GenBank-Updates@genbank.bio.net (09/07/90)
LOCUS BOVCASKA3 7595 bp ds-DNA MAM 07-SEP-1990
DEFINITION Bovine kappa-casein gene, exons 3-5.
ACCESSION X14908 X14326 M36906
KEYWORDS kappa-casein; repetitive sequence: Alu-like repetitive sequence;
restriction fragment linked polymorphism.
SEGMENT 3 of 3
SOURCE Bovine DNA.
ORGANISM Bos taurus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Artiodactyla; Ruminantia; Pecora; Bovidae.
REFERENCE 1 (bases 1 to 7595)
AUTHORS Mackinlay,A.G.
TITLE ;
JOURNAL Unpublished (1989) see COMMENT for author address
STANDARD simple automatic
REFERENCE 2 (bases 1 to 7595)
AUTHORS Alexander,L.J., Stewart,A.F., Mackinlay,A.G., Kapelinskaya,T.V.,
Tkach,T.M. and Gorodetsky,S.I.
TITLE Isolation and characterization of the bovine kappa-casein gene
JOURNAL Eur. J. Biochem. 178, 395-401 (1988)
STANDARD simple automatic
COMMENT *source: library=EMBL3, EMBL4.
EMBL features not translated to GenBank features:
key from to description
MSG 4930 5446 exon 4
VARIANT 5309 5309 c is t in kappa-casein B variant;
changes acc (Thr) to atc (Ile)
VARIANT 5345 5345 a is c in kappa-casein B variant;
changes gat (Asp) to gct (Ala)
VARIANT 5406 5406 a is g in kappa-casein B variant;
loss of PstI site
VARIANT 5413 5413 a is t in kappa-casein B variant
MSG 7296 7416 exon 5
VARIANT 7382 7382 t is c in kappa-casein B variant;
loss of BglII site, creates MspI
site
POLYA 7416 7416 polyA site
VARIANT 7484 7484 t is c in variant clone
VARIANT 7486 7486 t is c in variant clone
VARIANT 7504 7504 t is c in variant clone
VARIANT 7504 7504 t is c in variant clone
VARIANT 7554 7554 g is a in variant clone
VARIANT 7570 7571 c is inserted in variant clone
[1] Author address: A.G.Mackinlay, The University of New South
Wales, P.O. Box 1 Kensington New South Wales Australia.
Submitted (16-MAR-1989) on tape to the EMBL Data Library
FEATURES from to/span description
pept + 2886 2918 kappa-casein precursor, exon 3
4930 5412 kappa-casein precursor, exon 4
sigp + 2886 2891 kappa-casein signal peptide (AA -2 to -1)
matp 2892 2918 kappa-casein (AA 1-9)
4930 5409 kappa-casein (AA 10-169)
pre-msg < 1 7467 kappa-casein mRNA and introns
IVS < 1 2885 kappa-casein intron B
IVS 2919 4929 kappa-casein intron C
IVS 5447 7296 kappa-casein intron D
BASE COUNT 2646 a 1292 c 1182 g 2475 t
ORIGIN
1 agatctgaca tgactgagcg actgaactga attgatgacc tcctcttaaa ctgtaaaatt
61 tggaacagca ctagtccttg tttcataatt catttattat taaatttagt tttgctttca
121 agttaatatc tcttaaataa ttctatttgg taaattttta gaatcttttt aaaaatttga
181 attactatga ccttcatatt tcttcctttc ttctttgaaa tttgatttta ttgtggtcac
241 ctgcatattt agaacttggg ctttctcttg agggatagag gttaaagttt aaatcagaaa
301 gtgattcaca actgcttgac ctctgaaaac atcttcttga actatttaag ccagtgctat
361 ccccaaactc tccttcctgt cttcacaaag ctacaagccc catggatata aacaaataat
421 tcaaaagaag aatgaaaatt tatataattt ttaccatcca atcttataca atcttactta
481 cccaatcaaa atcatacaac ttaatacagc aatcggagaa ggcaatggca ccccactcca
541 gtactcttgc ctgaaagatc ccatggactg aggagcctgg tagactgcag tccatggggt
601 cactaagagc tcagacacga ctgtgtaact ttcaagtaga catcctgagc gacttcactt
661 tcacttttca ctttcatgca ttggagaagg aaatggcaag ccactccagt gttcttgcct
721 ggagaatccc agggacgggg aagcctggtg cggctgcccg tctctggggt cacacagagt
781 cagacacgac tgaagtgact tagcagcagc agcagcaata cagcaatgtt gtagcatctt
841 ttatgtgcta aatgtattcc ttctctccaa aatataaata actaagataa cacttattac
901 tagcaattac aagatgaact gcacactctt atagtctact gatgacatta taaattggta
961 tatctttact gtacagaagt ttaataattt ctgtattttc cccccaaaat catatttttg
1021 atggactgtt ttaagaaatc actgaaaact atttaaaagg ccattgacat ctgggcttcc
1081 ctggtgtctc agaaagtaaa gaatctgcct gcatgcagga gacctagttt tcatccttgg
1141 attgggaaga tttccccgga gaagggaatg gctacccact caagtatctt acctggagaa
1201 ttcatggaca ggggagcctc atgggctaca gttcataggt cattttaaaa gtccgttgat
1261 atctatccag atgttatcca tgcctgtatt tataataaaa acattttttg ataaaccttt
1321 aaaagttaac agtaaagaac aaacatttca tgacataacc attgattgtt tttcaacttt
1381 gaatatgcaa gtatgaaaac tgaggtgatg gaaaaaatga tatgagatca tatgccttta
1441 aaaaatttaa gatacaagat actaaaatga acatatacca ggaatacata atgcatgtag
1501 actgatatta tataaaatct atatctaaat tttcaggatt ttttagtgta ggcaaacttt
1561 caatattaat ataacttttt aaattttaaa ctaatgactt aataaattta gaggtaaata
1621 agtccctgtc ttaacacacc ctgtatttta ttattttttt taaaatctct gatatcctat
1681 accaacaact tcctaaaggt ttataggatc ttttatatcc caggggatat gtgtgaaagt
1741 cttaaactaa aactgtcatc ttaaaactat ttaccattat gaactcacct tttgtaagtt
1801 ttaaagcttc atatagatat gcctggaaac tactttctca ctcctgattt cctatgcatg
1861 ttacaatcca gaactaagaa aagtactata aatacatgat aattttttaa atgctattat
1921 ttatataaca tttatatacc agtgttatgc tagaggcaat tttttaatca agcactggat
1981 ggatggattt ttaatcaacc agatggatga tccggggagg gaggagggag agggattcag
2041 gatggggaac atgtgtacac ctgtagtgga ttcatgttga tgtatggcaa aaccaataca
2101 atattgtaaa gtaattagcc tccaattaaa attaaaaaat tatattaaaa aaaagaaaac
2161 ctcataaatc atcttagagc agaaacagaa aaatatgcta aattctcctc ttatttttag
2221 caatatatat tcaatacaaa ttgaatatag gttgttcatg ttcttcattt aagcaatatc
2281 atatatagtt gttggtacag agatcaaatg atcatattct tttcaatctt aaatttttct
2341 ccctaaatag tgatttttaa gaacaactta accatggggc tcattattgc tatttaagtt
2401 agcatgtatc tgtcatttct tgagggtttc acagactatc tgctaagtca cttcagtcgt
2461 gtccagctct gtgcgacccc atagacggca gcccaccagg ctctcccgtc tctgggattc
2521 tccaggcaag aacactggag tgggttgcca tttccttctc caatgcatga aagtgaaaag
2581 tgaaagtgaa gtcactcagt catgttcgac acttagtgat cccatggact gcagcccacc
2641 aggctcctcc atgcatggga ttttccaggc aagagtactg gagtggggtg ccattgcctt
2701 ctctgtcaca gactatagat aacctagaaa agtgctttat aatattttac aatactaaaa
2761 taaaagcaac aaaaacatgt tttatctttt ttataaaaga tatttttaaa tcacaaaaac
2821 tagactctat catgcttttt ttcttctaac ttcttctttc tgattctttc tctgaatctc
2881 aacagggtgc ccaggagcaa aaccaagaac aaccaatagt aagtttgttt ccagtagaga
2941 tatgaatcca aagaataaaa ttctgcaaag tttaatgata ttaaaatgcc ttctatcaaa
3001 agactaagta aatatttaaa ctacataaaa tagttgaata atctttgtaa aaataatcat
3061 ttcagcaacc actcatatga atccacatca atgtttatct aaaatcttgg tatcacttta
3121 caggccaagt cgtgttgatt ttcatgagta tttagcaaca tgagaaaacc ttctcagaat
3181 ttaatactgg gaaaacaatg aaaacaaaag caaagcttaa tatttcttct gtctactttt
3241 taagaaatgg ggaatgatcc ctctctgaaa catcttcaga tgcttctcat acaattgtat
3301 tcattccccc agtttgtttg aatgaatacc tttaaatatg tctcctttca tgaggaagcc
3361 ttgctttgag gtataatttc attaatttgt ttctattcat aaggaaagat ctaatgattt
3421 tgtattttat aactgcacct aattaatatt aattaataga actaatcaat tattataatt
3481 agttaattat aatataatat tatatatatc acatgatgtg atgtgatgtg gaatatatat
3541 atttatcata taatgtgata ttatgtgata tatatcacat tatatcacaa tatatcacat
3601 gatgtgatgt ggaatatata ttattatata gttttatata atatatatta ataattgatt
3661 agttctatta ctttggccac ctgatgtgaa gagctgactc atttgaaaag accctgatgc
3721 taggaaagat tgagggcagg aggagaaggg gacgacagag gatgagatgg ttggatggca
3781 tcatggactc aatggacatg ggtctgggtg gactccagga attggtgatg gacagggtgg
3841 cctagtgtgc tgcagttcat ggggtcagaa agagttggac atgactgagc gactgaactg
3901 aactgaactg aatatataat atataaatat tataatatta tatataatat atattataaa
3961 attttataaa ttatataaaa aatatataaa cccaagaatc catatcacat cacatcatta
4021 catataatat ataatataat gtataatata atttataata ttagatgatt atataatata
4081 cagtattaat atattagtat ataatataat tgtatatagt taactgcacc taattaatgt
4141 attttatgac tgcacctaat tgatagaact gtggcaaata aagatggaaa atttccgtaa
4201 ctgattttta acggataaaa ctaagcagaa aaaaaacccc aacatataaa cccaggaatc
4261 cacatcacat tatttttact attttgcttg aattagttta gataaaatgc acccttaacc
4321 taatccctag ataaataaaa taaaaaacag ttacaaacat gtggtgagaa taaacatata
4381 tatatatata tatatacaca cacacacata tacacactca tatatatgga aagagatata
4441 cagttttttt tctttgtgtg tagtatgctt gtgtattgta tgtcaaagct ctctatgaaa
4501 ctggtctagc tgtggtgctt ataaagtgca acatttactg aggcactttt tatatatgcc
4561 aagctctggg caaagtggtt tacattatga gctatctcat ctaatttttt ttgaaactaa
4621 tgttattttt aatatttgct gaaaatcaag aagtggaagg aaaatgtaca aatccatatt
4681 ttataaaata atatggtttc tgggttcact attcccaatg ttgtactttc ttaacatcaa
4741 atactgtaac aatttgtttc aaaaaattct gatttaagat atctcttcac tctgcttctg
4801 ctgctgctaa gtcgcttcag tcctggctga ctcttgcgac cccatagatg gcagcccact
4861 aggctcccca gtccctggga ttctccaggc aagaaataat accattctgc ataatttatt
4921 tttttacagc gctgtgagaa agatgaaaga ttcttcagtg acaaaatagc caaatatatc
4981 ccaattcagt atgtgctgag taggtatcct agttatggac tcaattacta ccaacagaaa
5041 ccagttgcac taattaataa tcaatttctg ccatacccat attatgcaaa gccagctgca
5101 gttaggtcac ctgcccaaat tcttcaatgg caagttttgt caaatactgt gcctgccaag
5161 tcctgccaag cccagccaac taccatggca cgtcacccac acccacattt atcatttatg
5221 gccattccac caaagaaaaa tcaggataaa acagaaatcc ctaccatcaa taccattgct
5281 agtggtgagc ctacaagtac acctaccacc gaagcagtag agagcactgt agctactcta
5341 gaagattctc cagaagttat tgagagccca cctgagatca acacagtcca agttacttca
5401 actgcagtct aaaaactcta aggagacatc aaagaagaca acgcaggtaa ataaggcaaa
5461 atgaataaca gccaagattc atggacttat taataaaatc gtaacatcta aactagcgta
5521 gatggataaa ttaaatctgt tacagagaag gcgaaatggg ctaattataa cttacatttg
5581 ctggttcttt atcatgtata tactagattc tttcccaaca agaaagtttt aaaatatttt
5641 acaaaatgag taaaaattgc agattttatt attaaacctt tttcaacaat tggtatactc
5701 cttgaatcta ttagttttat tttacttctg ttcacacaca aaaacagtaa agtacagtgt
5761 caactcatga tttttcttat ctcaaaaata tgttttctta gaaaaaaatc atataggata
5821 tgagtttaaa tatattttaa ttgtatacca gtgttgttgc actctacttt tggtaagaat
5881 agaatgaaag gaaacaatgt ttcactcata gcattttatc tcaggcatca cttccgaaag
5941 gctgtataag gttaggcacc caaatttctc cctattgtaa ggtaatccca cagaaaaaat
6001 tctaattttt taacttgaaa agcacctact tattaagagg aaactttcag aactttaaat
6061 ataatgtaaa atatgtctat ttattatttg aatcaatgga taatggcaga gtccaaaata
6121 atttaaaaca catgaagagg ttaaacagaa agatcaataa gatagaaaat aactaaagat
6181 tgtgaaataa tttaggaaga aagtgcaaca ttttgagatt ctaggcaaca aatactttca
6241 tcacactgtc tacatgagtt tttggtctaa aatgaactca gcaatgatat aatggtaata
6301 gcaatagatc agagaagcct ggttcttctg ttgtgtgact ttaagcaatt cattactctc
6361 tctaaagagt catcactctc attttctcat aattttaagg ttgctatgag attcaagtga
6421 gaaagcatat tgtgaatgag gtgtaaaaag caatcaatct gtgaaatata atgctctaga
6481 aaagaagtat aattagtaaa tattaaaatt tactttcaac taaaatatca agtccttact
6541 aaattatcaa aactacataa caatctcagt ttaatcctgt agcagccgta tggaatggat
6601 tatattacca tcttaaatcc acaaatgaaa gctatggatc ggaaattgct acaggctgga
6661 aagttcaaaa ctggaaacca aatcaaactt gcttgatttc agatagtaag gcttaattag
6721 tacgtctgct atttccatat gggctatttg ttaaaaaaaa ataaaaataa aaacagaaaa
6781 catactctaa ttttccaaga tcagcatatg tgtaaatttt attactgaga ttgtgtttaa
6841 taggacatct ggtaagaaag agaagacaat aagctgataa atctttgact cattaaaatt
6901 tcaaaaagta aaatgaaaac aagtaattga gtttgcaact atctatttta ttgatatgaa
6961 atacattata tttacatgat tgtttttaat aaataatcat tatctcatag tcacatttgt
7021 tcaaaactca agaacctaga ttcctaagtt aaaaccacac actacttata aaaaaagaat
7081 gtataggact gtgtctttct gtgaatatta gatgaagaca attgtataag atcatcagtt
7141 cactatgtgg tgaatttaaa tatttaaatt tatatattta gttagataaa ctacttggat
7201 acagacatga agcaaatcac agaagctaaa taaaatacat atcatgagaa ctgttagcac
7261 attgtgaact aaatctctaa actgtcattt ttataggtct agctgaaacc aaatgactac
7321 ttcaaacttt cctttggcca gttgtctgct tcggtgaaca gagaatatga ttttcacaga
7381 tctggctcct tcctcgtctc ctcttacatt ttacttttat gccagattta attttttgat
7441 tcctgcataa taaagccaat caaatgcaat ggatctcttt tcttttttaa tttatttatt
7501 ttaattggag gctaattact ttacaatatt gtattggttc taccacacat caacgtgaat
7561 ctgccacggg atacacgtgt tccccatcct gaacc
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