GenBank-Updates@genbank.bio.net (09/14/90)
LOCUS COBSPIKB 1299 bp ss-RNA VRL 14-SEP-1990
DEFINITION Bovine coronavirus 4.9, 4.8, 12.7 and 9.5 kD nonstructural,
and integral membrane protein, (5' end), and spike protein (3' end)
mRNAs.
ACCESSION M31054
KEYWORDS integral membrane protein; spike protein: nonstructural protein.
SOURCE Bovine coronavirus (strain Mebus), cDNA to viral RNA.
ORGANISM Bovine coronavirus
Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
Coronaviridae.
REFERENCE 1 (bases 1 to 1299)
AUTHORS Abraham,S., Kienzle,T.E., Lapps,W.E. and Brian,D.A.
TITLE Sequence and expression analysis of potential nonstructural
proteins of 4.9, 4.8, 12.7 and 9.5 kDa encoded between the spike
and membrane protein genes of the bovine coronavirus
JOURNAL Virology 177, 488-495 (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by D.A.Brian, 02-JAN-1990.
FEATURES from to/span description
pept < 1 334 spike protein
pept 324 455 nonstructural 4.9 kDa protein (put.)
pept 491 628 nonstructural 4.8 kDa protein (put.)
pept 712 1041 nonstructural 12.7 kDa protein (put.)
pept 1028 1282 nonstructural 9.7 kDa protein (put.)
pept 1297 > 1299 integral membrane protein
site 1 6 transcription start consensus 4.9 and 4.8 kD
proteins
site 631 636 transcription start consensus 12.7 kD protein
site 899 904 transcription start consensus 9.5 kD protein
BASE COUNT 371 a 202 c 257 g 469 t
ORIGIN
1 ccaaacatca gtggcaccag atttgtcact tgattatata aatgttacat tcttggacct
61 acaagatgaa atgaataggt tacaggaggc aataaaagtt ttaaatcaga gctacatcaa
121 tctcaaggac attggtacat atgagtatta tgtaaaatgg ccttggtatg tatggctttt
181 aattggcttt gctggtgtag ctatgcttgt tttactattc ttcatatgct gttgtacagg
241 atgtgggact agttgtttta agatatgtgg tggttgttgt gatgattata ctggacacca
301 ggagttagta attaaaacat cacatgacga ctaagttcgt ctttgattta ttggctcctg
361 acgatatatt acatcccttc aatcatgtga agctaattat aagacccatt gaggtcgagc
421 atattataat agctaccaca atgcctgctg tttagtgggt actgtgtctt atataactag
481 taaacctgta atgccaatgg ctacaaccat tgacggtaca gattatacta atattatgcc
541 tagtactgtt tctacaacag tttatttagg ctgttctata ggtattgaca ctagcaccac
601 tggttttacc tgtttttcac ggtactagtt ccaaaccata ttataattta ggtagacctt
661 ataactttaa gcattattaa ttgccaaagt ttctaaggtc acgccctagt aatggacatc
721 tggagacctg agattaaata tctccgttat actaacggtt ttaatgtctc agaattagaa
781 gatgcttgtt ttaaatttaa ctataaattt cctaaagtag gatattgtag agttcctagt
841 catgcttggt gccgtaatca aggtagcttt tgtgctacac tcactcttta tggcaaatcc
901 aaacattatg ataaatattt tggagtaata actggtttta cagcattcgc taatactgta
961 gaggaggctg ttaacaaact ggttttctta gctgttgact ttattacttg gcggagacag
1021 gagttaaatg tttatggctg atgcttattt tgcagacact gtgtggtatg tggggcaaat
1081 aatttttata gttgccattt gtttattggt tataatagtt gtagtggcat ttttggcaac
1141 ttttaaattg tgtattcaac tttgcggtat gtgtaatacc ttaggactgt ccccttctat
1201 ttatgtgttt aatagaggta ggcagtttta tgagttttac aacgatgtaa aaccaccagt
1261 tcttgatgtg gatgacgttt agttaatcca aacattatg
//