GenBank-Updates@genbank.bio.net (09/07/90)
LOCUS PB0KANRCG 4545 bp ds-DNA circular BCT 07-SEP-1990
DEFINITION Plasmid pUB110 (from S.aureus) kanamycin nucleotidyl transferase
DNA, complete cds.
ACCESSION M37273
KEYWORDS circular; complete genome; kanamycin nucleotidyl transferase;
kanamycin resistance.
SOURCE Plasmid pUB110 (from Staphylococcus aureus) DNA.
ORGANISM Plasmid pUB110
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 4545)
AUTHORS Bashkirov,V.I., Mil'shina,N.V. and Prozorov,A.A.
TITLE Nucleotide sequence and functional map of the kanamycin-resistant
plasmid pUB110 from Staphylococcus aureus
JOURNAL Genetika 22, 823-831 (1986)
STANDARD simple staff_entry
FEATURES from to/span description
pept 287 1 (c) protein K1
4545 3570 (c) protein K1
pept 1195 944 (c) protein D (gtg start codon)
pept 2173 1412 (c) kanamycin nucleotidyl transferase
pept 3325 2342 (c) protein K2
signal 3335 3398 transcription termination signal
signal 3536 3566 transcription termination signal
BASE COUNT 1306 a 1002 c 622 g 1615 t
ORIGIN
1 ggatccagtt gctcaaaaaa atctcggtca gatgttacta gcaactcatt tacaagaaca
61 gcatctttcc tcgtttttct tgtacctgtt ttttgtgatt caataatttc tttgacacgt
121 tcgttgtaat caatattttt atcatttttc aaatcataat tttcacgtgt tcgctcatgg
181 tcaatatcat cattcgttct actttttcgc tctctttgat tatgaaattg catgcctttt
241 agtccagctg atttcacttt ttgcattcta caaactgcat aactcatatg taaatcgctc
301 ctttttaggt ggcacaaatg tgaggcattt tcgctctttc cggcaaccac ttccaagtaa
361 agtataacac actatacttt atattcataa agtgtgtgct ctgcgaggcg cggcagtgcc
421 gaccaaaacc ataaaacctt taagaccttt ctttttttta cgagaaaaaa gaaacaaaaa
481 aacctgccct ctgccacctc agcaaagggg ggttttgctc tcgtgctcgt ttaaaaatca
541 gcaagggaca ggtagtattt tttgagaaga tcactcaaaa aatctccacc tttaaaccct
601 tgccaatttt tattttgtcc gttttgtcta gcttaccgaa agccagactc agcaagaata
661 aaatttttat tgtctttcgg ttttctagtg taacggacaa aaccactcaa aataaaaaag
721 atacaagaga ggtctctcgt atcttttatt cagcaatcgc gcccgattgc tgaacagatt
781 aataatagat tttagctttt tatttgttga aaaaagctaa tcaaattgtt gtcgggatca
841 attactgcaa agtctcgttc atcccaccac tgatctttta atgatgtatt ggggtgcaaa
901 atgcccaaag gcttaatatg ttgatataat tcatcaattc cctctacttc aatgcggcaa
961 ctagcagtac cagcaataaa cgactccgca cctgtacaaa ccggtgaatc attactacga
1021 ggcgccagcc ttcatcactt gcctcccata gatgaatccg aacctcatta cacattagaa
1081 ctgcgaatcc atcttcatgg tgaaccaaag tgaaacctac tttatcccaa taaaaaccta
1141 tactcttttt aatatccccg actggcaatg ccgggataga ctgtaacatt ctcacgcata
1201 aaatcccctt tcattttcta atgtaaatct attaccttat tattaattca attcgctcat
1261 aattaatcct ttttcttatt acgcaaaatg gcccgattta agcacaccct ttattccgtt
1321 aatgcgccat gacagccatg ataattacta atactaggag aagttaataa atacgtaacc
1381 aacatgatta acaattatta gaggtcatcg ttcaaaatgg tatgcgtttt gacacatcca
1441 ctatatatcc gtgtcgttct gtccactcct gaatcccatt ccagaaattc tctagcgatt
1501 ccagaagttt ctcagagtcg gaaagttgac cagacattac gaactggcac agatggtcat
1561 aacctgaagg aagatctgat tgcttaactg cttcagttaa gaccgaagcg ctcgtcgtat
1621 aacagatgcg atgatgcaga ccaatcaaca tggcacctgc cattgctacc tgtacagtca
1681 aggatggtag aaatgttgtc ggtccttgca cacgaatatt acgccatttg cctgcatatt
1741 caaacagctc ttctacgata agggcacaaa tcgcatcgtg gaacgtttgg gcttctaccg
1801 atttagcagt ttgatacact ttctctaagt atccacctga atcataaatc ggcaaaatag
1861 agaaaaattg accatgtgta agcggccaat ctgattccac ctgagatgca taatctagta
1921 gaatctcttc gctatcaaaa ttcacttcca ccttccactc accggttgtc cattcatggc
1981 tgaactctgc ttcctctgtt gacatgacac acatcatctc aatatccgaa tagggcccat
2041 cagtctgacg accaagagag ccataaacac caatagcctt aacatcatcc ccatatttat
2101 ccaatattcg ttccttaatt tcatgaacaa tcttcattct ttcttctcta gtcattatta
2161 ttggtccatt cactattctc attccctttt cagataattt tagatttgct tttctaaata
2221 agaatatttg gagagcaccg ttcttattca gctattaata actcgtcttc ctaagcatcc
2281 ttcaatcctt ttaataacaa ttatagcatc taatcttcaa caaactggcc cgtttgttga
2341 actactcttt aataaaataa tttttccgtt cccaattcca cattgcaata atagaaaatc
2401 catcttcatc ggctttttcg tcatcatctg tatgaatcaa atcgccttct tctgtgtcat
2461 caaggtttaa ttttttatgt atttctttta acaaaccacc ataggagatt aaccttttac
2521 ggtgtaaacc ttcctccaaa tcagacaaac gtttcaaatt cttttcttca tcatcggtca
2581 taaaatccgt atcctttaca ggatattttg cagtttcgtc aattgccgat tgtatatccg
2641 atttatattt atttttcggt cgaatcattt gaacttttac atttggatca tagtctaatt
2701 tcattgcctt tttccaaaat tgaatccatt gtttttgatt cacgtagttt tctgtattct
2761 taaaataagt tggttccaca cataccaata catgcatgtg ctgattataa gaattatctt
2821 tattatttat tgtcacttcc gttgcacgca taaaaccaac aagattttta ttaatttttt
2881 tatattgcat cattcggcga aatccttgag ccatatctga caaactctta tttaattctt
2941 cgccatcata aacattttta actgttaatg tgagaaacaa ccaacgaact gttggctttt
3001 gtttaataac ttcagcaaca accttttgtg actgaatgcc atgtttcatt gctctcctcc
3061 agttgcacat tggacaaagc ctggatttac aaaaccacac tcgatacaac tttctttcgc
3121 ctgtttcacg attttgttta tactctaata tttcagcaca atcttttact ctttcagcct
3181 ttttaaattc aagaatatgc agaagttcaa agtaatcaac attagcgatt ttcttttctc
3241 tccatggtct cacttttcca ctttttgtct tgtccactaa aacccttgat ttttcatctg
3301 aataaatgct actattagga cacataatat taaaagaaac ccccatctat ttagttattt
3361 gtttagtcac ttataacttt aacagatggg gtttttctgt gcaaccaatt ttaagggttt
3421 tcaatacttt aaaacacata cataccaaca cttcaacgca cctttcagca actaaaataa
3481 aaatgacgtt atttctatat gtatcaagat aagaaagaac aagttcaaaa ccatcaaaaa
3541 aagacacctt ttcaggtgct ttttttattt tataaactca ttccctgatc tcgacttcgt
3601 tcttttttta cctctcggtt atgagttagt tcaaattcgt tctttttagg ttctaaatcg
3661 tgtttttctt ggaattgtgc tgttttatcc tttaccttgt ctacaaaccc cttaaaaacg
3721 tttttaaagg cttttaagcc gtctgtacgt tccttaagga attccttagt gctttcatag
3781 attaaactca catcacgctt taaatcgctt attttagact ttaaagactt gttttcttca
3841 agcaactcat tataatcatt tacattttca ttaaatcgct ctacaagacc actatatttt
3901 tctttaactt gcccatgttc tttacttaat tttttatatt ctctcgccat atcagtactc
3961 atgagatttc taacatgctg ttttaaccta tcgttatctc tcgcagcagt cactaagttt
4021 ttataatcac gctccgatat aacaacattt ttggttggtt tcttttctgt tttcattatt
4081 tcttttccca aaccaaacat ggacttttca cccgttggca cttcaacact tttcatgtgt
4141 cgtttcgctg gtacttctaa atctgattta actttatcgc tataagcagt ccattcatct
4201 tttttaactg ctaaattttt ttctagaaaa tcaatctctt tttccaaagt ttgtttttta
4261 aatttagctg tctcaatatg tttacggtca gagccacgtt caccacgctt caactcaaaa
4321 ccctgttttt tcatatgctc ggggaattta tcttgtagcc ataacagttc ttgacgatta
4381 aacacatttt ttccttgcag ttttccatca cgcataggca caacacctaa atgcatgtga
4441 ggggtttgct catcattatg aactgttgca taagcaatat tttgcttgcc atatcgttcg
4501 gaaaataatt tataactttc ctcaaaaaat cgtttttgtt ctcct
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