GenBank-Updates@genbank.bio.net (09/07/90)
LOCUS ECOPFL 3592 bp ds-DNA BCT 07-SEP-1990
DEFINITION E. coli pfl gene encoding pyruvate formate-lyase (EC 2.3.1.54).
ACCESSION X08035
KEYWORDS pyruvate formate-lyase.
SOURCE Escherichia coli.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 3592; for [2])
AUTHORS Knappe,J.
TITLE ;
JOURNAL Unpublished (1988) see COMMENT for author address
STANDARD simple automatic
REFERENCE 2 (sites)
AUTHORS Roedel,W., Plaga,W., Frank,R. and Knappe,J.
TITLE Primary structures of Escherichia coli pyruvate formate-lyase and
pyruvate-formate-lyase-activating enzyme deduced from the DNA
nucleotide sequences
JOURNAL Eur. J. Biochem. 177, 153-158 (1988)
STANDARD simple staff_entry
COMMENT *source: strain=K12;
EMBL features not translated to GenBank features:
key from to description
PRM 18 23 put.-35 region
PRM 42 47 put.-10 region
RBS 84 90 put.rRNA binding site
TERM 2423 2448 pot.stem-loop structure
PRM 2468 2473 put.-35 region
PRM 2480 2485 put.-10 region
RBS 2560 2565 put.rRNA binding site
TERM 3325 3354 pot.stem-loop structure
[1] Author address Knappe J., University of Heidelberg, Institut
fuer Biologische Chemie, Im Neuenheimer Feld 501, 6900 Heidelberg,
FRG.
Submitted (06-JUL-1988) on tape to the EMBL data library.
FEATURES from to/span description
pept 101 2383 pyruvate formate-lyase (AA 1-760)
pept 2575 3315 pyruvate formate-lyase activating enzyme (AA
1-246)
BASE COUNT 945 a 917 c 864 g 866 t
ORIGIN
1 gttgacatac tgggtcattt acctgcgtga aaacgaccac cattaatggt tgtcgaagta
61 cgcagtaaat aaaaaatcca cttaagaagg taggtgttac atgtccgagc ttaatgaaaa
121 gttagccaca gcctgggaag gttttaccaa aggtgactgg cagaatgaag taaacgtccg
181 tgacttcatt cagaaaaact acactccgta cgagggtgac gagtccttcc tggctggcgc
241 tactgaagcg accaccaccc tgtgggacaa agtaatggaa ggcgttaaac tggaaaaccg
301 cactcacgcg ccagttgact ttgacaccgc tgttgcttcc accatcacct ctcacgacgc
361 tggctacatc aacaagcagc ttgagaaaat cgttggtctg cagactgaag ctccgctgaa
421 acgtgctctt atcccgttcg gtggtatcaa aatgatcgaa ggttcctgca aagcgtacaa
481 ccgcgaactg gatccgatga tcaaaaaaat cttcactgaa taccgtaaaa ctcacaacca
541 gggcgtgttc gacgtttaca ctccggacat cctgcgttgc cgtaaatctg gtgttctgac
601 cggtctgcca gatgcatatg gccgtggccg tatcatcggt gactaccgtc gcgttgcgct
661 gtacggtatc gactacctga tgaaagacaa actggcacag ttcacttctc tgcaggctga
721 tctggaaaac ggcgtaaacc tggaacagac tatccgtctg cgcgaagaaa tcgctgaaca
781 gcaccgcgct ctgggtcaga tgaaagaaat ggctgcgaaa tacggctacg acatctctgg
841 tccggctacc aacgctcagg aagctatcca gtggacttac ttcggctacc tggctgctgt
901 taagtctcag aacggtgctg caatgtcctt cggtcgtacc tccaccttcc tggatgtgta
961 catcgaacgt gacctgaaag ctggcaagat caccgaacaa gaagcgcagg aaatggttga
1021 ccacctggtc atgaaactgc gtatggttcg cttcctgcgt actccggaat acgatgaact
1081 gttctctggc gacccgatct gggcaaccga atctatcggt ggtatgggcc tcgacggtcg
1141 taccctggtt accaaaaaca gcttccgttt cctgaacacc ctgtacacca tgggtccgtc
1201 tccggaaccg aacatgacca ttctgtggtc tgaaaaactg ccgctgaact tcaagaaatt
1261 cgccgctaaa gtgtccatcg acacctcttc tctgcagtat gagaacgatg acctgatgcg
1321 tccggacttc aacaacgatg actacgctat tgcttgctgc gtaagcccga tgatcgttgg
1381 taaacaaatg cagttcttcg gtgcgcgtgc aaacctggcg aaaaccatgc tgtacgcaat
1441 caacggcggc gttgacgaaa aactgaaaat gcaggttggt ccgaagtctg aaccgatcaa
1501 aggcgatgtc ctgaactatg atgaagtgat ggagcgcatg gatcacttca tggactggct
1561 ggctaaacag tacatcactg cactgaacat catccactac atgcacgaca agtacagcta
1621 cgaagcctct ctgatggcgc tgcacgaccg tgacgttatc cgcaccatgg cgtgtggtat
1681 cgctggtctg tccgttgctg ctgactccct gtctgcaatc aaatatgcga aagttaaacc
1741 gattcgtgac gaagacggtc tggctatcga cttcgaaatc gaaggcgaat acccgcagtt
1801 tggtaacaat gatccgcgtg tagatgacct ggctgttgac ctggtagaac gtttcatgaa
1861 gaaaattcag aaactgcaca cctaccgtga cgctatcccg actcagtctg ttctgaccat
1921 cacttctaac gttgtgtatg gtaagaaaac gggtaacacc ccagacggtc gtcgtgctgg
1981 cgcgccgttc ggaccgggtg ctaacccgat gcacggtcgt gaccagaaag gtgcagtagc
2041 ctctctgact tccgttgcta aactgccgtt tgcttacgct aaagatggta tctcctacac
2101 cttctctatc gttccgaacg cactgggtaa agacgacgaa gttcgtaaga ccaacctggc
2161 tggtctgatg gatggttact tccaccacga agcatccatc gaaggtggtc agcacctgaa
2221 cgttaacgtg atgaaccgtg aaatgctgct cgacgcgatg gaaaacccgg aaaaatatcc
2281 gcagctgacc atccgtgtat ctggctacgc agtacgtttc aactcgctga ctaaagaaca
2341 gcagcaggac gttattactc gtaccttcac tcaatctatg taattagatt tgactgaaat
2401 cgtacagtaa aaagcgtaca ataaaggctc cacgaaagtg gggccttttt tagcgcgaga
2461 gccttttttg tcagctatct atactttaag gtgactgcca aaacagactc gacgtagcct
2521 tcgagctgcg caccaacacg gcctcagatg ggccacatct ggagaaacac cgcaatgtca
2581 gttattggtc gcattcactc ctttgaatcc tgtggaaccg tagacggccc aggtattcgc
2641 tttatcacct ttttccaggg ctgcctgatg cgctgcctgt attgtcataa ccgcgacacc
2701 tgggacacgc atggcggtaa agaagttacc gttgaagatt tgatgaagga agtggtgacc
2761 tatcgccact ttatgaacgc ttccggcggc ggcgttaccg catccggcgg tgaagcaatc
2821 ctgcaagctg agtttgttcg tgactggttc cgcgcctgca aaaaagaagg cattcatacc
2881 tgtctggaca ccaacggttt tgttcgtcgt tacgatccgg tgattgatga actgctggaa
2941 gtaaccgacc tggtaatgct cgatctcaaa cagatgaacg acgagatcca ccaaaatctg
3001 gttggagttt ccaaccaccg cacgctggag ttcgctaaat atctggcgaa caaaaatgtg
3061 aaggtgtgga tccgctacgt tgttgtccca ggctggtctg acgatgacga ttcagcgcat
3121 cgcctcggtg aatttacccg tgatatgggc aacgttgaga aaatcgagct tctcccctac
3181 cacgagctgg gcaaacacaa atgggtggca atgggtgaag agtacaaact cgacggtgtt
3241 aaaccaccga agaaagagac catggaacgc gtgaaaggca ttcttgagca gtacggtcat
3301 aaggtaatgt tctaatttcc ccgtaaagcg gcaactttat tgagttgccg cttttttatt
3361 ccgcgcttca ttctgctaca gcgtttttaa cacatcttat agtgcatcta ccatatcccg
3421 tacctgagat aataacgact gataatagct cgcgtgaagt tcttccccat cgaataccac
3481 tgtattacac aagaaagtga catcggcatc ttcaatacca agataactgc acatattttt
3541 taaataatcg cttatatttt tttcccagcc catctggaca aatgattctt tg
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