GenBank-Updates@genbank.bio.net (09/12/90)
LOCUS ECACELS 1224 bp ds-DNA BCT 12-SEP-1990 DEFINITION E.carotovora beta(1,4)-glucan glucanohydrolase (celS) gene, complete cds. ACCESSION M32399 KEYWORDS beta(1,4)-glucan glucanohydrolase; endoglucanase. SOURCE E.carotovora carotovora (strain SCC3193) DNA, clone pHSK25. ORGANISM Erwinia carotovora Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively anaerobic rods; Enterobacteriaceae. REFERENCE 1 (bases 1 to 1224) AUTHORS Saarilahti,H.T., Henrissat,B. and Palva,E.T. TITLE CelS: A novel endoglucanase identified from Erwinia carotovora subsp. carotovora JOURNAL Gene 90, 9-14 (1990) STANDARD full staff_entry COMMENT Draft entry and computer-readable sequence [1] kindly submitted by H.T.Saarilahti, 02-MAR-1990. FEATURES from to/span description pept 208 1002 endoglucanase (celS) precursor sigp 208 303 endoglucanase signal peptide matp 304 999 endoglucanase binding 196 200 ribosome binding site BASE COUNT 353 a 278 c 253 g 340 t ORIGIN 1 taaaattaac accctgattt tatttcacat tattcatatt tattaagact ttaatcattt 61 ccccatgaat tggattattc cgacagagtg gaaataacat aaccagttaa actaaccaca 121 tctcagactt gatcatagtc acgtgataac gatgtgttaa ctttcagatt aaaacacctc 181 aagaatatat ttaaaaagag atttattatg cagactgtga atacccagcc acaccgtatt 241 ttccgcgtat tattgcccgc agtattttct tccttgctac tctcatcact aacagtgtct 301 gctgccagtt catcaaacga tgcggataaa ctctatttcg gtaataataa atattaccta 361 ttcaataacg tatggggaaa agatgaaata aaaggatggc agcaaacgat tttctataat 421 agcccaatta gcatggggtg gaactggcac tggccaagca gtacccacag tgttaaagcc 481 tatccttcac tggtgagcgg ttggcactgg acggcgggct atacggaaaa tagcggattg 541 ccgatacaat tatccagtaa taagagtatc accagtaacg ttacttattc tatcaaggct 601 acgggtactt acaacgccgc ttacgacatt tggttccaca ctaccgataa agccaactgg 661 gactcgtcgc ctaccgacga attgatgatt tggctaaatg ataccaatgc aggccctgcg 721 ggtgattata ttgaaaccgt tttcctcggc gacagcagtt ggaacgtatt caaaggctgg 781 atcaacgcgg ataatggtgg aggttggaat gtcttttcct ttgtccatac ttccggcacc 841 aacagtgcat cactcaatat tcgtcacttc accgactatt tggtgcagac aaagcaatgg 901 atgagcgatg aaaaatatat cagcagcgtt gagtttggca ccgagatctt tggcggcgat 961 gggcagatcg acatcaccga atggcgcgta gacgtaaaat agcgcgtctg gcgttgctat 1021 tcctccccgt tcagccgcaa taaacacgtt aacgtagagc tgaaacggat tggtctcatc 1081 gacgttctct tttcattttc agttcagaca gacgttgccc gttctttagg cgggttcgct 1141 tccccccaca acaaaattcg tgagctgtgg cacgtaaatt tcattagcgt tatgataatc 1201 ccctcggcta atcaggaatg tccc //