GenBank-Updates@genbank.bio.net (09/08/90)
LOCUS PT4DOHM 3541 bp ds-DNA PHG 08-SEP-1990
DEFINITION Bacteriophage T4 deoxycytidylate hydroxymethylase gene, complete
cds.
ACCESSION M37159
KEYWORDS DNA polymerase; deoxycytidylate hydroxymethylase.
SOURCE Bacteriphage T4 DNA.
ORGANISM Bacteriophage T4
Viridae; ds-DNA nonenveloped viruses; Myoviridae.
REFERENCE 1 (bases 1 to 3541)
AUTHORS Lamm,N., Wang,Y., Mathews,C.K. and Rueger,W.
TITLE Deoxycytidylate hydroxymethylase gene of bacteriophage T4:
Nucleotide sequence determination and over-expression of the gene
JOURNAL Eur. J. Biochem. 172, 553-563 (1988)
STANDARD simple staff_entry
FEATURES from to/span description
pept < 1 1686 ORF 43
pept 1870 2247 ORF 42.2
pept 2259 2510 ORF 42.1
pept 2664 3404 deoxycytidylate hydroxymethylase
pept 3428 > 3541 beta-gt gene product
signal 1700 1713 pot. transcription termination signal
BASE COUNT 1132 a 547 c 730 g 1132 t
ORIGIN 24.8-28.4 kb on T4 map.
1 atcgatctag ttttaagtat gtcttattac gctaaaatgc ctttttctgg tgtaatgagt
61 cctattaaaa cttgggatgc tattattttt aactcattga aaggtgaaca taaggttatt
121 cctcaacaag gttcgcacgt taaacagagt tttccgggtg catttgtgtt tgaacctaaa
181 ccaattgcac gtcgatacat tatgagtttt gacttgacgt ctctgtatcc gagcattatt
241 cgccaggtta acattagtcc tgaaactatt cgtggtcagt ttaaagttca tccaattcat
301 gaatatatcg caggaacagc tcctaaaccg agtgatgaat attcttgttc tccgaatgga
361 tggatgtatg ataaacatca agaaggtatc attccaaagg aaatcgctaa agtatttttc
421 cagcgtaaag actggaaaaa gaaaatgttc gctgaagaaa tgaatgccga agctattaaa
481 aagattatta tgaaaggcgc agggtcttgt tcaactaaac cagaagttga acgatatgtt
541 aagttcagtg atgatttctt aaatgaacta tcgaattaca ccgaatctgt tctcaatagt
601 ctgattgaag aatgtgaaaa agcagctaca cttgctaata caaatcagct gaaccgtaaa
661 attctcatta acagtcttta tggtgctctt ggtaatattc atttccgtta ctatgatttg
721 cgaaatgcta ctgctatcac aattttcggc caagtcggta ttcagtggat tgctcgtaaa
781 attaatgaat atctgaataa agtatgcgga actaatgatg aagatttcat tgcagcaggt
841 gatactgatt cggtatatgt ttgcgtagat aaagttattg aaaaagttgg tcttgaccga
901 ttcaaagagc agaacgattt ggttgaattc atgaatcagt tcggtaagaa aaagatggaa
961 cctatgattg atgttgcata tcgtgagtta tgtgattata tgaataaccg cgagcatctg
1021 atgcatatgg accgtgaagc tatttcttgc cctccgcttg gttcaaaggg cgttggtgga
1081 ttttggaaag cgaaaaagcg ttatgctctg aacgtttatg atatggaaga taagcgattt
1141 gctgaaccgc atctaaaaat catgggtatg gaaactcagc agagttcaac accaaaagca
1201 gtgcaagaag ctctcgaaga aagtattcgt cgtattcttc aggaaggtga agagtctgtc
1261 caagaatact acaagaactt cgagaaagaa tatcgtcaac ttgactataa agttattgct
1321 gaagtaaaaa ctgcgaacga tatagcgaaa tatgatgata aaggttggcc aggatttaaa
1381 tgcccgttcc atattcgtgg tgtgctaact tatcgtcgag ctgttagcgg tttaggtgta
1441 gctccaattt tggatggaaa taaagtaatg gttcttccat tacgtgaagg aaatccattt
1501 ggtgacaagt gcattgcttg gccatcgggt acagaacttc caaaagaaat tcgttctgat
1561 gtgctatctt ggattgacca ctcaactttg ttccaaaaat cgtttgttaa accgcttgcg
1621 ggtatgtgtg aatcggctgg catggactat gaagaaaaag cttcgttaga cttcctgttt
1681 ggctgataga ataaatctag gacctccagg tccctttttc atacaagtaa tataaatcta
1741 tacttatgaa aaagagatga ttctggaccc tttggattcc ctaaaaattt tttcacaaaa
1801 ctgtttacaa gactgttctt ccatggtact atacaactat caactactga tacggatttg
1861 gagaatgaaa tgaaaatcgc tattttggtt attgcattag gtcttgctgg ttgtgtagct
1921 caaggaccgg tagtaaatca gtctgatgta ggaaaaattg taaactgttc aagcaaattt
1981 tataatccta atgtcaagtg ttataaagaa gctccaaaac agacagtaga acaaatgcag
2041 gcgaattttg acgaagctat tcgtccagat gaatctgctc aagcatatcg taattcagat
2101 gtaattacac gcgaagaaaa aattgaaaac tactgcgctg agctttgggc aaattgggct
2161 aataattacc agtggcgtac aggtaaaaac gctccgatgg agtatgtagt gaattcttat
2221 aattcatgtg taaaaaattt gacctaagtg aggaaaacat ggaaacttta gtagcaggtt
2281 caatttttat ggttttagtt tcaggcgtgt tggctattat tatatacatg cttccatggt
2341 ttatcgcctt gatgcgtggg tcaaaatcga cagtaggaat ctttttcgca tctttactgt
2401 ttaactggtc aattattggt tggtttatta catttatttg gtcaattgca ggtgaaacta
2461 aaaagtctgc gcagccaaat caggtaatta tcatcagaga gaaggaatga aaagcaaaat
2521 tatagcagca ttgcttttaa tcttgatgat tataataagt atatactata gtgtaacggt
2581 tcctcttatg attccaacta ttattttagg ttggagttta ttactgttac aagttaaata
2641 tgaatgtatc aattgaggtt taaatgatta gtgactctat gacagttgaa gaaatccgtc
2701 ttcatttggg gcttgcatta aaagaaaaag atttcgtagt tgataaaaca ggtgttaaaa
2761 ctattgaaat tattggcgca tcatttgtag cagatgaacc gtttattttt ggcgctctta
2821 atgatgaata cattcagcgt gaacttgaat ggtataaatc taaaagcttg ttcgttaaag
2881 atattccggg tgaaacaccg aagatttggc aacaagtagc atcttctaaa ggtgaaatta
2941 actcgaatta tggttgggct atctggtcag aagataacta tgctcagtat gacatgtgtt
3001 tagctgaact tggtcaaaat cctgattctc gacgtggtat catgatttat actcgtccat
3061 ctatgcagtt tgactataat aaagatggta tgtcagattt catgtgcacg aatacagtac
3121 aatacctgat tcgtgacaag aaaatcaatg cggttgttaa catgcgctca aatgacgtgg
3181 tcttcggatt ccgtaatgat tatgcatggc aaaaatacgt attagataaa ttagtatctg
3241 atttgaatgc aggtgattca actcgccagt ataaagcagg ttccattata tggaatgttg
3301 gaagcctaca tgtgtattct cgtcattttt atttggttga tcattggtgg aaaactggcg
3361 aaacacatat ttcgaaaaaa gattatgtag gaaaatacgc ttaatcgttt aacataaaag
3421 gaataatatg aaaattgcta taattaatat gggtaataat gttattaatt ttaaaactgt
3481 tccatcttct gaaactattt atctttttaa agttatttct gaaatgggtc ttaatgtcga
3541 c
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