagt
1261 gtgtttgact agttctgtta cgtttgcttt aaaattattg ggatatttcc tcaaaatatt
1321 tctgaaaacc gaaataatct cctggacgac ataatcaaca ccgaattcta acaaatctag
1381 tagcacagcg acacaatcgt gtacagagtc ttcatctagc ttaacagcga gattaccaat
1441 ggctctgact gatttccttg acatttgaat atcaatatct gtagcatatt gttccaactc
1501 ttctagaatt cttggtaatg tttccttgtt agctaaaaga tataaacact ctaatttcgt
1561 gtctttgatg tatatggggt cattgtactc gatgaaaaaa tacgaaatgt ctagcctgag
1621 tagagatgac tccctactca ataaaagaag aataacgttt cttaatacta aaaattgtaa
1681 ttcaggcggc ttatctaaca aagctattac agagttagat agcttttcgg ctagagtttc
1741 tttgatgacg tcaacataat tcaacaagta catgatgaat tttaaagagt tcaacactac
1801 gtatgtgttt acttgttgca ggtacggtaa agctagttcg atcatttcat gggtatccaa
1861 ataatgctgc ggcacaaccg aagtcgtcaa aacttccaaa acagtagcct tattccactc
1921 atttaattcg ggtaaaagtt ctagcatgtc aaaagcgagt tccaagggaa tcctgaaggt
1981 tccatgttag cgtttttttc gtgaatggaa tataaagtat gtaatgcagc tacaatgact
2041 tctggagagc tcgactgtgc ctttacaatg tcatgtagaa tgcttgataa ccccaatacc
2101 ctttcatgat caatttcatc taaatccaac agtgcgtaaa ttgctgtcct cgtcacttgt
2161 tcaggtggag acttgtgatt taccaatgaa atgatacagt cgaaggcctg atcagatagc
2221 tctttcaccg ggactaatac cagagttctt agtgccatta tttgtaactt ttcatctctg
2281 cttttgaaat cgtccattat aaatggcaaa gcctctctgg cctgctgagg ttttaatgcg
2341 ccgatcaccc taatatactc atggcaaatt cttttcactt ctagatcatc ttcaatttgc
2401 caaaatttca agagctcaga aaacagaagg gacatttcgc catagtttcc tagaaccaaa
2461 ttggcgataa tttttctcag agcatttttc cttcttgtta tattcgattt aaactttttt
2521 actccaaaat gttgcagatc tgtgacgatt tcatttgctt tatatctggc aaaaactttt
2581 tgatcggaca taagcgaaat acgtcctatt aatgaagtga atgttcttgc tgtattccct
2641 tcttgtgcag tagattaatt ctgtttccag gctgcgatac tttgataccc aatactaaaa
2701 gttgatgatt tgaacgatct cctatttcct cgcacatttt tggagcgata cccggaagac
2761 agaatcgcga tgttaagaaa atagttctga tggcactaaa gagatcatga ttaaggaaag
2821 gtaagtgata tgcatgaatg ggaataggct ttcgaacttg acgatttagt tccttatttc
2881 tatccatcta atcctccaac ttcaataggc cttatctagc tcagagcagt atttaattga
2941 gaatagtagc ttaattgaaa ccttactaaa aaagtgtatg gttacataag ataaggcgtt
3001 aagaagagta tacatatgca ttattcatta ccaagaccac tatgaatagt aataccatat
3061 ttagcttttg aaactcatgt tttctattgt gttgtttcaa attcctctgt taggctcaat
3121 ttaggttaat taaattataa aaaaatataa aaaataaaga aagtttatcc atcggcacct
3181 caattcaatg gagtaaacag tttcaacact gagtggtgaa acattgaaca actacatgca
3241 gtttcccgcc acgaggcaag tgtaggtcct ttgtccattt cgctttgttt tgcaggtcat
3301 tgatgaccta attaggaagg tagaagccgc tccagctcaa taaggaaatg ctaagggtac
3361 tcgcctttgg tgttttacca tacaatggca gctttatgtc acttcattct tcagtaacgg
3421 cgcttaaata ttcccaaaaa cgttacaatg gaattgtttg atcatgtaac gaaatgcaat
3481 cttctaaaaa aaaagccatg tgaatcaaaa aaagattcct tttagcatac tataaatatg
3541 caaaatgccc tctatttatt ctagtaatcg tccattctca tatcttcctt atatcagtcg
3601 cctcgcttaa tatagtcagc acaaaaggaa caacaattcg ccagttttca aaatgttcac
3661 ttttctcaaa attattctgt ggcttttttc cttggcattg gcctctgcta taaatatcaa
3721 cgatatcaca ttttccaatt tagaaattac tccactgact gcaaataaac aacctgatca
3781 aggttggact gccacttttg attttagtat tgcagatgcg tcttccatta gggagggcga
3841 tgaattcaca ttatcaatgc cacatgttta taggattaag ctattaaact catcgcaaac
3901 agctactatt tccttagcgg atggtactga ggctttcaaa tgctatgttt cgcaacaggc
3961 tgcatacttg tatgaaaata ctactttcac atgtactgct caaaatgacc tgtcctccta
4021 taatacgatt gatggatcca taacattttc gctaaatttt agtgatggtg gttccagcta
4081 tgaatatgag ttagaaaacg ctaagttttt caaatctggg ccaatgcttg ttaaacttgg
4141 taatcaaatg tcagatgtgg tgaatttcga tcctgctgct tttacagaga atgtttttca
4201 ctctgggcgt tcaactggtt acggttcttt tgaaagttat catttgggta tgtattgtcc
4261 aaacggatat ttcctgggtg gtactgagaa gattgattac gacagttcca ataacaatgt
4321 cgatttggat tgttcttcag ttcaggttta ttcatccaat gattttaatg attggtggtt
4381 cccgcaaagt tacaatgata ccaatgctga cgtcacttgt tttggtagta atctgtggat
4441 tacacttgac gaaaaactat atgatgggga aatgttatgg gttaatgcat tacaatctct
4501 acccgctaat gtaaacacaa tagatcatgc gttagaattt caatacacat gccttgatac
4561 catagcaaat actacgtacg ctacgcaatt ctcgactact agggaattta ttgtttatca
4621 gggtcggaac ctcggtacag ctagcgccaa aagctctttt atctcaacca ctactactga
4681 tttaacaagt ataaacacta gtgcgtattc cactggatcc atttccacag tagaaacagg
4741 caatcgaact acatcagaag tgatcagtca tgtggtgact accagcacaa aactgtctcc
4801 aactgctact accagcctga caattgcaca aaccagtatc tattctactg actcaaatat
4861 cacagtagga acagatattc acaccacatc agaagtgatt agtgatgtgg aaaccattag
4921 cagagaaaca gcttcgaccg ttgtagccgc tccaacctca acaactggat ggacaggcgc
4981 tatgaatact tacatcccgc aatttacatc ctcttctttc gcaacaatca acagcacacc
5041 aataatctct tcatcagcag tatttgaaac ctcagatgct tcaattgtca atgtgcacac
5101 tgaaaatatc acgaatactg ctgctgttcc atctgaagag cccacttttg taaatgccac
5161 gagaaactcc ttaaattcct tctgcagcag caaacagcca tccagtccct catcttatac
5221 gtcttcccca ctcgtatcgt ccctctccgt aagcaaaaca ttactaagca ccagttttac
5281 gccttctgtg ccaacatcta atacatatat caaaacggaa aatacgggtt actttgagca
5341 cacggctttg acaacatctt cagttggcct taattctttt agtgaaacag cactctcatc
5401 tcagggaacg aaaattgaca cctttttagt gtcatccttg atcgcatatc cttcttctgc
5461 atcaggaagc caattgtccg gtatccaaca gaatttcaca tcaacttctc tcatgatttc
5521 aacctatgaa ggtaaagcgt ctatattttt ctcagctgag ctcggttcga tcatttttct
5581 gcttttgtcg tacctgctat tctaaaacgg gtactgtaca gttagtacat tgagtcgaaa
5641 tatacgaaat tattgttcat aattttcatc ctggctcttt ttttcttcaa ccatagttaa
5701 atggacagtt catatcttaa actctaataa tacttttcta gttcttatcc ttttccgtct
5761 caccgcagat tttatcatag tattaaattt atattttgtt cgtaaaaaga aaaatttgtg
5821 agcgttaccg ctcgtttcat tacccgaagg ctgtttcagt agaccactga ttaagtaagt
5881 agatgaaaaa atttcatcac catgaaagag ttcgatgaga gctacttttt caaatgctta
5941 acagctaacc gccattcaat aatgttacgt tctcttcatt ctgcggctac gttatctaac
6001 aagaggtttt actctctcat atctcattca aatagaaaga acataatcaa aaagctt
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